A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578

Genetically encoded calcium ion (Ca²⁺) indicators (GECIs) are indispensable tools for measuring Ca²⁺ dynamics and neuronal activities in vitro and in vivo. Red fluorescent protein (RFP)-based GECIs have inherent advantages relative to green fluorescent protein-based GECIs due to the longer wavelength light used for excitation. Longer wavelength light is associated with decreased phototoxicity and deeper penetration through tissue. Red GECI can also enable multicolor visualization with blue- or cyan-excitable fluorophores.

Results

Here we report the development, structure, and validation of a new RFP-based GECI, K-GECO1, based on a circularly permutated RFP derived from the sea anemone Entacmaea quadricolor. We have characterized the performance of K-GECO1 in cultured HeLa cells, dissociated neurons, stem-cell-derived cardiomyocytes, organotypic brain slices, zebrafish spinal cord in vivo, and mouse brain in vivo.

Conclusion

K-GECO1 is the archetype of a new lineage of GECIs based on the RFP eqFP578 scaffold. It offers high sensitivity and fast kinetics, similar or better than those of current state-of-the-art indicators, with diminished lysosomal accumulation and minimal blue-light photoactivation. Further refinements of the K-GECO1 lineage could lead to further improved variants with overall performance that exceeds that of the most highly optimized red GECIs.

Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting

Article Open access 16 February 2016

Genetically encoded fluorescent indicators for imaging intracellular potassium ion concentration

Article Open access 14 January 2019

NTnC-like genetically encoded calcium indicator with a positive and enhanced response and fast kinetics

Article Open access 15 October 2018

Background

Protein engineering efforts have yielded three major lineages of monomeric red fluorescent proteins (RFPs) derived from their naturally oligomeric precursors (Fig. 1a). One lineage comes from the Discosoma sp. mushroom coral RFP, DsRed, and includes the first monomeric RFP, mRFP1 [1], and the mRFP1-derived mFruit variants such as mCherry, mCherry2, mOrange, and mApple [2,3,4]. The second and third lineages stem from the sea anemone Entacmaea quadricolor RFPs eqFP578 [5] and eqFP611 [6], respectively. EqFP578 is the progenitor of the bright monomeric proteins TagRFP, TagRFP-T, mKate, mKate2, and the low-cytotoxicity variant FusionRed [5, 7,8,9]. Engineering of eqFP611 produced mRuby, mRuby2, and mRuby3, a line of RFPs with relatively large Stokes shift and bright red fluorescence [10,11,12]. Together, these three lineages of monomeric RFPs are commonly used in a variety of fluorescence imaging applications and have served as templates for developing red fluorescent indicators of various biochemical activities [13].

Among the many fluorescent-protein-based indicators of biochemical activity, genetically encoded calcium ion (Ca²⁺) indicators (GECIs) are particularly versatile tools. Most notably, they enable imaging of neuronal activity in contexts ranging from dissociated neurons in vitro to brain activity in behaving animals [14]. Green fluorescent GCaMPs, in particular, have proven extremely useful for imaging Ca²⁺ activities in various neural systems [15,16,17]. The development of the first single RFP-based Ca²⁺ indicators, the DsRed-derived R-GECO1 [18] and eqFP611-derived RCaMP1h [19], unlocked new opportunities for simultaneous multicolor optical imaging. Further engineering of R-GECO1 produced a number of improved and altered variants, including R-CaMP1.07, R-GECO1.2, CAR-GECO1, O-GECO1, R-CaMP2, and REX-GECO1 [20,21,22,23]. Optimization of R-GECO1 and RCaMP1h for detection of neuronal action potentials produced jRGECO1a, jRCaMP1a, and jRCaMP1b [24]. One limitation of the R-GECO series of GECIs is that they inherited undesirable blue-light-activated photoswitching behavior that was also present in the DsRed-derived template (mApple) from which they were engineered [3, 19, 25, 26]. Accordingly, when combining the R-GECO series of Ca²⁺ indicators with optogenetic actuators, extra care must be taken to differentiate true responses from artifacts caused by photoactivation [19, 21]. RCaMP variants do not show photoswitching under blue illumination but they are less responsive than R-GECO variants in terms of fluorescence change upon Ca²⁺ binding [19, 24]. Like many DsRed-derived RFPs, R-GECO variants have a propensity to accumulate in lysosomes and form brightly fluorescent (but non-functional) puncta during long-term neuronal expression [27,28,29]. These puncta can complicate image analysis and may compromise long-term cell viability. Notably, transgenic mice expressing RCaMP1.07 (equivalent to R-GECO1 K47V, T49V with a C-terminal peptide extension) exhibit stable and widespread neuronal expression, despite the formation of numerous puncta [30].

The drawbacks associated with the DsRed- and eqFP611-derived GECIs motivated us to explore a new RFP template for development of red GECIs. As mentioned above, some DsRed-derived RFPs, such as mOrange and mCherry, have been reported to exhibit relatively dim fluorescence and/or puncta formation, when transgenically expressed in mice brains [31]. In contrast, eqFP578-derived RFPs TagRFP-T and mKate2 have been reported to exhibit bright fluorescence without puncta formation in vivo [31]. The eqFP611-derived mRuby has been reported to have the highest cytotoxicity among various RFPs [9]. Based on these literature reports, and reinforced by observations in our own lab, we reasoned that using an eqFP578-derived RFP as a template for the development of a new red GECI could potentially address the limitations of R-GECO, and possibly offer better performance in vivo. Here we report our efforts to design, engineer, characterize, and validate a new red GECI, K-GECO1, based on the eqFP578 variant FusionRed [9].

Results

Design and engineering of K-GECO1

We initially selected two eqFP578-derived RFPs, mKate2 [8] and its low-cytotoxicity variant FusionRed [9], as templates to construct a red Ca²⁺ indicator. Both mKate2 and FusionRed scaffolds were circularly permutated (cp) at residue Ser143 (numbering according to mKate crystal structure [32], PDB: 3BXB), which is the same permutation site used in GCaMPs and R-GECOs [18, 33]. Both cpRFPs were genetically inserted between N-terminal chicken myosin light-chain kinase peptide RS20 and C-terminal calmodulin (CaM) from R-GECO1. The resulting indicator prototype based on the cpmKate2 scaffold was not fluorescent, in accordance with a previous study of mKate circular permutation [34], and therefore, no further optimization was pursued. In contrast, the cpFusionRed-based design (designated K-GECO0.1) (Fig. 1b), was dimly fluorescent when expressed in Escherichia coli colonies for 48 h at room temperature. The extracted protein showed a 20% fluorescence emission intensity increase upon addition of Ca²⁺. To improve the function of this prototype indicator further, we first performed random mutagenesis of the peptide linker between the RS20 peptide and cpFusionRed (linker1), which is Pro30-Val31-Val32 as in R-GECO1 (numbered as in Additional file 1: Figure S1). Screening of this targeted mutagenesis library led to identification of the Pro30Lys-Val31Tyr-Val32Asn variant with visible red fluorescence in E. coli after overnight incubation. This variant, termed K-GECO0.2, exhibited a twofold fluorescence emission intensity increase upon Ca²⁺ binding. K-GECO0.2 was subjected to further directed protein evolution for brightness and to increase the Ca²⁺-induced fluorescence intensity change. In each round of directed evolution, error-prone polymerase chain reaction (EP-PCR) was used to create a variant library. After visual inspection of the plated library, the brightest fluorescent colonies were picked, cultured, and the protein purified and tested for its Ca²⁺ response. The pool of variants with the largest Ca²⁺-dependent fluorescence changes served as templates for the next round of evolution. After three rounds, an improved variant K-GECO0.5 was produced. Initial characterization of K-GECO0.5 indicated a relatively low Ca²⁺ affinity with a K_d close to 1 μM. To overcome this limitation, we engineered K-GECO0.6 using an approach similar to the one used by Inoue et al. to develop R-CaMP2 [23]. Following the strategy of Inoue et al., we incorporated the rat CaM-dependent kinase kinase peptide (ckkap) in place of RS20, and introduced GCaMP6 mutations Asn342Ser, Asp343Tyr, Thr344Arg, and Ser346Thr into the CaM domain [23]. An additional three rounds of directed evolution led to K-GECO0.9. In the final step of engineering, we performed saturation mutagenesis of the linker between cpFusionRed and CaM (linker2). Screening of the library identified a variant with linker2 changed from Thr265-Arg266 into Ser265-Asn266. This final variant was designated as K-GECO1 (Fig. 1b).

In vitro characterization of K-GECO1

The excitation and emission maxima of K-GECO1 are 568 and 594 nm, respectively, in the Ca²⁺-unbound state. In the Ca²⁺-bound state, these two maxima are slightly blue-shifted to 565 and 590 nm (Fig. 2a, Additional file 2: Table S1). K-GECO1 exhibits a 12-fold fluorescent intensity increase upon Ca²⁺ binding, with the extinction coefficient increasing from 19,000 to 61,000 M^-1cm^-1 and the quantum yield from 0.12 to 0.45 (Additional file 2: Table S1). The fluorescence spectra characteristics and Ca²⁺-induced fluorescence change of K-GECO1 are generally very similar to those of R-GECO1 (Additional file 2: Table S1). However, K-GECO1 is about twofold brighter than R-GECO1 under one-photon excitation. Ca²⁺ titration of purified K-GECO1 reveals that the protein has an apparent K_d of 165 nM with a Hill coefficient of 1.12 (Fig. 1b, Additional file 2: Table S1), similar to R-CaMP2 and other ckkap-based GECIs [23, 35].

