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Improving Quantitative Measurements for the Evaporative Light Scattering Detector

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Abstract

A study was undertaken to evaluate a generic calibration routine for the evaporative light scattering detector (ELSD). The calibration routine was designed for use with gradient HPLC-ELSD in order to adjust for the effects of the non-linear response curve and the variation in response with mobile phase composition. A single calibrant was employed with an automated injection protocol to enable rapid capture of the calibration information. The study assessed the validity of the calibration method and the suitability of the calibrant for the quantitation and purity estimation of a series of structurally unrelated compounds. The calibration routine was demonstrated to improve the quality of quantitative estimates and purity estimates given by the gradient HPLC-ELSD. The average error in quantitation for the series of materials was 8.3%.

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Acknowledgments.

The authors would like to thank George Perkins and Frank Pullen (Pfizer Global R & D) without whose help and support this project could not have been completed. Thanks are extended to Polymerlabs Ltd (Church Stretton, UK) and Anachem (Luton, UK) for loan of ELS detectors. Special thanks to Dr Donald Daley (Argenta) for convincing us that our initial ELSD performance observations were correct and that processes to correct them were viable.

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Authors and Affiliations

  1. Medway Sciences, Natural Resources Institute, Medway University Campus, Central Avenue, Chatham Maritime, Kent, ME4 4TB, UK

    B. T. Mathews, J. C. Mitchell & M. J. Snowden

  2. Pfizer Global Research and Development, Ramsgate Road, Sandwich, Kent, CT13 9NJ, UK

    B. T. Mathews, P. D. Higginson, R. Lyons, N. W. Sach, M. R. Taylor & A. G. Wright

Authors
  1. B. T. Mathews
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  2. P. D. Higginson
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  3. R. Lyons
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  4. J. C. Mitchell
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  5. N. W. Sach
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  6. M. J. Snowden
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  7. M. R. Taylor
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  8. A. G. Wright
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Correspondence to B. T. Mathews.

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Mathews, B., Higginson, P., Lyons, R. et al. Improving Quantitative Measurements for the Evaporative Light Scattering Detector. Chromatographia 60, 625–633 (2004). https://doi.org/10.1365/s10337-004-0441-3

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  • DOI: https://doi.org/10.1365/s10337-004-0441-3

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