Abstract
An electrophoretic assay of lactate dehydrogenase (EC 1.1.1.27) isozymes in the tissue homogenates of cardiac and skeletal muscles, kidney, lungs, spleen, and liver of the raccoon dog Nyctereutes procyonoides from two different geographic zones, viz., northwestern Russia and Poland, as well as the Arctic blue fox Alopex lagopus L. and the red fox Vulpes vulpes L. was performed during the preparatory period to the winter season. Raccoon dogs, which hibernate under natural conditions, differ from other canids (the red fox and Arctic blue fox) to which they are close taxonomically by their body weight and by the higher proportion of aerobic H subunits of lactate dehydrogenase in all organs except for the heart. A higher content of “fast” anode fractions, lactate dehydrogenase-1 and lactate dehydrogenase-2, in the heart, kidney, lungs, liver, and spleen was detected in the raccoon dogs from the northern region compared to those from the southern geographic zone. The shift in the reaction catalyzed by lactate dehydrogenase towards the production of pyruvate indicates that this metabolite is necessary for the synthesis of fatty acids during lipogenesis in the autumn.
Similar content being viewed by others
Abbreviations
- LDH:
-
lactate dehydrogenase
References
P. W. Hochachka and G. N. Somero, Biochemical Adaptation: Mechanism and Process in Physiological Evolution (Oxford Univ. Press, New York, 1984; Mir, Moscow, 1988).
N. I. Kalabukhov, Hibernation in Mammals (Nauka, Moscow, 1985) [in Russian].
H. V. Carey, M. T. Andrews, and S. L. Martin, Physiol. Rev. 83, 1153 (2003).
V. A. Berestov and L. K. Kozhevnikov, Blood Enzymes of Fur-bearing Mammals (Nauka, Leningrad, 1981) [in Russian].
A. A. Nasimovich, in Arctic Fox, Red Fox, and Raccoon Dog (Nauka, Moscow, 1985), pp. 116–146 [in Russian].
P. Lapa, S. Lapinski, V. Slivyak, et al., Vestn. VOGiS, 13 (3), 647 (2009).
I. L. Tumanov, Specific Biological Features of Predatory Mammals in Russia (Nauka, St. Petersburg, 2003) [in Russian].
J. Asikainen, A. M. Mustonen, H. Hyvärinen, and P. Nieminen, Zool. Sci. 21 385 (2004).
P. I. Danilov, New Mammal Species in the North of European Russia (Karel. Sci. Center. Russ. Acad. Sci., Petrozavodsk, 2009) [in Russian].
A. V. Parshintsev, Nauch. Tr. Gos. Prir. Zap. Prisurskii 30 (2), 13 (2015).
E. V. Karaduleva, I. M. Santalova, and N. M. Zakharova, Biophysics (Moscow) 59 (5), 752 (2014).
P. I. Danilov, O. S. Rusakov, and I. P. Tumanov, Predatory Mammals in the Northwestern Soviet Union (Nauka, Leningrad, 1979) [in Russian].
C. C. Rider and C. B. Taylor, Isoenzymes (Chapman and Hall, London, 1980; Mir, Moscow, 1983).
L. K. Kozhevnikova, N. N. Tyutyunnik, A. R. Unzhakov, et al., Zh. Evol. Biokhim. Fiziol. 36 (1), 24 (2000).
L. K. Kozhevnikova, N. N. Tyutyunnik, A. R. Unzhakov, et al., Ross. Fiziol. Zh. im._I. M. Sechenova 90 (2), 187 (2004).
A. R. Unzhakov and N. N. Tyutyunnik, Zh. Evol. Biokhim. Fiziol. (in press).
J. Everse and O. N. Kaplan, Adv. Enzymol. 37, 61 (1973).
P. Marmillot, T. Keith, D. K. Srivastava, and H. R. Knull, Arch. Biochem. Biophys. 315 (2) 467 (1994).
R. P. Singh, K. V. H. Sastry, N. K. Pandey, et al., Theriogenology 75 (3), 555 (2011).
E. Gaal, G. Meguesi, and L, Vereczkei, Electrophoresis in Separation of Biological Macromolecules (Mir, Moscow, 1982) [in Rssian].
R. Wieme, Studies on Agar-gel Electrophoresis (Brussels, 1959).
T. Sayd, T. Mera, and M. Veronique, Comp. Biochem. Physiol. 120B (1), 153 (1998).
V. I. Afromeev and V. N. Tkachenko, Biophysics (Moscow) 44 (5), 903 (1999).
G. T. Favero, S. Stavriaanes, and G. A. Klug, Exp. Physiol. 84, 989 (1999).
P. Prestrud, Arctic 44, 132 (1991).
Yu. S. Zabolotskikh, Doctoral Dissertation in Biology (Moscow, 2000).
H. Korhonen, Comp. Biochem. Physiol. 91A (2), 263 (1988).
H. Korhonen and M. Harri, Comp. Biochem. Physiol. 77A, 213 (1984).
M. Mustonen, J. Asikainen, K. Kauhala, et al., Chronobiol. Int. 24 1095 (2007).
H. Korhonen, M. Harri, and E. Hohtola, Comp. Biochem. Physiol. 82A (4), 959 (1985).
Author information
Authors and Affiliations
Corresponding authors
Additional information
Original Russian Text © A.R. Unzhakov, N.N. Tyutyunnik, 2016, published in Biofizika, 2016, Vol. 61, No. 4, pp. 758–765.
Rights and permissions
About this article
Cite this article
Unzhakov, A.R., Tyutyunnik, N.N. The isozyme spectra of lactate dehydrogenase in the tissues of the raccoon dog Nyctereutes procyonoides in the autumn. BIOPHYSICS 61, 640–646 (2016). https://doi.org/10.1134/S0006350916040254
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006350916040254