K-GECO1 displayed moderate photoactivation when illuminated with either a 405-nm or 488-nm laser, in both the Ca²⁺-free and Ca²⁺-bound states. For Ca²⁺-bound K-GECO1, illuminating with 405-nm (1.76 W/cm²) or 488-nm (6.13 W/cm²) laser light for 1 s resulted in a ~20% increase in fluorescence as detected using 561-nm illumination. For Ca²⁺-free K-GECO1, 1 s of 405-nm (1.76 W/cm²) or 488-nm (6.13 W/cm²) laser light also resulted in a ~20% increase in fluorescence (Additional file 3: Figure S2a). Consistent with previous reports [19, 21], we observed a more pronounced photoactivation with R-GECO1, but not RCaMP1h, under similar illumination conditions (Additional file 3: Figure S2b–d).

K-GECO1 shows a strong two-photon excitation peak at approximately 1100 nm (Fig. 2c) in the Ca²⁺-bound state. A ~25-fold maximal increase of fluorescence signal, using two-photon excitation in the excitation region from 1050 to 1150 nm, occurs upon binding Ca²⁺ (Fig. 2c). The peak two-photon molecular brightness of K-GECO1 was compared with R-GECO1, using mCherry as a standard with 1060-nm excitation. The peak two-photon molecular brightness, defined as the maximum detected fluorescence count rate per emitting molecule [36], was obtained from the average fluorescence count rate and the average number of emitting molecules in the beam as determined by fluorescence correlation spectroscopy. Using this approach, K-GECO1 was found to be approximately 1.5-fold brighter than mCherry and over twofold brighter than R-GECO1 (Fig. 2d), which is consistent with the comparison of one-photon brightness for the Ca²⁺-bound state (Additional file 2: Table S1).

Crystal structure of K-GECO1

To gain insight into the molecular mechanism of K-GECO1 Ca²⁺ sensitivity and to assist future protein engineering efforts, we determined the X-ray crystal structure of K-GECO1 in the Ca²⁺-bound form. The structure was determined to 2.36-Å resolution by molecular replacement (Fig. 2e, Additional file 4: Table S2). The crystal structure reveals the distinctive features of the ckkap/CaM complex in K-GECO1 (and presumably in other ckkap-based GECIs) relative to other RS20/CaM-based GECIs, including R-GECO1 (Fig. 2f), RCaMP (Fig. 2g), and GCaMP6 (Fig. 2h). The major difference is that the binding orientation of the ckkap peptide to the CaM domain is opposite to that of RS20 to CaM [37, 38]. Another difference is that the RS20 peptide consists entirely of an α-helix in the CaM-binding region, whereas the CaM-binding region of ckkap consists of both an α-helical segment as well as a hairpin-like loop structure at its C-terminus [35].

Examination of the molecular interactions between the protein and the chromophore at the circular permutation site provides insights into the mechanism of Ca²⁺-dependent fluorescence modulation. The side chain of Asn32 of linker1 is in direct hydrogen bonding with the phenolate oxygen of the chromophore (Fig. 2i), and is positioned similarly to Ser143 of FusionRed, which engages in a similar interaction with the chromophore [9]. We reason that Asn32 plays a critical role in communicating the Ca²⁺-dependent conformational change in the ckkap/CaM domain to the chromophore in the cpRFP domain. Lys79 of R-GECO1 (Fig. 2j), Thr243 of RCaMP1h (Fig. 2k), and Arg376 of GCaMP6 (Fig. 2l) are likely to have similar roles in their respective mechanisms of fluorescence modulation. Saturation mutagenesis of Asn32 of K-GECO1 resulted in a library of variants that all had dimmer fluorescence and/or a smaller Ca²⁺-induced fluorescence intensity fold change. These results indicate that Asn is the optimal residue in this position.

Performance of K-GECO1 in cultured cells

To demonstrate the utility of K-GECO1 in imaging Ca²⁺ dynamics, we expressed it in cultured human cells, dissociated rat neurons, organotypic rat brain slices, zebrafish sensory neurons, and mouse primary visual cortex. We first recorded the response of K-GECO1 to changes in the cytoplasmic Ca²⁺ concentration in HeLa cells using established protocols (Fig. 3a) [39]. HeLa cells expressing K-GECO1 had maximum fluorescence intensity changes of 5.2 ± 1.1-fold (n = 44) on treatment with histamine, which is similar to the 4.9 ± 1.9-fold (n = 22) response previously reported for R-GECO1 expressing HeLa cells [18].

Next, we tested K-GECO1 in dissociated rat hippocampal neurons. The relatively low Ca²⁺ K_d of 165 nM for K-GECO1 is comparable to that of current best green GECI, GCaMP6s [17], which has been highly optimized for detection of neuronal Ca²⁺ transients. Cultured dissociated neurons expressing K-GECO1 had fluorescence distributed throughout the cytosol and nucleus, and exhibited close to twofold maximum increases for spontaneous Ca²⁺ changes (Fig. 3b). We did not observe intracellular fluorescent punctate structures, as have been observed for R-GECO1 and its variants [22, 27], in the cell bodies of dissociated neurons expressing K-GECO1 (Additional file 5: Figure S3a,b). We also did not observe noticeable photoactivation of K-GECO1 in neurons when illuminated with 0.5 W/cm² of 405-nm laser light. Under the same illumination conditions, R-GECO1 exhibited substantial photoactivation (Additional file 5: Figure S3c,d). The absence of photoactivation for K-GECO1 under these conditions might be due to the relative low laser intensity (0.5 W/cm²) compared with the intensity (1.76 W/cm²) used for in vitro characterization.

To compare the performance of K-GECO1 with other red GECIs in dissociated neurons, we performed an automated imaging assay with field stimulation as previously described [17, 24]. For a single action potential, K-GECO1 exhibited a similar response to jRGECO1a (Fig. 3c) and GCaMP6s [17], two of the most sensitive indicators currently available. The peak ΔF/F₀ amplitude of K-GECO1 with three or more action potentials was smaller than that of jRGECO1a, yet better than other red GECIs (Fig. 3d,e). In terms of the signal-to-noise ratio, K-GECO1 had similar performance to jRGECO1a, but less than that of jRCaMPa/b (Fig. 3f). K-GECO1 exhibits fast kinetics, with a half decay time that is faster than jRGECO1a and jRCaMP1a/b (Fig. 3g), and a half rise time that is similar to jRGECO1a but faster than jRCaMP1a/b (Fig. 3h).

As our in vitro characterization indicated that K-GECO1 has less blue-light photoactivation than R-GECO1, we tested its performance in human induced pluripotent stem cell–derived cardiomyocytes (iPSC-CMs) in combination with channel rhodopsin-2 (ChR2). As expected, transfected iPSC-CMs expressing K-GECO1 exhibited spontaneous Ca²⁺ oscillations (Fig. 4a). To compare photoactivation of K-GECO1 and R-GECO1 in iPSC-CMs, we illuminated transfected cells (GECI only, no ChR2) with 0.19 W/cm² of 470-nm LED light (Fig. 4b,c). Under these conditions, R-GECO1 exhibited a substantial photoactivation effect with a transient 200% increase in red fluorescence. Under the same illumination conditions, K-GECO1 had a negligible change in red fluorescence. When we co-transfected iPSC-CMs with both K-GECO1 and ChR2, blue-light stimulation reliably induced Ca²⁺ transients (Fig. 4d), demonstrating that the combination of K-GECO1 and ChR2 is feasible for all-optical excitation and imaging of iPSC-CMs.

Performance of K-GECO1 in organotypic brain slices

We further tested the performance of K-GECO1 by expressing it in organotypic slices of the newborn rat ventromedial nucleus (VMN) of the hypothalamus. Expression of K-GECO1 enabled visualization of both neuronal cell bodies and processes (Fig. 5a). We investigated the performance of K-GECO1 under pharmacological stimulation by adenosine triphosphate (ATP) (100 μM), which activates suramin-sensitive ATP receptors and induces an influx of extracellular Ca²⁺, thus increasing the cytosolic Ca²⁺ concentration [40]. Upon treatment with ATP, neurons expressing K-GECO1 underwent a mean increase in fluorescence intensity of 3.26 + 0.18-fold (n = 21) (Fig. 5b).

To compare the performance of K-GECO1 with the small molecule-based green cytosolic Ca²⁺ indicator, Fluo-4 AM, we loaded the dye into VMN neurons that were expressing K-GECO1 (Fig. 5c). When treated with ATP, these neurons (n = 3) exhibited a 3.01 + 0.86-fold increase in K-GECO1 fluorescence, but only a 0.70 + 0.12-fold increase in Fluo-4 fluorescence (Fig. 5d). In non-transfected cells stained with Fluo-4 AM, we did not observe any crosstalk from Fluo-4 AM into the red channel. Overall, K-GECO1 unravels robust responses to cytosolic Ca²⁺ concentration changes in neurons in organotypic brain slices.

