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Immobilization of lactate dehydrogenase on tetraethylorthosilicate-derived sol-gel films for application to lactate biosensor

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Abstract

Tetraethylorthosilicate (TEOS)-derived sol-gel films were utilized for the immobilization of lactate dehydrogenase (LDH) by physical adsorption and sol-gel/LDH/sol-gel sandwich configuration. An attempt was made to ascertain the optimum pH and temperature for the immobilized LDH. It was shown that TEOS-derived sol-gel films containing physically adsorbed LDH exhibited linearity from 0.5 to 4 mM, whereas those containing LDH in sandwich configuration showed linearity from 0.5 to 3 mM l-lactate. These sol-gel films, immobilized with LDH, were found to be stable for about 4 weeks at 4–10°C.

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Authors and Affiliations

  1. Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K. S. Krishnan Marg, 110012, New Delhi, India

    Asha Chaubey, Manju Gerard & B. D. Malhotra

  2. Department of Biochemistry, L.L.R.M. Medical College, 250004, Meerut, India

    Asha Chaubey & V. S. Singh

Authors
  1. Asha Chaubey
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  2. Manju Gerard
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  3. V. S. Singh
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  4. B. D. Malhotra
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Correspondence to Asha Chaubey.

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Chaubey, A., Gerard, M., Singh, V.S. et al. Immobilization of lactate dehydrogenase on tetraethylorthosilicate-derived sol-gel films for application to lactate biosensor. Appl Biochem Biotechnol 96, 303–311 (2001). https://doi.org/10.1385/ABAB:96:1-3:303

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  • DOI: https://doi.org/10.1385/ABAB:96:1-3:303

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