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Bioactive/Natural Polymeric Scaffolds Loaded with Ciprofloxacin for Treatment of Osteomyelitis

  • Research Article
  • Theme: Recent Trends in the Development of Chitosan-Based Drug Delivery Systems
  • Published:
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ABSTRACT

Local delivery of antibiotic into injured bone is a demand. In this work, different scaffolds of chitosan (C) with or without bioactive glass (G) were prepared using the freeze-drying technique in 2:1, 1:1, and 1:2 weight ratios. Chitosan scaffolds and selected formulas of chitosan to bioglass were loaded with ciprofloxacin in 5%, 10%, and 20% w/w. Scaffold morphology showed an interconnected porous structure, where the glass particles were homogeneously dispersed in the chitosan matrix. The kinetic study confirmed that the scaffold containing 1:2 weight ratio of chitosan to glass (CG12) showed optimal bioactivity with good compromise between Ca and P uptake capacities and Si release rate. Chitosan/bioactive glass scaffolds showed larger t 50 values indicating less burst drug release followed by a sustained drug release profile compared to that of chitosan scaffolds. The cell growth, migration, adhesion, and invasion were enhanced onto CG12 scaffold surfaces. Samples of CG12 scaffolds with or without 5% drug induced vascular endothelial growth factor (VEGF), while those containing 10% drug diminished VEGF level. Only CG12 induced the cell differentiation (alkaline phosphatase activity). In conclusion, CG12 containing 5% drug can be considered a biocompatible carrier which would help in the localized osteomyelitis treatment.

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ACKNOWLEDGMENTS

This work is financially supported by Science and Technology Development Fund (STDF) Egypt, project number 5024.

Author information

Authors and Affiliations

  1. Ceramics Department, National Research Centre (NRC), El Bohouth St. 33, 12622 Dokki, Cairo, Egypt

    Amany A. Mostafa

  2. Nanomedicine and Tissue Engineering Laboratory, Medical Research Centre of Excellence, NRC, El Bohouth St., 12622 Dokki, Cairo, Egypt

    Amany A. Mostafa

  3. Chemical Engineering Department, National Research Centre (NRC), El Bohouth St., Dokki, 12622, Cairo, Egypt

    Mayyada M. H. El-Sayed

  4. Chemistry Department, American University in Cairo, New Cairo, 11835, Egypt

    Mayyada M. H. El-Sayed

  5. Pharmaceutical Technology Department, National Research Centre, Dokki, Cairo, Egypt

    Azza A. Mahmoud

  6. Pharmaceutics and Pharmaceutical Technology Department, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt, Cairo, Egypt

    Azza A. Mahmoud

  7. Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, NRC, El Bohouth St. 33, Dokki, 12622, Cairo, Egypt

    Amira M. Gamal-Eldeen

  8. Biochemistry Department, NRC, Dokki, Cairo, Egypt

    Amira M. Gamal-Eldeen

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  1. Amany A. Mostafa
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  2. Mayyada M. H. El-Sayed
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  3. Azza A. Mahmoud
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  4. Amira M. Gamal-Eldeen
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Correspondence to Amany A. Mostafa or Amira M. Gamal-Eldeen.

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Guest Editors: Claudio Salomon, Francisco Goycoolea, and Bruno Moerschbacher

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Mostafa, A.A., El-Sayed, M.M.H., Mahmoud, A.A. et al. Bioactive/Natural Polymeric Scaffolds Loaded with Ciprofloxacin for Treatment of Osteomyelitis. AAPS PharmSciTech 18, 1056–1069 (2017). https://doi.org/10.1208/s12249-016-0605-0

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  • DOI: https://doi.org/10.1208/s12249-016-0605-0

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