Abstract.
The internal structure of DNA-polycation complexes is investigated by synchrotron small-angle X-ray scattering (SAXS). Hexagonal packing of DNA is observed for DNA complexed with poly-L-lysine (PL), poly-L-arginine (PA), spermine (Sp), and linear and branched polyethyleneimine (lPEI and bPEI, respectively). Variations in the internal spacings and degree of long-range ordering are dependent on both polycation type and concentration of added salt. With increasing concentration of monovalent salt, a discontinuous phase transition is observed from compact to loose bundles and finally to an isotropic network phase. This salt-induced melting transition was found to be universal for all polyplexes studied and is in quantitative agreement with a simple free energy model based solely on electrostatic and entropic contributions. Using the osmotic stress method, bulk modulus (K) is measured for PL-DNA and PA-DNA polyplexes at various salt concentrations. With increasing osmotic force, we show that the salt-induced melting transition is shifted and compression in the loose bundle regime is in qualitative agreement with our model.
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DeRouchey, J., Netz, R. & Rädler, J. Structural investigations of DNA-polycation complexes. Eur. Phys. J. E 16, 17–28 (2005). https://doi.org/10.1140/epje/e2005-00003-4
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DOI: https://doi.org/10.1140/epje/e2005-00003-4