In vivo Ca²⁺ imaging with K-GECO1

To test K-GECO1 in zebrafish spinal cord sensory neurons in vivo, we transiently expressed K-GECO1 in Rohon–Beard (RB) cells. Zebrafish RB cells have previously been used for in vivo GECI imaging and shown to fire a single spike in response to each electrical pulse to the skin [41]. Electrical stimulations were applied to trigger Ca²⁺ transients at 3 days post fertilization. Two-photon imaging with excitation at 1140 nm (Fig. 6a) revealed that K-GECO1 filled both the cytoplasm and nucleus in vivo in zebrafish RB neurons (Fig. 6b). Cytoplasmic K-GECO1 exhibited a ~40% fluorescence intensity increase to Ca²⁺ transients triggered by a single pulse stimulus (Fig. 6c). When the RB neurons were stimulated with 5 to 20 repetitive stimuli, 50–100% increases in K-GECO1 fluorescence were observed (Fig. 6d). As expected, the fluorescence response in the nucleus was diminished with respect to the response in the cytosol, and exhibited a slower recovery to baseline (Fig. 6c,d). Compared to the optimized red fluorescent indicator jRGECO1a, K-GECO1 showed decreased sensitivity in zebrafish in terms of stimulus-evoked fluorescence change (Fig. 6e,f), whereas the half decay time was comparable (Fig. 6g, h). Consistent with the results from dissociated neurons, an even distribution of the K-GECO1 red fluorescence in RB cells was observed in zebrafish neurons in vivo (Additional file 6: Figure S4a,b), while jRGECO1 exhibited fluorescence accumulations (Additional file 6: Figure S4c).

To evaluate K-GECO1 in the mouse primary visual cortex (V1) in vivo, V1 neurons were infected with adeno-associated virus (AAV) expressing nuclear export signal (NES) tagged K-GECO1 under the human synapsin-1 promoter (AAV-SYN1-NES-K-GECO1). The majority of V1 neurons can be driven to fire action potentials in response to drifting gratings. Eight-direction moving grating visual stimuli were presented to the contralateral eye (Fig. 7a). K-GECO1 expressing L2/3 neurons exhibited cytoplasmic red fluorescence (Fig. 7b), and two-photon imaging revealed visual-stimulus-evoked fluorescence transients in subsets of neurons (Fig. 7c). We compared the performance of K-GECO1 with other red GECIs using previously established metrics [17, 24]. The fraction of neurons detected as responsive in the visual cortex is higher for K-GECO1 than RCaMP1h, but lower than R-GECO1 and other optimized red indicators (Fig. 7d). The mean ΔF/F₀ at the preferred visual stimulus is reflective of indicator sensitivity. By this metric, K-GECO1 has sensitivity that is comparable to those of R-GECO1 and jRCaMP1a, but less than jRGECO1a (Fig. 7e). Lysosomal accumulation was previously observed in mouse V1 neurons labeled with jRGECO1a, but not in the ones with jRCaMP1a/b [24]. Fixed brain tissue sections, prepared as previously reported for jRGECO1a and jRCaMP1a/b [24], revealed no signs of lysosomal accumulation in K-GECO1-expressing V1 neurons (Additional file 7: Figure S5a). As with both jRGECO1a and jRCaMP1a/b, in vivo functional imaging of K-GECO1 did exhibit fluorescent clump-like structures (Additional file 7: Figure S5b), yet these structures were not observed in fixed sections of the same tissue. We are currently unable to explain this discrepancy. Overall, the results demonstrate that K-GECO1 can be used to report physiological Ca²⁺ changes in neurons in vivo with performance that matches or surpasses that of other first-generation red fluorescent Ca²⁺ indicators.

Discussion

Although green fluorescent GECIs are currently the most highly effective tools for in vivo visualization of neuronal signaling, we anticipate that they will one day be made redundant by red fluorescent GECIs due to the inherent advantages associated with longer wavelength fluorescence. The transmittance of tissue increases as the wavelength increases, so red fluorescent GECIs will enable imaging of neuronal activity deeper into brain tissue than is possible with green fluorescent GECIs, assuming all other properties are equivalent [24, 30]. In addition, red fluorescent GECIs enable multiparameter imaging in conjunction with green fluorescent indicators, and facilitate simultaneous imaging and optical activation when used in conjunction with blue-light activatable optogenetic actuators such as ChR2 [42]. However, as widely recognized [13, 19, 22, 24], red GECIs currently suffer from a number of limitations compared to the most highly optimized green GECIs (i.e., GCaMP6) [17]. These limitations include decreased sensitivity for RCaMP variants and complicated photophysics and lysosomal accumulation for R-GECO variants. As both green and red GECIs have analogous designs and contain identical Ca²⁺-binding domains, these undesirable characteristics are related to the RFP scaffold used to generate red GECIs.

To overcome the limitations associated with current RFP scaffolds, we turned our attention to the eqFP578-derived lineage of monomeric RFPs (i.e., mKate and its derivatives) [7,8,9], which tend to give bright and evenly distributed fluorescence when expressed in the neurons of transgenic mice [31]. Using a semi-rational design and directed evolution, we developed a new red fluorescent Ca²⁺ indicator, K-GECO1, based on the mKate variant FusionRed [9]. We anticipated that K-GECO1 would retain the favorable traits associated with its starting template RFP. We found this expectation to be generally true, as we did not observe lysosomal aggregation in dissociated rat neurons, zebrafish neurons, or fixed mouse brain tissue expressing K-GECO1. Some fluorescent punctate-like structures were observed during in vivo functional imaging.

The other distinctive feature of K-GECO1 is the use of the ckkap peptide as the CaM binding partner for the Ca²⁺-binding motif. Consistent with previous reports [23, 35], the ckkap/CaM motif yielded a lower apparent K_d for Ca²⁺ and faster kinetics (relative to RS20/CaM), and an apparent Hill coefficient close to 1. These characteristics should enable more sensitive detection of Ca²⁺ dynamics at physiological ranges, as is evident from K-GECO1’s large fluorescence response amplitude for a single action potential. With a Hill coefficient close to 1, K-GECO1 should provide a more linear Ca²⁺ response following multiple stimuli.

The X-ray crystal structure of K-GECO1 suggests that the indicator has a self-contained fluorescence modulation mechanism, similar to that proposed for R-GECO1 [22, 29]. Unlike GCaMP, in which the fluorescence modulation mechanism is dependent on the interactions with a residue of CaM [43] (Fig. 2l), the K-GECO1 Ca²⁺-bound state is likely stabilized by the hydrogen bonding between the phenolate group of chromophore and linker1 residue Asn32 (Fig. 2i). This makes the cpFusionRed protein in K-GECO1 a potentially useful template as a signal transduction domain to be combined with other binding domains for development of new types of red fluorescent indicators. The crystal structure also reveals that the ckkap/CaM motif in K-GECO1 has a reversed binding orientation for CaM compared with the RS20/CaM binding patterns in R-GECO1, RCaMP, and GCaMP6 (Fig. 2e–h). These results indicate that the GCaMP-like design is versatile enough to tolerate different peptide conformations and CaM orientations, and that exploring a wider range of CaM binding partners is likely to lead to GECIs with new and improved properties.

First-generation red GECIs, including mApple-based R-GECO1 and mRuby-based RCaMP1h, have been optimized using a neuron screening platform [24, 44], resulting in jRGECO1a and jRCaMP1a/b with greatly improved in vivo performance for detection of action potentials. Although K-GECO1 is a first-generation red GECI, it already provides performance that, by some criteria, is comparable to second-generation red GECIs. Specifically, K-GECO1 has a fluorescent response to single action potentials that is similar to that of jRGECO1a (and superior to jRCaMP1a/b) and faster dissociation kinetics than either jRGECO1a or jRCaMP1a/b. However, by other criteria, K-GECO1 will require further optimization to match the performance of second-generation red GECIs. For example, K-GECO1 does not provide the same level of in vivo sensitivity as the highly optimized jRGECO1a. In addition, K-GECO1 showed some blue-light-dependent photoactivation during in vitro characterization, though less so than R-GECO1. The photoactivation of K-GECO1 was not detectable under the illumination condition in our characterizations in cultured dissociated neurons (Additional file 5: Figure S3c) or in iPSC-CMs (Fig. 4c), suggesting that it is more suitable than R-GECO1 for use with blue/cyan-excitable optogenetic actuators. Nevertheless, the occurrence (or absence) of photoactivation will depend on the specific illumination conditions, and so appropriate controls (i.e., blue-light illumination of tissue expressing K-GECO1 but no optogenetic actuator) must be performed. Future efforts to screen K-GECO variants in neuronal cells, as was done for R-GECO1 and RCaMP1h [24], could lead to the discovery of improved variants with higher levels of expression in neurons, K_ds tuned to the range of neuronal cytoplasmic Ca²⁺ concentrations, increased cooperativity of Ca²⁺ binding to improve single action potential detection, diminished lysosomal accumulation, and minimum blue-light activation.

Conclusion

In summary, we have demonstrated the utility of K-GECO1 in various cell types including HeLa cells, dissociated neurons, iPSC-CMs, neurons in organotypic rat brain slices, zebrafish RB cells, and mouse V1 neurons in vivo. Though not yet ideal by all criteria, K-GECO1 represents a step forward in the development of red GECIs. Current users of red GECIs may find switching to K-GECO1 advantageous if their applications would benefit from faster kinetics, a more linear fluorescent response to multiple stimuli, or decreased photoactivation with blue-light illumination. For new users, we suggest performing initial trials with several different indicators to decide which one offers the best performance for their application. Due to the differences in expression and accumulation associated with red fluorescent proteins from different species and in different cellular contexts, new users should try one DsRed-derived GECI (e.g., jRGECO1a or R-CaMP2) [23, 24], one eqFP611-derived GECI (e.g., jRCaMP1a/b) [24], and one eqFP578-derived GECI (e.g., K-GECO1). As with R-GECO1 and RCaMP1h, further optimization using a neuron-based screening approach is likely to yield K-GECO variants with much improved sensitivity and performance in vivo.

