Abstract
LC–DAD coupled with electrospray tandem mass spectrometry (LC–ESI–MS–MS) has been used to evaluate the quality of the traditional Chinese medicine Huang-Lian-Jie-Du-Tang (HLJDT). Twenty-five chromatographic peaks were obtained from a C18 analytical column by gradient elution with acetonitrile and formate buffer (containing 0.5% formic acid) at a flow rate of 1.0 mL min−1. The linearity, precision, and accuracy of the data obtained were acceptable. Thirteen components were identified by ESI–MS, and seven of these were quantitatively analyzed by LC–DAD. The method was used to analyze ten batches of HLJDT, and both chromatographic fingerprints and quantitative data were used to evaluate the quality of the HLJDT. It was concluded that this LC–DAD–ESI–MS method enables more fully validated and complete evaluation and monitoring of the quality of HLJDT.
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Acknowledgment
The work was supported by the program for Changjiang Scholars and Innovative Team in University (PCSIRT), the NCET Foundation, NSFC (30725045), the National 863 Program (2006AA02Z338), the China Postdoctoral Science Foundation (20070410711), the “973” program of China (2007CB507400), the Shanghai Leading Academic Discipline Project (B906), and, in part, by the Scientific Foundation of Shanghai China (07DZ19728, 06DZ19717, 06DZ19005).
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Dou, S., Liu, L., Jiang, P. et al. LC–DAD and LC–ESI–MS Chromatographic Fingerprinting and Quantitative Analysis for Evaluation of the Quality of Huang-Lian-Jie-Du-Tang. Chroma 69, 659–664 (2009). https://doi.org/10.1365/s10337-008-0945-3
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DOI: https://doi.org/10.1365/s10337-008-0945-3