Abstract
The peculiarities of the in vitro regeneration of the rare species Fritillaria meleagris L. from floral explants have been studied for the first time. Upon the establishment of in vitro culture and during the multiplication stage, the most effective is the B5 nutrient medium supplemented with 0.44 µM BAP, 3.22 µM NAA, and 2.28 µM IAA. During the multiplication stage, the regeneration rate reaches 80% and the number of bulblets per explant is 3.9 ± 0.3. It is established that the morphogenesis of de novo formed structures depends on the mineral composition of the medium: the use of mineral-based B5 leads to adventitious shoot formation (gemmogenesis), while the use of BDS causes morphogenic callus formation and the gemmorhizogenesis on its surface. The stimulating effect of low temperatures (+7°C) on bulblet development and adaptation to ex vitro conditions is noted.
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Batygina, T.B., Kruglova, N.N., Gorbunova, V.Yu., et al., Ot mikrospory — k sortu (From Microspore to the Cultivar), Moscow: Nauka, 2010.
De Bruyn, M.H., Ferreira, D.I., Slabbert, M.M., and Pretorius, J., In vitro propagation of Amaryllis belladonna, Plant Cell, Tissue Organ Cult., 1992, no. 31, pp. 179–184.
Dunstan, D.J. and Short, K.C., Improved growth of tissue cultures of the onion Allium cepa, Physiol. Plant, 1977, vol. 41, no. 1, pp. 70–72.
Engelmann, F., Use of biotechnologies for the conservation of plant biodiversity, In Vitro Cell. Dev. Biol.: Plant, 2011, vol. 47, pp. 5–16.
Erst, A.A., Erst, A.S., Shaulo, D.N., and Kul’khanova, D.S., Preservation and reproduction in vitro of rare species of genus Fritillaria (Liliaceae), Rastit. Mir Aziat. Ross., 2014, no. 1(13), pp. 64–70.
Gamborg, O.L. and Eveleigh, D.E., Culture methods and detection of glucanases in cultures of wheat and barley, Can. J. Biochem., 1968, vol. 46, no. 5, pp. 417–421.
Gao, S.L., Zhu, D.N., Cai, Z.H., Jiang, Y., and Xu, R., Organ culture of a precious Chinese medicinal plant — Fritillaria unibracteata, Plant Cell, Tissue Organ Cult., 1999, no. 59, pp. 197–201.
Jevremovič, S., Petrič, M., Živkovič, S., Trifunovič, M., and Subotič, A., Superoxide dismutase activity and isoenzyme profiles in bulbs of snake’s head fritillary in response to cold treatment, Arch. Biol. Sci., 2010, no. 62, no. 3, pp. 553–558.
Jiménez, V.M., Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis, Plant Growth Regul., 2005, no. 47, pp. 91–110.
Joshi, S.K., Dhar, U., and Andola, H.C., In vitro bulblet regeneration and evaluation of Fritillaria roylei Hook. a high value medicinal herb of the Himalaya, Acta Hortic., 2007, no. 756, pp. 75–84.
Kalinin, F.L., Sarnatskaya, V.V., and Polishchuk, V.E., Metody kul’tury tkanei v fiziologii i biokhimii rastenii (The Methods of Tissue Culture in Physiology and Biochemistry of the Plants), Kyiv: Naukova Dumka, 1980.
Kim, M.S., Jeon, J.H., Youm, J.W., Kim, J.H., Lee, B.C., Kang, W.J., Kim, H.S., and Joung, H., Efficient plantlet regeneration via callus formation from leaf segment of Lilium oriental hybrid “Casa Blanca”, J. Plant. Biotech., 2005, no. 7, pp. 129–134.
Krasnaya kniga Rossiiskoi Federatsii: rasteniya i griby (The Red Data Book of Russian Federation: Plants and Fungi), Moscow: KMK, 2008.
Laslo, V., Zăpârâan, M., and Agud, E., In vitro conservation of certain endangered and rare species of Romanian spontaneous flora, An. Univ. Oradea, Fasc. Prot. Med., 2011, vol. 16, pp. 252–261.
Mohammadi-Dehcheshmeh, M., Khalighi, A., Naderi, R., Ebrahimie, E., and Sardari, M., Indirect somatic embryogenesis from petal explant of endangered wild population of Fritillaria imperialis, Pak. J. Biol. Sci., 2007, vol. 10, no. 11, pp. 1875–1879.
