Abstract
The activity of a key enzyme of the cytochrome component of the respiratory chain (cytochrome oxidase), the quantitative redistribution of mitochondrial cytochromes b, c 1, c, and aa 3, as well as the activities of the key enzymes of cytochrome heme metabolism (δ-aminolevulinate synthase and heme oxygenase) under conditions of acetaminophen-induced liver injury were studied on the background of dietary protein deprivation. Under conditions of acetaminophen-induced hepatitis that developed on the background of alimentary protein deprivation, an inhibition of cytochrome oxidase activity and a decrease in the contents of mitochondrial cytochromes on the background of an increase in the δ-aminolevulinate synthase and heme oxygenase activity were observed. In animals with a toxic liver injury that were kept under conditions of dietary protein deprivation, the contents of mitochondrial cytochromes b, c 1, c, and aa 3 progressively decreased, which was accompanied by an increase in heme oxygenase activity, whereas δ-aminolevulinate synthase activity remained at the control level. It was concluded that dietary protein deprivation is a critical factor for the development of disturbances in the structural-functional integrity of the cytochrome component of the respiratory chain. The identified changes can be considered as a possible mechanism that underlies the disturbance in the function of the energy biotransformation system under conditions of dietary protein deprivation.
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Original Russian Text © O.N. Voloshchuk, G.P. Kopylchuk, 2015, published in Biofizika, 2015, Vol. 60, No. 3, pp. 519–524.
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Voloshchuk, O.N., Kopylchuk, G.P. The peculiarities of the structural and functional state of the cytochrome component of the liver mitochondrial respiratory chain under conditions of acetaminophen-induced hepatitis on the background of alimentary protein deprivation. BIOPHYSICS 60, 420–424 (2015). https://doi.org/10.1134/S0006350915030215
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DOI: https://doi.org/10.1134/S0006350915030215