March 2013, 55:20,
Open Access This content is freely available online to anyone, anywhere at any time.
Date: 05 Mar 2013
Single layer centrifugation-selected boar spermatozoa are capable of fertilization in vitro
Good quality spermatozoa are important to achieve fertilization, viable embryos and offspring. Single Layer Centrifugation (SLC) through a colloid (Androcoll-P) selects good quality spermatozoa. However, it has not been established previously whether porcine spermatozoa selected by this method maintain their fertility.
The semen was prepared either by SLC or by standard centrifugation (control) and used for in vitro fertilization (IVF) at oocyte:spermatozoa ratios of 1:50; 1:100 and 1:300 (or 4 x 103, 8 x 103 and 24 x 103 spermatozoa/ml) to evaluate their subsequent ability to generate blastocysts. In addition, sperm motility was assessed by computer assisted sperm motility analysis.
Total and progressive motility were significantly higher in sperm samples prepared by SLC compared to uncentrifuged samples. Sperm binding ability, polyspermy, cleavage and blastocyst rates were affected by the oocyte:sperm ratio, but not by sperm treatment.
The use of SLC does not adversely affect the in vitro fertilizing and embryo-generating ability of the selected spermatozoa compared to their unselected counterparts, but further modifications in the IVF conditions would be needed to improve the monospermy in IVF systems. Since SLC did not appear to have a negative effect on sperm fertilizing ability, and may in fact select for spermatozoa with a greater potential for fertilization, an in vivo trial to determine the usefulness of this sperm preparation technique prior to artificial insemination is warranted.
Morrell J, Rodríguez-Martínez H: Biomimetic techniques for improving sperm quality in animal breeding: a review. The Open Andrology J 2009, 1:1–9.
Morrell J, Rodríguez-Martínez H: Practical applications of sperm selection techniques as a tool for improving reproductive efficiency. Vet Med International 2011., 2011: Article ID 894767
Thys M, Vandaele L, Morrell J, Mestach J, van Soom A, Hoogewijs M, Rodriguez-Martinez H: In vitro fertilizing capacity of frozen-thawed bull spermatozoa selected by single-layer (glycidoxypropyltrimethoxysilane) silane-coated silica colloidal centrifugation. Reprod Domest Anim 2009, 44:390–394.PubMedCrossRef
van Wienen M, Johannisson A, Wallgren M, Parlevliet J, Morrell J: Single layer centrifugation with Androcoll-P can be scaled-up to process larger volumes of boar semen. ISRN Vet Sci 2011., 2011: Article ID 548385
Morrell J, Wallgren M: Androcoll™-P-Large selects boar spermatozoa with good membrane integrity from the sperm-rich fraction of the ejaculate. Anim Reprod 2010, 7:16–20.
Caballero I, Vázquez JM, Gil MA, Calvete JJ, Roca J, Sanz L, Parrilla I, García EM, Rodríguez-Martínez H, Martínez E: Does seminal plasma PSP-I/PSP-II spermadhesin modulate the ability of boar spermatozoa to penetrate homologous oocytes in vitro? J Androl 2004, 25:1004–1012.PubMed
Simonsson A: Nutrient recommendations for pigs [in Swedish]. Uppsala, Sweden: Swedish University of Agricultural Sciences, SLU Info. Rapporter, Husdjur; 1994:75.
Petters R, Wells K: Culture of pig embryos. J Reprod Fertil Suppl 1993, 48:61–73.PubMed
Gordon I: Laboratory production of cattle embryos. Second edition. Dublin, Ireland: CABI Publishing; 2003. [Biotechnology in Agriculture Series No. 27]
Roca J, Rodríguez-Martínez H, Vázquez J, Bolarín A, Hernández M, Saravia F, Wallgren M, Martínez E: Strategies to improve the fertility of frozen-thawed boar semen for artificial insemination. Soc Reprod Fertil Suppl 2006, 62:261–275.PubMed
- Single layer centrifugation-selected boar spermatozoa are capable of fertilization in vitro
- Open Access
- Available under Open Access This content is freely available online to anyone, anywhere at any time.
Acta Veterinaria Scandinavica
- Online Date
- March 2013
- Online ISSN
- BioMed Central
- Additional Links
- Sperm selection
- Author Affiliations
- 1. Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, P.O. Box 7054, Uppsala, SE 750 07, Sweden
- 2. Centre for Reproductive Biology in Uppsala, Swedish University of Agricultural Sciences, P.O. Box 7054, Uppsala, SE 750 07, Sweden