Abstract
Atypical kinase family, ABC1 (the activity of bc1 complex) genes play essential roles in plant growth, development and responses to be environmental stresses. Here, a novel gene, AtSIA1 (A. thaliana Salt-Induced ABC1 kinase 1) was cloned and characterized in Arabidopsis. Semi-quantitative RT-PCR analysis revealed that it constitutively expressed and mainly induced by salt treatment. AtSIA1-GFP fusion analysis showed that AtSIA1 is localized in chloroplasts. Transgenic plants with overexpression of AtSIA1 show higher tolerance to salt stress than that of the Col-0 and the knock-out mutant sia1 during seedling growth. Taken together, our results suggested that AtSIA1 may be involved in salt stress tolerance in Arabidopsis.
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Abbreviations
- ABC1 :
-
the activity of bc1 complex
- AtSIA1 :
-
A. thaliana Salt-Induced ABC1 kinase 1
- RT-PCR:
-
Reverse transcription polymerase chain reaction
- GFP:
-
Green fluorescent protein
- AtOSA1 :
-
A. thaliana oxidative stress related
- ABC1 :
-
like protein
- TaABC1 :
-
Triticum aestivum L. activity of bc1 complex
- 35S::AtSIA1 :
-
AtSIA1 overexpression transgenic plants under the control of the constitutively active cauliflower mosaic virus 35S promoter
- sia1 :
-
T-DNA insertion AtSIA1 mutant line
References
Bousquet I., Dujardin G. & Slonimski P. 1991. ABC1, a novel yeast nuclear gene, has a dual function in mitochondria: it suppresses a cytochrome b mRNA translation defect and is essential for the electron transfer in the bc 1 complex. EMBO J. 10: 2023–2031.
Brasseur G., Tron G., Dujardin G., Slonimski P.P. & Brivet-Chevillotte P. 1997. The nuclear ABC1 gene is essential for the correct conformation and functioning of the cytochrome bc1 complex and the neighbouring complexes II and IV in the mitochondrial respiratory chain. Eur. J. Biochem. 246: 103–111.
Cabrera R.I. 2003. Dermarcating salinity tolerance in greenhouse rose production. Acta Horticul. 609: 51–57.
Cardazzo B., Hamel P., Sakamoto W., Wintz H. & Dujardin G. 1998. Isolation of an Arabidopsis thaliana cDNA by complementation of a yeast ABC1 deletion mutant deficient in complex III respiratory activity. Gene 221: 117–125.
Clough S.J. & Bent A.F. 1998. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16: 735–743.
Hsieh E., Dinoso J. & Clarke C. 2004. AtRNATRP gene mediates the suppression of cbs2–223 previously attributed to ABC1/COQ8. Biochem. Bioph. Res. Com. 317: 648–653.
Jasinski M., Sudre D., Schansker G., Schellenberg M., Constant S., Martinoia E. & Bovet L. 2008. AtOSA1, a member of the ABC1-like family, as a new factor in cadmium and oxidative stress response. Plant Physiol. 147: 719–731.
Macinga D.R., Cook G.R., Poole R.K. & Rather ’P.N. 1998. Identification and characterization of aarF, a locus required for production of ubiquinone in Providencia stuartii and Escherichia coli and for expression of 29-N-acetyltransferase in P. stuartii. J. Bacteriol. 180: 128–135.
Nelson B.K., Cai X. & Nebenfuhr A. 2007. A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants. Plant J. 51: 1126–1136.
Ouyang S.Q., Liu Y.F., Liu P., Lei G., He S.J., Ma B., Zhang W.K., Zhang J.S. & Chen S.Y. 2010. Receptor-like kinase OsSIK1 improves drought and salt stress tolerance in rice (Oryza sativa) plants. Plant J. 62: 316–329.
Wang C.X., Jing R.L., Mao X.G., Chang X.P. & Li A. 2011. TaABC1, a member of the activity of bc1 complex protein kinase family from common wheat, confers enhanced tolerance to abiotic stresses in Arabidopsis. J. Exp. Bot. 62: 1299–1311.
Yang S.G., Zeng X.Q., Li T., Liu M., Zhang S.C., Gao S.J., Wang Y.Q., Peng C.L., Li L. & Yang C.W. 2012a. AtACDO1, an ABC1-like kinase gene, is involved in chlorophyll degradation and the response to photooxidative stress in Arabidopsis. J. Exp. Bot. Pubmed ID: 22447966.
Yang S.G., Li T., Liu M., Gao S.J., Yang S.Y., Li L. & Yang C.W. 2012b. Phylogenetic, structure and expression analysis of ABC1Ps gene family in rice. Biol. Plantarum. Doi: 10.1007/s 10535-012-0247-3.
Ytterberg A.J., Peltier J.B. & van Wijk K.J. 2006. Protein pro-filing of plastoglobules in chloroplasts and chromoplasts. A surprising site for differential accumulation of metabolic enzymes. Plant Physiol. 140: 984–997.
Zhu J.K. 2001. Plant salt tolerance. Trends Plant Sci. 6: 66–71.
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Yang, S., Zhang, Q., Li, T. et al. AtSIA1, an ABC1-like kinase, regulates salt response in Arabidopsis . Biologia 67, 1107–1111 (2012). https://doi.org/10.2478/s11756-012-0115-9
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DOI: https://doi.org/10.2478/s11756-012-0115-9