Methods

Protein engineering

The design of K-GECO is based on well-established GECI designs reported previously [18, 33, 45,46,47]. The initial construction of mKate2 and FusionRed-based Ca²⁺ indicators was done by overlapping the assembly of the four DNA parts encoding the following protein fragments: the N-terminal (1–145) and C-terminal (146–223) parts of mKate2 or FusionRed, the RS20 peptide, and the CaM of R-GECO1. The fragments were amplified by PCR from mKate2, FusionRed (a kind gift from Michael Davidson), and R-GECO1 DNA. The overlap region and restriction sites were encoded in the primers. DNA encoding ckkap was synthesized by Integrated DNA Technologies (IDT). Purified PCR products were pooled and assembled in an overlapping PCR reaction. The resulting assembled PCR product was purified, digested with XhoI and HindIII (Thermo Fisher Scientific), and then ligated into a similarly digested pBAD/His B vector (Thermo Fisher Scientific). The ligation product was transformed into electrocompetent E. coli strain DH10B cells. Plasmids were purified with the GeneJET miniprep kit (Thermo Fisher Scientific) and then sequenced using the BigDye Terminator Cycle Sequencing kit (Thermo Fisher Scientific).

EP-PCR amplifications were performed to construct random mutagenesis libraries. The EP-PCR products were digested with XhoI and HindIII, and then ligated into a similarly digested pBAD/His B vector (Thermo Fisher Scientific). To construct site-directed mutagenesis and saturation mutagenesis libraries, QuikChange site-directed mutagenesis Lightning Single or Multi kit (Agilent Technologies) was used according to the manufacturer's instructions. The resulting variant libraries were transformed into electrocompetent E. coli strain DH10B cells and incubated overnight at 37 °C on 10-cm petri dishes with lysogeny broth (LB) agar supplemented with 400 g/mL ampicillin (Sigma) and 0.02% (wt/vol) L-arabinose (Alfa Aesar).

A custom imaging system was used for screening K-GECOs on plate with E. coli colonies expressing the variants [48]. When screening, fluorescence images of E. coli colonies were taken for each petri dish with an excitation filter of 542/27 nm and an emission filter of 609/57 nm. The colonies with the highest fluorescence intensity in each image were then picked and cultured in 4 mL liquid LB medium with 100 μg/ml ampicillin and 0.02% L-arabinose at 37 °C overnight. Proteins were then extracted using B-PER reagents (Thermo Fisher Scientific) from the liquid culture. The protein extraction was used for a secondary screen of the Ca²⁺-induced response test using Ca²⁺-free buffer (30 mM 3-(N-morpholino)propanesulfonic acid (MOPS), 100 mM KCl, and 10 mM EGTA at pH 7.2) and Ca²⁺-buffer (30 mM MOPS, 100 mM KCl, and 10 mM Ca-EGTA at pH 7.2) in a Safire2 fluorescence microplate reader (Tecan).

In vitro characterization

To purify K-GECO variants for in vitro characterization, the pBAD/His B plasmid encoding the variant of interest was used to transform electrocompetent E. coli DH10B cells and then plated on LB-agar plate with ampicillin (400 μg/mL). Single colonies were picked and inoculated into 5 mL LB medium supplemented with 100 g/mL ampicillin. Bacterial subcultures were incubated overnight at 37 °C. Then, 5 mL of bacterial subculture was added into 500 mL of LB medium with 100 μg/mL of ampicillin. The cultures were incubated at 37 °C to an OD of 0.6. Following induction with L-arabinose to a final concentration of 0.02% (wt/vol), the cultures were then incubated at 20 °C overnight. Bacteria were harvested by centrifugation at 4000 g for 10 min, resuspended in 30 mM Tris-HCl buffer (pH 7.4), lysed using a French press, and then clarified by centrifugation at 13,000 g for 30 mins. Proteins were purified from the cell-free extract by Ni-NTA affinity chromatography (MCLAB). The buffer of purified proteins was exchanged into 10 mM MOPS, 100 mM KCl, pH 7.2. Absorption spectra were recorded on a DU-800 UV-visible spectrophotometer (Beckman) and fluorescence spectra were recorded on a Safire2 fluorescence plate reader (Tecan).

To determine the quantum yield, the fluorescent protein mCherry was used as a standard. The detailed protocol has been described previously [18]. Briefly, the fluorescence emission spectra of each dilution of the protein solution of mCherry and K-GECO variants were recorded. The total fluorescence intensities were obtained by integration. The integrated fluorescence intensity versus absorbance was plotted for both mCherry and K-GECOs. The quantum yield was determined from the slopes of mCherry and K-GECOs. The extinction coefficient was determined by first measuring the absorption spectrum of K-GECO variants in a Ca²⁺-free buffer and a Ca²⁺-buffer. The absorption was measured following alkaline denaturation. The protein concentration was determined with the assumption that the denatured chromophore has an extinction coefficient of 44,000 M^-1 cm^-1 at 446 nm. The extinction coefficient of K-GECO variants was calculated by dividing the peak absorbance maximum by the concentration of protein.

For the Ca²⁺ K_d determination, the purified protein solution was diluted into a series of buffers, which were prepared by mixing Ca²⁺-buffer and Ca²⁺-free buffer with free Ca²⁺ concentration in a range from 0 to 3900 nM. The fluorescence intensity of K-GECO variants in each solution was measured and subsequently plotted as a function of Ca²⁺ concentration. The data were fitted to the Hill equation to obtain K_d and the apparent Hill coefficient.

Two-photon excitation spectra and cross sections were measured as previously reported [49], with the following adjustments. For the two-photon excited spectra (2PE), the fluorescence was collected through a 694/SP filter for K-GECO1 (Semrock). To correct for wavelength-to-wavelength variations in the laser parameters, a correction function using rhodamine B in MeOH and its known 2PE spectrum was applied [50]. Two-photon cross sections were measured at 1100 nm for K-GECO1, with rhodamine B in MeOH as a reference standard. The fluorescence for cross sections were collected through a narrow bandpass filter, 589/15 (Semrock), and differential quantum efficiencies were obtained at 582 nm with a PC1 ISS spectrofluorimeter (this wavelength corresponded to the bandpass center of the above filter when used in the MOM Sutter Instruments microscope due to its tilted position). Since the filter (694/SP) used for the 2PE spectra measurements covers the fluorescence of both the neutral and anionic forms of the chromophore, the spectrum of a particular Ca²⁺ state of a protein represents a combination of the unique 2PE spectra of the neutral and anionic forms, weighted to their relative concentrations (ρ, the concentration of one form divided by the total chromophore concentration) and quantum yields. The y-axis of the total 2PE spectrum is defined by F₂(λ) = σ₂,_N(λ) φ_N ρ_N + σ₂,_A(λ) φ_A ρ_A, where σ₂(λ) is the wavelength-dependent two-photon cross section and φ is the fluorescence quantum yield of the corresponding form (N for neutral or A for anionic in the subscript). At the wavelengths used to measure the cross sections (1060 and 1100 nm), σ₂,_N is assumed to be zero, and φ_A and ρ_A were independently measured to give a value for F₂ (Goeppert-Mayer, GM). The relative concentrations of the neutral and anionic forms were found by measuring the absolute extinction coefficients of each respective form in the Ca²⁺-free and the Ca²⁺-bound states. These differ from the effective extinction coefficients reported in Additional file 2: Table S1, which are weighted by the relative concentrations of both forms of the chromophore.

For the fluorescence correlation spectroscopy measurement of two-photon molecular brightness, dilute protein solutions (50–200 nM) in Ca²⁺ buffer (30 mM MOPS, 100 mM KCl, 10 mM CaEGTA, pH 7.2) were excited at 1060 nm at laser powers from 1 to 25 mW for 200 s. At each laser power, the fluorescence was recorded by an avalanche photodiode and fed to an Flex03LQ autocorrelator (Correlator.com). The measured autocorrelation curve was fitted to a simple diffusion model with a custom Matlab program [36] to determine the average number of excited molecules 〈N〉 in the excitation volume. The two-photon molecular brightness (ε) at each laser power was calculated as the average rate of fluorescence 〈F〉 per emitting molecule 〈N〉, defined as ε = 〈F〉/〈N〉 in kilocounts per second per molecule. As a function of laser power, the molecular brightness initially increases as the square of the laser power, then levels off and decreases due to photobleaching or saturation of the protein chromophore in the excitation volume. The maximum or peak brightness achieved, 〈e_max〉, represents a proxy for the photostability of a fluorophore.

To measure the photoswitching of K-GECO1, R-GECO1, and RCaMP1h in vitro, the purified protein in Ca²⁺ buffer (30 mM MOPS, 100 mM KCl, 10 mM CaEGTA, pH 7.2) or EGTA buffer (30 mM MOPS, 100 mM KCl, 10 mM EGTA, pH 7.2) were made into aqueous droplets with octanol in a 1:9 ratio and mounted on a presilanized coverslip. A single droplet was focused under the AxioImager microscope (Zeiss) with a 20× 0.8 NA objective and photoswitched by different laser excitations of 561, 405, and 488 nm. Fluorescence emission was detected using a SPCM-AQRH14 fiber coupled avalanche photodiode (Pacer).

