Optics and Spectroscopy

, Volume 114, Issue 3, pp 471–477

Compact low-cost detector for in vivo assessment of microphytobenthos using laser induced fluorescence

Authors

    • INOV-INESC Inovação
    • ICEMS, Instituto Superior TécnicoTechnical University of Lisbon
  • S. Vieira
    • Centro de OceanografiaFaculdade de Ciências da Universidade de Lisboa
    • BioFIGFaculdade de Ciências da Universidade de Lisboa
    • Departamento de Biologia VegetalFaculdade de Ciências da Universidade de Lisboa
  • J. Marques da Silva
    • BioFIGFaculdade de Ciências da Universidade de Lisboa
    • Departamento de Biologia VegetalFaculdade de Ciências da Universidade de Lisboa
  • A. Lavrov
    • INOV-INESC Inovação
    • ICEMS, Instituto Superior TécnicoTechnical University of Lisbon
  • E. Leite
    • INOV-INESC Inovação
    • ICEMS, Instituto Superior TécnicoTechnical University of Lisbon
  • P. Cartaxana
    • Centro de OceanografiaFaculdade de Ciências da Universidade de Lisboa
Lasers and Their Applications

DOI: 10.1134/S0030400X13030259

Cite this article as:
Utkin, A.B., Vieira, S., Marques da Silva, J. et al. Opt. Spectrosc. (2013) 114: 471. doi:10.1134/S0030400X13030259

Abstract

The development of a compact low-cost detector for non-destructive assessment of microphytobenthos using laser induced fluorescence was described. The detector was built from a specially modified commercial miniature fiber optic spectrometer (Ocean Optics USB4000). Its usefulness is experimentally verified by the study of diatom-dominated biofilms inhabiting the upper layers of intertidal sediments of the Tagus Estuary, Portugal. It is demonstrated that, operating with a laser emitter producing 30 mJ pulses at the wavelength of 532 nm, the detector is capable to record fluorescence signals with sufficient intensity for the quantitative biomass characterization of the motile epipelic microphytobenthic communities and to monitor their migratory activity. This paves the way for building an entire emitter-detector LIF system for microphytobenthos monitoring, which will enable microalgae communities occupying hardly accessible intertidal flats to be monitored in vivo at affordable cost.

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Copyright information

© Pleiades Publishing, Ltd. 2013