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Simultaneous detection and quantification of zeatin and kinetin in coconut water using ultra performance liquid chromatography coupled with a simple step solid phase extraction

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Abstract

Trans-zeatin and kinetin are two cytokinin family members that exhibit an anti-aging effect on human fibroblast cells. These cytokinins can be found in coconut water and in the xylem sap of most plants. This study describes the first attempt of a simultaneous separation of zeatin and kinetin using a simple C18 solid phase extraction (SPE) method coupled with ultra performance liquid chromatography (UPLC). This method provided high recoveries of 95.2 and 98.6% for trans-zeatin and kinetin, respectively. Malayan Green Dwarf, a local coconut variety, was found to contain 14 μg/L trans-zeatin and 4 μg/L kinetin. This method will be useful for comprehensive research on different coconut varieties and determining the contents of cytokinins in coconut water.

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Authors and Affiliations

  1. Innovation and Commercialisation Division, Ministry of Science, Technology and Innovation (MOSTI) Level 3, Block C4, Complex C, Federal Government Administrative Centre, 62662, Putrajaya, Malaysia

    C. M. Y. Mooi

  2. Biotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), PO Box 12301, Pejabat Besar Pos, 50774, Kuala Lumpur, Malaysia

    S. P. Koh & K. Long

Authors
  1. C. M. Y. Mooi
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  2. S. P. Koh
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  3. K. Long
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Correspondence to K. Long.

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Mooi, C.M.Y., Koh, S.P. & Long, K. Simultaneous detection and quantification of zeatin and kinetin in coconut water using ultra performance liquid chromatography coupled with a simple step solid phase extraction. J Anal Chem 70, 819–824 (2015). https://doi.org/10.1134/S1061934815070114

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  • DOI: https://doi.org/10.1134/S1061934815070114

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