Protein crystallography

K-GECO1 DNA was cloned into pRSET-A with a short N-terminal hexahistidine purification tag (MHHHHHHGSVKLIP…, tag underlined). K-GECO1 was expressed in T7 Express E. coli cells (New England Biolabs) for 36 h in autoinduction medium [51] supplemented with 100 mg/L ampicillin. E. coli pellets were lysed in B-PER (Thermo Fisher Scientific) supplemented with 1 mg/mL lysozyme followed by sonication. Insoluble cell debris was removed from the lysate by centrifugation for 20 min at 25,000 g, and soluble K-GECO1 protein was purified by immobilized metal affinity chromatography with nickel-charged Profinity resin (Bio-Rad), washed with 10 mM imidazole and eluted with 100 mM imidazole in Tris-buffered saline. K-GECO1 was further purified by size exclusion chromatography using a Superdex 200 column (GE Healthcare Life Sciences) with 10 mM Tris, 100 mM NaCl, pH 8.0, as the mobile phase. Purified K-GECO was concentrated to 10 mg/mL for crystallization using centrifugal concentrators (Sartorius Vivaspin, 10,000 molecular weight cut-off (MWCO)). Purified K-GECO1 protein at 10 mg/mL in 10 mM Tris, 100 mM NaCl, pH 8.0, was mixed with an equal volume of a precipitant solution containing 100 mM BIS-TRIS, 20% w/v polyethylene glycol monomethyl ether 5000, pH 6.5, at room temperature in a sitting-drop vapor diffusion crystallization tray (Hampton Research). Crystals were cryoprotected in the precipitant solution supplemented with 25% ethylene glycol. X-ray diffraction data were collected at 100 K on beamline 8.2.1 of the Advanced Light Source. Diffraction data were processed using the HKL software package [52]. The structure was solved by molecular replacement using Phaser [53], searching first for two copies of the fluorescent protein domain fragment using a single molecule of mKate (PDB ID 3BXB) as the search model, followed by two copies each of the separated N- and C-terminal lobes of the Ca²⁺-bound calmodulin domain using fragments of PDB ID 3SG3. Iterative model building in Coot [54] and refinement in Refmac [55] produced the K-GECO1 model, with two copies of K-GECO1 in the asymmetric unit. The K-GECO1 model was deposited at the PDB with the accession code 5UKG.

Cell culture and imaging

To characterize the K-GECO variants in HeLa cells, the cells were maintained in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), penicillin-streptomycin (Thermo Fisher Scientific), GlutaMAX (Thermo Fisher Scientific) at 37 °C with 5% CO₂. To construct the mammalian expression plasmid, pcDNA3.1(+) and the K-GECO variant were both digested with XhoI and HindIII, and the digested plasmid backbone and insert were purified by gel electrophoresis, followed by ligation and sequencing confirmation. Transient transfections of pcDNA3.1(+)-K-GECO plasmids were performed using Lipofectamine 2000 (Thermo Fisher Scientific). HeLa cells (60–70% confluency) on 35 mm glass bottom dishes (In vitro Scientific) were transfected with 1 μg of plasmid DNA, using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions. The cells were imaged 24 h after the transfection. Immediately prior to imaging, cells were washed twice with Hanks balanced salt solution (HBSS) and then 1 mL of 20 mM HEPES buffered HBSS (HHBSS) was added. Cell imaging was performed with an inverted Eclipse Ti (Nikon). The AquaCosmos software package (Hamamatsu) was used for automated microscope and camera control. Cells were imaged with a 20× objective lens. To image the histamine-induced Ca²⁺ dynamics, cells were imaged with a 200 ms exposure acquired every 5 s for a duration of 30 min. Approximately 60 s after the start of the experiment, histamine (10 μL) was added to a final concentration of 5 mM. The oscillation was imaged for 20 min, EGTA/ionomycin (40 μL) in HHBSS was added to a final concentration of 2 mM EGTA and 5 μM of ionomycin. After 5 min, Ca²⁺/ionomycin (40 μL) in Ca²⁺ and Mg²⁺-free HHBSS was added to a final concentration of 5 mM Ca²⁺ and 5 μM of ionomycin.

To characterize K-GECO variants in cultured dissociated neurons, the procedure was done as previously reported [29]. Dissociated E18 Sprague–Dawley hippocampal cells were purchased from BrainBits LLC. The cells were grown on a 35-mm glass-bottomed dish (In Vitro Scientific) containing NbActiv4 medium (BrainBits LLC) supplemented with 2% FBS, penicillin-G potassium salt (50 units/ml), and streptomycin sulfate (50 mg/ml). Half of the culture media was replaced every 4 or 5 days. Cells were transfected on day 8 using Lipofectamine 2000 (Thermo Fisher Scientific) following the manufacturer’s instructions with the following modifications. Briefly, 1–2 μg of plasmid DNA and 4 μl of Lipofectamine 2000 (Thermo Fisher Scientific) were added to 100 μl of NbActive4 medium to make the transfection medium and incubated at room temperature for 10–15 min. Half of the culture medium (1 ml) from each neuron dish was taken out and combined with an equal volume of fresh NbActiv4 medium (supplemented with 2% FBS, penicillin-G potassium salt, and streptomycin sulfate) to make a 1:1 mixture and incubated at 37 °C and 5% CO₂. Then, 1 ml of fresh conditioned (at 37 °C and 5% CO₂) NbActiv4 medium was added to each neuron dish. After the addition of transfection medium, the neuron dishes were incubated for 2–3 h at 37 °C in a CO₂ incubator. The medium was then replaced using the conditioned 1:1 mixture medium prepared previously. The cells were then incubated for 48–72 h at 37 °C in a CO₂ incubator before imaging. Fluorescence imaging was performed in HHBSS on an inverted Nikon Eclipse Ti-E microscope equipped with a 200 W metal halide lamp (PRIOR Lumen), 60× oil objectives (numerical aperture, NA = 1.4; Nikon), a 16-bit QuantEM 512SC electron-multiplying CCD camera (Photometrics), and a TRITC/Cy3 filter set (545/30 nm excitation, 620/60 nm emission, and a 570LP dichroic mirror, Chroma). For time-lapse imaging, neurons were imaged at an imaging frequency of 100 Hz with 4 × 4 binning. For photoactivation comparison, cells expressing K-GECO1 and R-GECO1 were stimulated with pulses of blue laser light (405 nm, 5 mW/mm²).

To compare the K-GECO1 and red GECIs in stimulated cultured neuron cells, the procedure was done as previously reported [24]. Briefly, red GECIs were expressed after electroporation into rat primary hippocampal neurons (P0) using the Nucleofector system (Lonza). For stimulation, action potentials were evoked by field stimulation. The TxRed filter set (540–580 nm excitation, 593–668 nm emission, and 585-nm-long pass dichroic mirror) was used for illumination. Responses were quantified for each cell as the change in fluorescence divided by the baseline fluorescence before stimulation. The signal-to-noise ratio was quantified as the peak fluorescence signal over the baseline, divided by the standard deviation of the fluorescence signal before the stimulation.

iPSC-CMs were purchased from Axol Bioscience. Cells were plated in two wells of a six-well plate and cultured for 4 days in Cardiomyocyte Maintenance Medium (Axol Bioscience) to 60–80% confluency. Cells then were then transferred to fibronectin-coated (1%) coverslips and imaged in Tyrode’s buffer. Cells were transfected using transfection reagent Lipofectamine 2000 (Invitrogen). An inverted microscope (Zeiss) equipped with a NA 1.4, 63× objective lens (Zeiss) and a pE-4000 multi-wavelength LED light source (CoolLED) was used. Blue (470 nm) and green (550 nm) excitation were used to illuminate ChR2-EYFP and red GECIs, respectively. The green fluorescent protein filter set (excitation 480/10 nm, 495 nm long pass dichroic mirror, emission 525/50 nm) and the RFP filter set (excitation 545/30, 565 nm long pass dichroic mirror, emission 620/60 nm) were used to visualize ChR2-EYFP and K-GECO or R-GECO, respectively. Optical stimulation was achieved with the 470-nm LED light at a power density of 0.19 W/cm² and a pulse duration of 150 ms. Fluorescence signals were recorded using an ORCA-Flash4.0LT sCMOS camera (Hamamatsu) controlled by ImageJ [56].

Organotypic hypothalamic rat brain slice imaging

To prepare organotypic brain slices, experiments were done on neonatal rat coronal brain slices containing the VMN of the hypothalamus. In brief, postnatal 0–1-day-old Sprague–Dawley rats were anesthetized with 2–3% isoflurane until the paw reflex disappeared. Following decerebration, the brain was isolated in ice-cold divalent cation-free HBSS (Thermo Fisher Scientific) with 1 mM CaCl₂ and 1.3 mM MgSO₄. The brain was glued caudal side down to a metal plate and serial sections of 400 μm thickness were made using a vibratome (Leica Microsystems). Sectioning was stopped when the third ventricle became visible and two VMN-containing slices of 250 μm thickness were cut. Individual slices were placed on a sterile 0.4-μm-pore-membrane cell culture insert (Millipore). The insert and slice were then transferred to a 35-mm-diameter culture dish (Corning) containing 1.5 ml of NbActiv4 medium (BrainBits) supplemented with 5% FBS, penicillin-G potassium salt (50 units/ml), and streptomycin sulfate (50 μg/ml). Slices were cultured at 37 °C in an incubator (Thermo Fisher Scientific) under gassing with 5% CO₂.

For transfection of organotypic slices, after 8–10 days of organotypic slice culturing, the VMN areas were transfected with an electroporation technique as previously described [47]. Specifically, the insert with the slice was placed on a platinum plate petri dish electrode (Bex Co Ltd) and electroporation buffer (HBSS with 1.5 mM MgCl₂ and 10 mM D-glucose) was filled between the electrode and the membrane. Plasmids of pcDNA3.1-K-GECO1 were dissolved in the electroporation buffer at a concentration of 1 μg/ml and 10 μl of this solution was added to just cover the slice. Then, a square platinum electrode (Bex Co Ltd) was placed directly above the slice. Five 25-V pulses (5 ms duration and interval 1 s) were applied twice (the second time with reversed polarity) using a pulse stimulator (Sequim) and an amplifier (Agilent). The electroporation buffer was replaced with supplemented NbActiv4 medium and slices were returned to the incubator.

