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Capillary electrophoresis coupled with microdialysis for continuous monitoring of free metoprolol in rabbit blood

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Abstract

A microdialysis method followed by a capillary electrophoresis (CE) procedure has been performed for the determination of metoprolol in rabbit blood. A microdialysis probe was inserted into the ear vein for blood sampling. The electrophoresis conditions were: 47 (40) cm × 50 μm silica capillary, 50 mM acetate buffer solution (pH 4.0), 10 s hydrodynamic load, detection wavelength 214 nm, run voltage 18 kV, temperature 25°C. The calibration was linear within the range of 1.0–100 μg/mL (r > 0.999). The limit of detection (LOD) was 0.5 μg/mL. Intra-day and inter-day repeatabilities with relative standard deviations (RSDs) ≤ 5.1% were obtained. The method was then applied to pharmacokinetics profiling of metoprolol in the blood following oral administration of metoprolol to rabbits.

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Authors and Affiliations

  1. Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang, 212013, P.R. China

    Li Wang

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  1. Li Wang
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Correspondence to Li Wang.

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Wang, L. Capillary electrophoresis coupled with microdialysis for continuous monitoring of free metoprolol in rabbit blood. J Anal Chem 70, 203–206 (2015). https://doi.org/10.1134/S1061934815020100

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  • DOI: https://doi.org/10.1134/S1061934815020100

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