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Monitoring of Paclitaxel, Taxine B and 10-Deacethylbaccatin III in Taxus baccata L. by Nano LC–FTMS and NMR Spectroscopy

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Abstract

In this work, an aqueous extraction method has been combined with a preparative LC for isolation of 10-deacetylbaccatin III (10-DAB) from leaves of Taxus baccata L. The extracted aqueous solution showed two major peaks in LC. Semi-preparative-LC was used for isolation of these peaks. Nano liquid chromatography-Fourier transform mass spectrometry and nuclear magnetic resonance showed that these peaks belong to taxine B and 10-DAB in the extracted aqueous solution. The residual sample of aqueous extraction solution was extracted again by MAE. The amount of paclitaxel in Taxus baccata L. was compared with and without aqueous extraction and results showed that paclitaxel was stable and had no significant difference in concentrations during this aqueous extraction process.

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Acknowledgments

This work has been supported by a grant from the Shahid Beheshti University Research Council.

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Authors and Affiliations

  1. Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C, Evin, Tehran, Iran

    Alireza Ghassempour, Hassan Rezadoost & Alireza Mashouf

  2. Pharmaceutical and Medicinal Chemistry Department, National Research Centre, Dokki, Cairo, 12311, Egypt

    Hassan Y. Aboul-Enein

  3. Institute for Inorganic and Analytical Chemistry, Justus Liebig University, Giessen, Germany

    Bernhard Spengler & Andreas Römpp

Authors
  1. Alireza Ghassempour
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  2. Hassan Rezadoost
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  3. Alireza Mashouf
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  4. Hassan Y. Aboul-Enein
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  5. Bernhard Spengler
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  6. Andreas Römpp
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Corresponding authors

Correspondence to Alireza Ghassempour or Hassan Y. Aboul-Enein.

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Ghassempour, A., Rezadoost, H., Mashouf, A. et al. Monitoring of Paclitaxel, Taxine B and 10-Deacethylbaccatin III in Taxus baccata L. by Nano LC–FTMS and NMR Spectroscopy. Chroma 72, 833–839 (2010). https://doi.org/10.1365/s10337-010-1743-2

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  • DOI: https://doi.org/10.1365/s10337-010-1743-2

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