To image the cytosolic Ca²⁺ dynamics using K-GECO1, an upright FV1000 confocal microscope equipped with FluoView software and a 20× XLUMPlanF1 water immersion objective (NA 1.0) was used (Olympus). The Millipore insert containing a transfected brain slice was placed in a custom-made chamber and mechanically fixed with a platinum harp. The slices were then perfused at 31 °C with artificial cerebrospinal fluid containing (in mM) 120 NaCl, 3 KCl, 1 CaCl₂, 1.3 MgSO₄, 26 NaHCO₃, 1.25 NaH₂PO₄, and 10 D-glucose (the pH was adjusted to 7.4 by gassing with 95% O₂ plus 5% CO₂), at a flow rate of 5 ml/min using a peristaltic pump (Watson-Marlow). For single-color confocal Ca_i imaging, K-GECO-transfected VMN neurons were exposed to excitation with 543-nm laser light and emissions were collected from 560 to 660 nm using a variable barrier filter. Images were acquired at × 1–3 digital zoom at a frame resolution of 512 × 512 and with a 2 μs/pixel scanning rate resulting in image acquisition at 1.12 frames/s. To monitor the drug-evoked cytosolic Ca²⁺ rises approximately 60 s after the start of image acquisition, 100 μM ATP (Sigma-Aldrich) was added to the artificial cerebrospinal fluid for 90 s. To compare the K-GECO1 signal with that of a chemical Ca²⁺ fluorescent dye, transfected slices were stained with the membrane-permeant (AM) variant of green Fluo-4 by focal application. In brief, 0.5 mM of Fluo-4-AM was filled into a broken patch pipette with an outer diameter of ~10 μm and subsequently pressure-injected (25–50 mmHg) for 10 min [57, 58] at 30–50 μm depth into the slice in the vicinity of the K-GECO1-transfected VMN neurons. This led to the uniform staining of cells in a radius of 150–200 μm from the injection site. For dual-color imaging of K-GECO1- and Fluo-4-based Ca²⁺ responses, double-labeled neurons were excited with a 488-nm laser and emissions were simultaneously collected in two channels from 500 to 520 nm for Fluo-4 and 570 to 670 nm for K-GECO1 using variable barrier filters.

Imaging of zebrafish spinal sensory neurons

Mitfa^w2/w2 roy^a9/a9 (Casper) zebrafish were maintained under standard conditions at 28 °C and a 14:10 hr light:dark cycle. Embryos (cell stage 1–2) of Tg (elavl3:GAL4-VP16) [59] were injected with 25 ng/μl DNA plasmids encoding the K-GECO variants under the control of the 10xUAS promoter, and 25 ng/μL Tol2 transposase mRNA diluted in E3 medium. Three-day post-fertilization embryos showing expression in spinal sensory neurons (RB cells) were paralyzed by a 5-min bath application of 1 mg/ml a-bungarotoxin (Sigma, 203980). Larvae were mounted on their side in a field stimulation chamber (Warner, RC-27NE2) with 1.5% low-melting-point agarose and imaged using a custom-built two-photon microscope equipped with a resonant scanner. The light source was an Insight DS Dual femtosecond laser (Spectra-Physics) running at 1140 nm. The objective was a 25× 0.95 NA water immersion lens (Leica). Functional images (512 × 256 pixels) were acquired using ScanImage 5 (vidriotechnologies.com) at 7.5 Hz. The approximate laser power at the sample was measured using a slide power meter (Thorlabs) and 3 and 20 mW were used for functional imaging. Trains of 1, 2, 5, 10, and 20 field stimuli (1 ms pulse width at 50 Hz) were applied with a stimulator (NPI ISO-STIM). The stimulation voltage was calibrated to elicit an identifiable response to a single pulse in RB cells without stimulating muscle cells. Regions of interest (ROIs) were selected manually, and data were analyzed using MATLAB (MathWorks).

Mouse V1 imaging

For in vivo mouse V1 imaging, the procedure was done as previously reported [24]. Briefly, AAV injection was used for expression of K-GECO1 in mouse V1 neurons. After injection of the virus, a cranial window was implanted. The animal was then placed under a microscope at 37 °C and anesthetized during imaging. A custom-built two-photon microscope was used for imaging with a 1100-nm pulse laser as light source and a 16× 0.8 NA water immersion lens as objective. The laser power was 100–150 mW at the front aperture of the objective lens. The moving grating stimulus trial consisted of a blank period followed by a drifting sinusoidal grating with eight drifting directions with 45° separation. The gratings were presented with an LCD screen placed in front of the center of the right eye of the mouse. For the fixed tissue analysis, the mice were anesthetized and transcardially perfused. The brains were then removed and post-fixed. Sections of the brains were coverslipped and imaged using confocal microscopy (LSM 710, Zeiss).

Statistical analysis

All data are expressed as means ± standard deviation. Sample sizes (n) are listed for each experiment. For V1 functional imaging, the ANOVA test (p = 0.01) was used to identify responsive cells for each of the grating stimuli.