Mohammadi-Dehcheshmeh, M., Khalighi, A., Naderi, R., Sardari, M., and Ebrahimie, E., Petal: a reliable explant for direct bulblet regeneration of endangered wild populations of Fritillaria imperialis L., Acta Physiol., 2008, no. 30, pp. 395–399
Nikolaeva, M.G., Lyanguzova, I.V., and Pozdova, L.M., Biologiya semyan (Biology of Seeds), St. Petersburg: Nauchno-Issled. Inst. Khim., S.-Peterb. Gos. Univ., 1999.
Nikolič, M., Mišič, D., Maksimovič, V., Jevremovič, S., Trifunovič, M., and Subotič, A., Effect of low temperature on rooting rate and carbohydrate content of Fritillaria meleagris bulbs formed in culture in vitro, Arch. Biol. Sci., 2008, vol. 60, no. 1, pp. 5–6.
Otani, M. and Shimada, T., Micripropagation of Fritillaria camtschatcensis (L.) KerGawl. “Kuroyuri,” Bull. Res. Inst. Agric. Sci., Ishikawa Agric. Coll., 1997, vol. 5, pp. 39–44.
Paek, K.Y. and Murthy, H.N., High frequency of bulblet regeneration from bulb scale sections of Fritillaria thunbergii, Plant Cell, Tissue Organ Cult., 2002, no. 68, pp. 247–252.
Pausheva, Z.P., Prkatikum po tsitologii rastenii (Practical Manual on the Plant Cytology), Moscow: Agropromizdat, 1988.
Petrič, M., Subotič, A., Jevremovič, S., and Trifunovič, M., Somatic embryogenesis and bulblet regeneration in snakehead fritillary (Fritillaria meleagris L.), Afr. J. Biotech., 2011, vol. 72, no. 10, pp. 16181–16188.
Ramage, C.M. and Williams, R.R., Mineral nutrition and plant morphogenesis, In Vitro Cell. Dev. Biol.: Plant, 2002, no. 38, pp. 116–124.
Slabbert, M.M., De Bruyn, M.H., Ferreira, D.I., and Pretorius, J., Regeneration of bulblets from twin scales of Crinum macowanii in vitro, Plant Cell, Tissue Organ Cult., 1993, no. 33, pp. 133–141.
Subotič, A., Trifunovič, M., Jevremovič, S., and Petrič, M., Morpho-histological study of direct somatic embryogenesis in endangered species Frittilaria meleagris, Biol. Plant, 2010, vol. 54, no. 3, pp. 592–596.
Vechernina, N.A., Metody biotekhnologii v selektsii, razmnozhenii i sokhranenii genofonda rastenii: monografiya (The Methods of Biotechnology in Selection, Reproduction, and Preservation of the Gene Pool of the Plants: Monograph), Barnaul: Altaisk. Gos. Univ., 2004.
Vetchinkina, E.M., Biological features of cultivation in vitro of seeds and embryos of rare plant species, Extended Abstract of Cand. Sci. (Biol.) Dissertation, Moscow, 2010.
Vetchinkina, E.M., Shirnina, I.V., Shirnin, S.Yu., and Molkanova, O.I., Preservation of rare plant species in genetic collections in vitro, Vestn. Balt. Fed. Univ. im. I. Kanta, 2012, no. 7, pp. 109–118.
Xu, L.F., Ma, F.W., and Liang, D., Plant regeneration from in vitro cultured leaves of Lanzhou lily (Lilium davidii var. unicolor), Sci. Hortic., 2009, no. 119, pp. 458–461.
Yae, B.W., Han, B.H., and Goo, D.H., Dormancy breaking and in vitro growth of in vitro bulblets in Lilium oriental hybrid “Casablanca,” J. Kor. Soc. Hortic. Sci., 2001, no. 42, pp. 99–102.
Yan, M.-M., Xu, C., Kim, C.-H., Um, Y.-C., Bah, A.A., and Guo, D.-P., Effects of explant type, culture media and growth regulators on callus induction and plant regeneration of Chinese jiaotou (Allium chinense), Sci. Hortic., 2009, vol. 123, no. 1, pp. 124–128.
Ziv, M. and Lilien-Kipnis, H., Bud regeneration from inflorescence explants for rapid propagation of geophytes in vitro, Plant Cell Rep., 2000, no. 19, pp. 845–850.
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Original Russian Text © D.S. Muraseva, T.I. Novikova, A.A. Erst, 2015, published in Sibirskii Ekologicheskii Zhurnal, 2015, No. 6, pp. 909–919.
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Muraseva, D.S., Novikova, T.I. & Erst, A.A. In vitro propagation and conservation of rare species Fritillaria meleagris L. from floral explants. Contemp. Probl. Ecol. 8, 754–763 (2015). https://doi.org/10.1134/S1995425515060128
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DOI: https://doi.org/10.1134/S1995425515060128