Abstract
The water-soluble sunscreen 2-phenylbenzimidazole-5-sulfonic acid (PBS) was quantified in a sun-care product and water samples by thin layer chromatography followed by densitometric scanning in fluorescence mode (cut-off filter 370 nm, wavelength of excitation — 300 nm). Normal phase TLC was performed on silica gel 60 as stationary phase. Mobile phase used was ethyl acetate-ethanol-water 70:35:30 (v/v/v). The limit of detection (LOD) was 0.0004 μg spot-1, and the limit of quantification (LOQ) was — 0.001 µg spot−1 without any sample pre-concentration.
References
A. Salvador, A. Chisvert, Anal. Chim. Acta 537, 1 (2005)
A. Chisvert, M.T. Vidal, A. Salvador, Anal. Chim. Acta 464, 295 (2002)
J.S. Azevedo, N.S. Viana Jr, C.D. Vianna Soares, Il Farmaco 54, 573 (1999)
P.G. Pietta, A. Bruno, P.L. Mauri, C. Gardana, R. Maffei-Facino, M. Carini, J. Pharm. Biomed. Anal. 13, 229 (1995)
C.W. Kampfl, T. Leitner, J. Sep. Sci. 26, 1259 (2003)
C.W. Klampf, T. Leitner, E.F. Hilder, Electrophoresis 23, 2424 (2002)
Z. Leon, A. Balaguer, A. Chisvert, A. Salvador, M. Herraez, O. Diez, Anal. Bioanal. Chem. 391, 859 (2008)
M.T. Vidal, A. Chisvert, A. Salvador, Talanta 59, 591 (2003)
G. Yener, J. Hadgraft, W.J. Pugh, Acta Pharm. Turcica 40, 27 (1998)
R. Rodil, J.B. Quintana, P. Lopez-Mahia, S. Muniategui-Lorenzo, D. Prada-Rodriguez, Anal. Chem. 80, 1307 (2008)
A. Nieto, F. Borrull, R.M. Marce, E. Pocurull, J. Chromatogr. A 1216, 5619 (2009)
A.W. Sobanska, J.Pyzowski, J. Planar Chromatogr. 25, 344 (2012)
J.J. Inbaraj, P. Bilski, C.F. Chignell, Photochem. Photobiol. 75, 107 (2002)
A.W. Sobanska, E. Brzezinska, Acta Pol. Pharm. 69, 791 (2012)
K. Ferenczi-Fodor, B. Renger, Z. Vegh, J. Planar Chromatogr. 23, 173 (2010)
ICH Harmonized Tripartite Guideline: Validation of Analytical Procedures: Text and Methodology, Q2 (R1) (Geneva, 2005) http://www.ich.org
P. Konieczko, J. Namiesnik (Eds.), Ocena i kontrola jakości wyników pomiarów analitycznych (Evaluation and quality control of analytical results) (Wydawnictwo Naukowo-Techniczne, Warszawa 2007) pp. 269–288 (In Polish)
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Sobanska, A.W., Derecka, K. & Pyzowski, J. Quantification of sunscreen 2-phenylbenzimidazole-5-sulfonic acid in cosmetic products and water samples by HPTLC/densitometry with fluorescent detection. cent.eur.j.chem. 11, 133–136 (2013). https://doi.org/10.2478/s11532-012-0151-x
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DOI: https://doi.org/10.2478/s11532-012-0151-x