Change history

30 October 2019
In the online version of the article [1], Figure S1 was mistakenly replaced with Figure 1.

References

Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA, et al. A monomeric red fluorescent protein. Proc Natl Acad Sci U S A. 2002;99(12):7877–82.
Article CAS PubMed PubMed Central Google Scholar
Shaner NC, Campbell RE, Steinbach PA, Giepmans BNG, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004;22(12):1567–72.
Article CAS PubMed Google Scholar
Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson MW, et al. Improving the photostability of bright monomeric orange and red fluorescent proteins. Nat Methods. 2008;5(6):545–51.
Article CAS PubMed PubMed Central Google Scholar
Shen Y, Chen Y, Wu J, Shaner NC, Campbell RE. Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity. PLoS One. 2017;12(2):e0171257.
Article PubMed PubMed Central CAS Google Scholar
Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, et al. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods. 2007;4(7):555–7.
Article CAS PubMed Google Scholar
Wiedenmann J, Schenk A, Röcker C, Girod A, Spindler K-D, Nienhaus GU. A far-red fluorescent protein with fast maturation and reduced oligomerization tendency from Entacmaea quadricolor (Anthozoa, Actinaria). Proc Natl Acad Sci U S A. 2002;99(18):11646–51.
Article CAS PubMed PubMed Central Google Scholar
Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, et al. Bright far-red fluorescent protein for whole-body imaging. Nat Methods. 2007;4(9):741–6.
Article CAS PubMed Google Scholar
Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, et al. Far-red fluorescent tags for protein imaging in living tissues. Biochem J. 2009;418(3):567–74.
Article CAS PubMed Google Scholar
Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, Souslova EA, et al. A monomeric red fluorescent protein with low cytotoxicity. Nat Commun. 2012;3:1204.
Article CAS PubMed Google Scholar
Kredel S, Oswald F, Nienhaus K, Deuschle K, Röcker C, Wolff M, et al. mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures. PLoS One. 2009;4(2):e4391.
Article PubMed PubMed Central CAS Google Scholar
Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, et al. Improving FRET dynamic range with bright green and red fluorescent proteins. Nat Methods. 2012;9(10):1005–12.
Article CAS PubMed PubMed Central Google Scholar
Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, et al. Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Sci Rep. 2016;6:20889.
Article CAS PubMed PubMed Central Google Scholar
Shen Y, Lai T, Campbell RE. Red fluorescent proteins (RFPs) and RFP-based biosensors for neuronal imaging applications. Neurophotonics. 2015;2(3):031203.
Article PubMed PubMed Central Google Scholar
Kim TH, Zhang Y, Lecoq J, Jung JC, Li J, Zeng H, et al. Long-term optical access to an estimated one million neurons in the live mouse cortex. Cell Rep. 2016;17(12):3385–94.
Article CAS PubMed PubMed Central Google Scholar
Tian L, Hires SA, Mao T, Huber D, Chiappe ME, Chalasani SH, et al. Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators. Nat Methods. 2009;6(12):875–81.
Article CAS PubMed PubMed Central Google Scholar
Akerboom J, Chen T-W, Wardill TJ, Tian L, Marvin JS, Mutlu S, et al. Optimization of a GCaMP calcium indicator for neural activity imaging. J Neurosci. 2012;32(40):13819–40.
Article CAS PubMed PubMed Central Google Scholar
Chen T-W, Wardill TJ, Sun Y, Pulver SR, Renninger SL, Baohan A, et al. Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 2013;499(7458):295–300.
Article CAS PubMed PubMed Central Google Scholar
Zhao Y, Araki S, Wu J, Teramoto T, Chang Y-F, Nakano M, et al. An expanded palette of genetically encoded Ca²⁺ indicators. Science. 2011;333(6051):1888–91.
Article CAS PubMed PubMed Central Google Scholar
Akerboom J, Carreras Calderón N, Tian L, Wabnig S, Prigge M, Tolö J, et al. Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics. Front Mol Neurosci. 2013;6:2.
Article CAS PubMed PubMed Central Google Scholar
Ohkura M, Sasaki T, Kobayashi C, Ikegaya Y, Nakai J. An improved genetically encoded red fluorescent Ca²⁺ indicator for detecting optically evoked action potentials. PLoS One. 2012;7(7):e39933.
Article CAS PubMed PubMed Central Google Scholar
Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, et al. Improved orange and red Ca²⁺ indicators and photophysical considerations for optogenetic applications. ACS Chem Neurosci. 2013;4(6):963–72.
Article CAS PubMed PubMed Central Google Scholar
Wu J, Abdelfattah AS, Miraucourt LS, Kutsarova E, Ruangkittisakul A, Zhou H, et al. A long Stokes shift red fluorescent Ca²⁺ indicator protein for two-photon and ratiometric imaging. Nat Commun. 2014;5:5262.
Article CAS PubMed Google Scholar
Inoue M, Takeuchi A, Horigane S-I, Ohkura M, Gengyo-Ando K, Fujii H, et al. Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. Nat Methods. 2014;12(1):64–70.
Article PubMed CAS Google Scholar
Dana H, Mohar B, Sun Y, Narayan S, Gordus A, Hasseman JP, et al. Sensitive red protein calcium indicators for imaging neural activity. Elife. 2016;5:e12727.
Article PubMed PubMed Central CAS Google Scholar
Shen Y, Wiens MD, Campbell RE. A photochromic and thermochromic fluorescent protein. RSC Adv. 2014;4(100):56762–5.
Article CAS Google Scholar
Shen Y, Rosendale M, Campbell RE, Perrais D. pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis. J Cell Biol. 2014;207(3):419–32.
Article CAS PubMed PubMed Central Google Scholar
Yamada Y, Mikoshiba K. Quantitative comparison of novel GCaMP-type genetically encoded Ca²⁺ indicators in mammalian neurons. Front Cell Neurosci. 2012;6:1–9.
Article Google Scholar
Han L, Zhao Y, Zhang X, Peng J, Xu P, Huan S, et al. RFP tags for labeling secretory pathway proteins. Biochem Biophys Res Commun. 2014;447(3):508–12.
Article CAS PubMed Google Scholar
Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul A, et al. A bright and fast red fluorescent protein voltage indicator that reports neuronal activity in organotypic brain slices. J Neurosci. 2016;36(8):2458–72.
Article CAS PubMed PubMed Central Google Scholar
Bethge P, Carta S, Lorenzo DA, Egolf L, Goniotaki D, Madisen L, et al. An R-CaMP1.07 reporter mouse for cell-type-specific expression of a sensitive red fluorescent calcium indicator. PLoS One. 2017;12(6):e0179460.
Article PubMed PubMed Central CAS Google Scholar
Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR. Improved tools for the Brainbow toolbox. Nat Methods. 2013;10(6):540–7.
Article CAS PubMed PubMed Central Google Scholar
Pletnev S, Shcherbo D, Chudakov DM, Pletneva N, Merzlyak EM, Wlodawer A, et al. A crystallographic study of bright far-red fluorescent protein mKate reveals pH-induced cis-trans isomerization of the chromophore. J Biol Chem. 2008;283(43):28980–7.
Article CAS PubMed PubMed Central Google Scholar
Nakai J, Ohkura M, Imoto K. A high signal-to-noise Ca²⁺ probe composed of a single green fluorescent protein. Nat Biotechnol. 2001;19(2):137–41.
Article CAS PubMed Google Scholar
Shui B, Wang Q, Lee F, Byrnes LJ, Chudakov DM, Lukyanov SA, et al. Circular permutation of red fluorescent proteins. PLoS One. 2011;6(5):e20505.
Article CAS PubMed PubMed Central Google Scholar
Truong K, Sawano A, Mizuno H, Hama H, Tong KI, Mal TK, et al. FRET-based in vivo Ca²⁺ imaging by a new calmodulin-GFP fusion molecule. Nat Struct Biol. 2001;8(12):1069–73.
Article CAS PubMed Google Scholar
Mütze J, Iyer V, Macklin JJ, Colonell J, Karsh B, Petrášek Z, et al. Excitation spectra and brightness optimization of two-photon excited probes. Biophys J. 2012;102(4):934–44.
Article PubMed PubMed Central CAS Google Scholar
Kurokawa H, Osawa M, Kurihara H, Katayama N, Tokumitsu H, Swindells MB, et al. Target-induced conformational adaptation of calmodulin revealed by the crystal structure of a complex with nematode Ca²⁺/calmodulin-dependent kinase kinase peptide. J Mol Biol. 2001;312(1):59–68.
Article CAS PubMed Google Scholar
Tidow H, Nissen P. Structural diversity of calmodulin binding to its target sites. FEBS J. 2013;280(21):5551–65.
Article CAS PubMed Google Scholar
Palmer AE, Tsien RY. Measuring calcium signaling using genetically targetable fluorescent indicators. Nat Protoc. 2006;1(3):1057–65.
Article CAS PubMed Google Scholar
Chen ZP, Levy A, Lightman SL. Activation of specific ATP receptors induces a rapid increase in intracellular calcium ions in rat hypothalamic neurons. Brain Res. 1994;641(2):249–56.
Article CAS PubMed Google Scholar
Higashijima S-I, Masino MA, Mandel G, Fetcho JR. Imaging neuronal activity during zebrafish behavior with a genetically encoded calcium indicator. J Neurophysiol. 2003;90(6):3986–97.
Article PubMed Google Scholar
Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K. Millisecond-timescale, genetically targeted optical control of neural activity. Nat Neurosci. 2005;8(9):1263–8.
Article CAS PubMed Google Scholar
Akerboom J, Rivera JDV, Guilbe MMR, Malavé ECA, Hernandez HH, Tian L, et al. Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J Biol Chem. 2009;284(10):6455–64.
Article CAS PubMed PubMed Central Google Scholar
Wardill TJ, Chen T-W, Schreiter ER, Hasseman JP, Tsegaye G, Fosque BF, et al. A neuron-based screening platform for optimizing genetically-encoded calcium indicators. PLoS One. 2013;8(10):e77728.
Article CAS PubMed PubMed Central Google Scholar
Baird GS, Zacharias DA, Tsien RY. Circular permutation and receptor insertion within green fluorescent proteins. Proc Natl Acad Sci U S A. 1999;96(20):11241–6.
Article CAS PubMed PubMed Central Google Scholar
Nagai T, Sawano A, Park ES, Miyawaki A. Circularly permuted green fluorescent proteins engineered to sense Ca²⁺. Proc Natl Acad Sci U S A. 2001;98(6):3197–202.
Article CAS PubMed PubMed Central Google Scholar
Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, Campbell RE, et al. Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response. Integr Biol. 2014;6(7):714–25.
Article CAS Google Scholar
Ai H-W, Baird MA, Shen Y, Davidson MW, Campbell RE. Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications. Nat Protoc. 2014;9(4):910–28.
Article CAS PubMed Google Scholar
Barnett LM, Hughes TE, Drobizhev M. Deciphering the molecular mechanism responsible for GCaMP6m’s Ca²⁺-dependent change in fluorescence. PLoS One. 2017;12(2):e0170934.
Article PubMed PubMed Central CAS Google Scholar
Makarov NS, Drobizhev M, Rebane A. Two-photon absorption standards in the 550–1600 nm excitation wavelength range. Opt Express. 2008;16(6):4029–47.
Article CAS PubMed Google Scholar
Studier FW. Protein production by auto-induction in high density shaking cultures. Protein Expr Purif. 2005;41(1):207–34.
Article CAS PubMed Google Scholar
Otwinowski Z, Minor W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 1997;276:307–26.
Article CAS PubMed Google Scholar
McCoy AJ, Grosse-Kunstleve RW, Adams PD, Winn MD, Storoni LC, Read RJ. Phaser crystallographic software. J Appl Crystallogr. 2007;40(Pt 4):658–74.
Article CAS PubMed PubMed Central Google Scholar
Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot. Acta Crystallogr D Biol Crystallogr. 2010;66(Pt 4):486–501.
Article CAS PubMed PubMed Central Google Scholar
Winn MD, Ballard CC, Cowtan KD, Dodson EJ, Emsley P, Evans PR, et al. Overview of the CCP4 suite and current developments. Acta Crystallogr D Biol Crystallogr. 2011;67(Pt 4):235–42.
Article CAS PubMed PubMed Central Google Scholar
Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 2012;9(7):671–5.
Article CAS PubMed PubMed Central Google Scholar
Ruangkittisakul A, Schwarzacher SW, Secchia L, Poon BY, Ma Y, Funk GD, et al. High sensitivity to neuromodulator-activated signaling pathways at physiological [K+] of confocally imaged respiratory center neurons in on-line-calibrated newborn rat brainstem slices. J Neurosci. 2006;26(46):11870–80.
Article CAS PubMed PubMed Central Google Scholar
Ruangkittisakul A, Schwarzacher SW, Secchia L, Ma Y, Bobocea N, Poon BY, et al. Generation of eupnea and sighs by a spatiochemically organized inspiratory network. J Neurosci. 2008;28(10):2447–58.
Article CAS PubMed PubMed Central Google Scholar
Kimura Y, Satou C, Higashijima S-I. V2a and V2b neurons are generated by the final divisions of pair-producing progenitors in the zebrafish spinal cord. Development. 2008;135(18):3001–5.
Article CAS PubMed Google Scholar
Ding J, Luo AF, Hu L, Wang D, Shao F. Structural basis of the ultrasensitive calcium indicator GCaMP6. Sci China Life Sci. 2014;57(3):269–74.
Article CAS PubMed Google Scholar

Download references

Acknowledgements

We thank the University of Alberta Molecular Biology Services Unit for technical support and Christopher W. Cairo for providing access to instrumentation.

Funding

YS was supported by an Alberta Innovate Scholarship. ASA was supported by a Vanier Canada Graduate Scholarship and an Alberta Innovates Health Solutions studentship. BR was supported by the Natural Sciences and Engineering Research Council (NSERC; RGPIN-2014-06484). KB was supported by the University Hospital Foundation. Work in the lab of REC is supported by grants from Canadian Institutes of Health Research (MOP-123514), NSERC (RGPIN 288338-2010), Brain Canada, and the National Institutes of Health (U01 NS094246 and UO1 NS090565).

