Background
The only malaria vaccine to have been tested in Phase III clinical vaccine trials [1] induces protection which has been associated with high titres of antibodies against sporozoites. Efficacy against clinical malaria in infants of 5-17 months and 6-12 weeks was 55.8% and 31.3% respectively. A successful malaria vaccine will likely need to induce both cellular and humoral immunity in order to achieve a deployable level of efficacy in the target age groups. Prime-boost immunisation with viral vectors ChAd63 and MVA, both encoding ME-TRAP, has been shown to induce high T cell responses and modest antibody responses to the pre-erythrocytic malaria antigen TRAP. A Phase II study with this vaccine regime in malaria-naïve adults showed significant efficacy, which correlated with frequency of monofunctional CD8+ T cells secreting IFNγ. TRAP antibody titres were modest and did not correlate with protection [2]. In contrast to this, high titres of vaccine-induced anti-TRAP antibodies are measured in infants in malaria-endemic settings.
Materials and methods
Antibody titres were measured in 138 malaria-exposed children vaccinated with ChAd63 MVA ME-TRAP in three Phase I studies in The Gambia and Burkina Faso. Age groups at first immunisation were 2-6 years, 5-12 months and 10 weeks in The Gambia and 5-17 months in Burkina Faso. Avidity and isotype profiles were also analyzed.
Results
Antibody responses to TRAP were significantly higher in 10 week old and 5-12 month old infants in The Gambia and 5-17 month old infants in Burkina Faso compared to 2-6 year old children and adults in The Gambia and malaria-naïve UK adults. IgG isotype responses were predominantly IgG1 and IgG3 and we also detected IgA and IgM. TRAP-specific IgG avidity was significantly higher in Burkinabe infants aged 5-17 months and Gambian infants aged 5-12 months compared to Gambian adults and 2-6 year old children. TRAP-specific IgG1 avidity significantly correlated with age at vaccination in 5-17 month old Burkinabe infants and was significantly higher than in 10 week old Gambian infants. Functional activity of anti-TRAP antibodies will be analysed in vitro using a sporozoite invasion inhibition assay.
Conclusions
We demonstrate excellent humoral immunogenicity in key target populations vaccinated with a pre-erythrocytic malaria vaccination regime, exceeding that seen in UK malaria-naïve adults.
References
Vansadia P: A Phase 3 Trial of RTS,S/AS01 Malaria Vaccine in African Infants. N Engl J Med. 2012, 367: 2284-2295.
Ewer K, O’Hara G: Protective CD8+ T-cell immunity to human malaria induced by chimpanzee adenovirus-MVA immunisation. Nat Commun. 2013, 4:
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Bowyer, G., Afolabi, M.O., Bliss, C.M. et al. Humoral immunogenicity of ChAd63_MVA ME-TRAP vaccination in African infants and children. Malar J 13 (Suppl 1), P16 (2014). https://doi.org/10.1186/1475-2875-13-S1-P16
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DOI: https://doi.org/10.1186/1475-2875-13-S1-P16