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LC-MS-MS Method for the Determination of Protoveratrine A in Rat Plasma

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Abstract

The present experiments were designed for the development of a liquid chromatographic coupled with tandem mass spectrometric method for the quantitation of trace amount of protoveratrine A in rat plasma. A liquid-liquid extraction protocol was involved in sample preparation. Quantitation was achieved using multiple-reaction-monitoring (MRM) mode. The method was validated with the concentration range 0.02–40 ngmL−1 in plasma. Intra-/inter-day accuracy and precision as well as the LOD and the lower limit of quantitation were acceptable. The method was further applied to analyze protoveratrine A in rat plasma samples.

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Acknowledgements

The work was supported by the program for Changjiang Scholars and Innovative Research Team in University (PCSIRT), “973” program of China (2007CB507400), National 863 Program (2006AA02Z338) and in part by the Scientific Foundation of Shanghai China (05DZ19733, 06DZ19717, 06DZ19005).

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Authors and Affiliations

  1. Department of Natural Medicinal Chemistry, School of Pharmacy, Second Military Medical University, Shanghai, 200433, People’s Republic of China

    Hai-Yun Li, Wen Xu, Hui-Liang Li, Wei-Dong Zhang & Li-Wei Hu

  2. School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200030, People’s Republic of China

    Wei-Dong Zhang

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  1. Hai-Yun Li
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  2. Wen Xu
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  3. Hui-Liang Li
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  4. Wei-Dong Zhang
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Correspondence to Wei-Dong Zhang.

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Li, HY., Xu, W., Li, HL. et al. LC-MS-MS Method for the Determination of Protoveratrine A in Rat Plasma. Chroma 69, 523–529 (2009). https://doi.org/10.1365/s10337-008-0936-4

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  • DOI: https://doi.org/10.1365/s10337-008-0936-4

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