Availability of data and materials

All source data are available in the supplementary information files associated with the manuscript. Plasmids encoding K-GECO1 are available through Addgene and from the corresponding author upon reasonable request. Correspondence and requests for materials should be addressed to REC (robert.e.campbell@ualberta.ca). Supporting numeric data are provided in Additional files 8, 9, 10, 11, 12, 13 and 14.

Author information

Authors and Affiliations

Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2, Canada
Yi Shen, Ahmed S. Abdelfattah, Lanshi Wu, Yingche Chen, Yong Qian, Matthew D. Wiens, Nathan Hambleton & Robert E. Campbell
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA
Hod Dana, Ronak Patel, Jamien Shea, Douglas S. Kim, Minoru Koyama & Eric R. Schreiter
Department of Cell Biology and Neuroscience, Montana State University, Bozeman, MT, 59717, USA
Rosana S. Molina, Thomas E. Hughes & Mikhail Drobizhev
Department of Physiology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada
Bijal Rawal, Vladimir Rancic & Klaus Ballanyi
LumiSTAR Biotechnology Incorporation, Nangang District, Taipei City, 115, Taiwan
Yu-Fen Chang
Present address: Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, 4195, USA
Hod Dana
Present address: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA
Ahmed S. Abdelfattah

Authors

Yi Shen
View author publications
You can also search for this author in PubMed Google Scholar
Hod Dana
View author publications
You can also search for this author in PubMed Google Scholar
Ahmed S. Abdelfattah
View author publications
You can also search for this author in PubMed Google Scholar
Ronak Patel
View author publications
You can also search for this author in PubMed Google Scholar
Jamien Shea
View author publications
You can also search for this author in PubMed Google Scholar
Rosana S. Molina
View author publications
You can also search for this author in PubMed Google Scholar
Bijal Rawal
View author publications
You can also search for this author in PubMed Google Scholar
Vladimir Rancic
View author publications
You can also search for this author in PubMed Google Scholar
Yu-Fen Chang
View author publications
You can also search for this author in PubMed Google Scholar
Lanshi Wu
View author publications
You can also search for this author in PubMed Google Scholar
Yingche Chen
View author publications
You can also search for this author in PubMed Google Scholar
Yong Qian
View author publications
You can also search for this author in PubMed Google Scholar
Matthew D. Wiens
View author publications
You can also search for this author in PubMed Google Scholar
Nathan Hambleton
View author publications
You can also search for this author in PubMed Google Scholar
Klaus Ballanyi
View author publications
You can also search for this author in PubMed Google Scholar
Thomas E. Hughes
View author publications
You can also search for this author in PubMed Google Scholar
Mikhail Drobizhev
View author publications
You can also search for this author in PubMed Google Scholar
Douglas S. Kim
View author publications
You can also search for this author in PubMed Google Scholar
Minoru Koyama
View author publications
You can also search for this author in PubMed Google Scholar
Eric R. Schreiter
View author publications
You can also search for this author in PubMed Google Scholar
Robert E. Campbell
View author publications
You can also search for this author in PubMed Google Scholar

Contributions

YS, LW, and MDW performed the rational design and directed evolution of K-GECO variants. YS, YQ, and RP performed in vitro one-photon characterization. RSM, RP, and MD performed in vitro two-photon characterization. ERS crystallized the protein and solved the structure. YC performed imaging of HeLa cells. HD, ASA, NH, and VR performed imaging of dissociated neurons. YFC performed imaging of iPSC-CMs. BR and VR performed imaging of organotypic slices. JS and MK performed in vivo imaging of zebrafish. HD performed in vivo imaging of mice. REC, ERS, DSK, KB, and TEH supervised the research. YS and REC wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Robert E. Campbell.

Ethics declarations

Ethics approval

All procedures for organotypic slices were carried out in compliance with the guidelines of the Canadian Council for Animal Care and with the approval of the University of Alberta Health Animal Care and Use Committee for Health Sciences. All experimental protocols for mouse and zebrafish in vivo imaging were conducted according to National Institutes of Health guidelines for animal research and were approved by the Institutional Animal Care and Use Committee at Janelia Research Campus (protocol 13-95).

Competing interests

YFC is the founder of a company that will commercialize K-GECO1 as reported in this work. The other authors have declared that no competing interests exist.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Additional files

Additional file 1: Figure S1.

Protein sequence alignment of K-GECO1, R-CaMP2, R-GECO1, and RCaMP1h. Reserved residues are colored in blue. Different residues are highlighted in red. Structural information is indicated with colored bars below the aligned sequences. (TIF 675 kb)

Additional file 2: Table S1.

In vitro photophysical characteristics of K-GECO1, R-GECO1, and RCaMP1h (-/+ Ca²⁺) (DOC 34 kb)

Additional file 3: Figure S2.

In vitro photoactivation characterization of K-GECO1, R-GECO1, and RCaMP1h. a Representative K-GECO1 fluorescence response to switching between 4 s of illumination with a 561-nm (6.13 W/cm²) laser and 1 s with a 405- nm (1.76 W/cm²) or 488-nm (6.13 W/cm²) laser in the presence and absence (EGTA buffer) of Ca²⁺. b Representative R-GECO1 fluorescence response with switching between 4 s of a 561-nm (3.83 W/cm²) laser and 1 s of a 405-nm (0.08 W/cm²) or 488-nm (3.83 W/cm²) laser in both Ca²⁺ buffer and Ca²⁺-free buffer. c Representative RCaMP1h fluorescence response with switching between 4 s of a 561-nm (3.83 W/cm²) laser and 1 s of a 405-nm (0.08 W/cm²) or a 488-nm (3.83 W/cm²) laser in both Ca²⁺ buffer and Ca²⁺-free buffer. d Percentage fluorescence change of K-GECO1, R-GECO1, and RCaMP1h in Ca²⁺-free buffer after applying 1 s of a 488-nm laser with various intensities when illuminated with a 561-nm laser (n = 5 photoswitching cycles for K-GECO1; n = 6–9 photoswitching cycles for R-GECO1; n = 6 photoswitching cycles for RCaMP1h). Supporting numeric data are provided in Additional file 13. (TIF 1097 kb)

Additional file 4: Table S2.

X-ray diffraction data collection and model refinement statistics. (DOC 48 kb)

Additional file 5: Figure S3.

Fluorescence localization and photoactivation of K-GECO1 and R-GECO1 in cultured neurons. a Representative fluorescence image of a K-GECO1-transfected cultured hippocampal neuron. b Representative fluorescence image of a R-GECO1-transfected cultured hippocampal neuron. Fluorescent puncta structures are indicated by the arrowhead. c K-GECO1 fluorescence response in neurons when applying 405-nm laser illumination. d R-GECO1 fluorescence response in neurons when applying 405-nm laser illumination. Supporting numeric data are provided in Additional file 14. (TIF 480 kb)

Additional file 6: Figure S4.

K-GECO1 expression patterns in zebrafish Rohon–Beard (RB) cells. a Schematic view of the image window. b Representative images of K-GECO1 expression in RB cells. c Representative images of jRGECO1a (with NES) expression in RB cells. (TIF 1782 kb)

Additional file 7: Figure S5.

K-GECO1 expression patterns in mouse V1 neurons. a Representative images of K-GECO1 (with NES) expression in a fixed tissue section from a mouse V1. b Representative image and zoom-in view of K-GECO1 expression in functional imaging of mouse V1 neurons. (TIF 2598 kb)

Additional file 8:

Numeric data for Fig. 2. (XLSX 25 kb)

Additional file 9:

Numeric data for Fig. 3a,b. (XLSX 86 kb)

Additional file 10:

Numeric data for Fig. 4. (XLSX 123 kb)

Additional file 11:

Numeric data for Fig. 5. (XLSX 101 kb)

Additional file 12:

Numeric data for Fig. 6e–h. (XLSX 13 kb)

Additional file 13:

Numeric data for Additional file 3: Figure S2. (XLSX 259 kb)

Additional file 14:

Numeric data for Additional file 5: Figure S3. (XLSX 88 kb)

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Reprints and permissions

About this article

Cite this article

Shen, Y., Dana, H., Abdelfattah, A.S. et al. A genetically encoded Ca²⁺ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578. BMC Biol 16, 9 (2018). https://doi.org/10.1186/s12915-018-0480-0

Download citation

Received: 29 November 2017
Accepted: 03 January 2018
Published: 16 January 2018
DOI: https://doi.org/10.1186/s12915-018-0480-0

A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578

Abstract

Background

Results

Conclusion

Similar content being viewed by others

Background

Results

Design and engineering of K-GECO1

In vitro characterization of K-GECO1

Crystal structure of K-GECO1

Performance of K-GECO1 in cultured cells

Performance of K-GECO1 in organotypic brain slices

In vivo Ca2+ imaging with K-GECO1

Discussion

Conclusion

Methods

Protein engineering

In vitro characterization

Protein crystallography

Cell culture and imaging

Organotypic hypothalamic rat brain slice imaging

Imaging of zebrafish spinal sensory neurons

Mouse V1 imaging

Statistical analysis

Change history

30 October 2019

References

Acknowledgements

Funding

Availability of data and materials

Author information

Authors and Affiliations

Contributions

Corresponding author

Ethics declarations

Ethics approval

Competing interests

Publisher’s Note

Additional files

Rights and permissions

About this article

Cite this article

Share this article

Keywords

Search

Navigation

A genetically encoded Ca²⁺ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578

In vivo Ca²⁺ imaging with K-GECO1