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Purification and characteristics of xyloglucanase and five other cellulolytic enzymes from Trichoderma reesei QM9414

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Abstract

By combining anion-exchange chromatography with gel filtration, an effective method for purification of wild-type xyloglucanase and five other cellulolytic enzymes from strain QM9414 of Trichoderma reesei was established. Characterization by enzyme activity assay, SDS-PAGE, and mass spectrometry identified the purified proteins as cellobiohydrolases I and II, endoglucanases I and II, a xyloglucanase, and β-xylosidase, of which the xyloglucanase was purified for the first time from the mutant strain QM9414. This method holds great promise to study the mechanism of cellulolytic enzymes, to investigate the synergistic action between cellulase and other cellulolytic enzymes, and to better exploit enzyme preparations for degradation of lignocellulose.

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References

  1. Jarvis, M. (2003) Nature, 426, 611–612.

    Article  PubMed  CAS  Google Scholar 

  2. Huang, R. L., Su, R. X., Qi, W., and He, Z. M. (2011) Bioenerg. Res., 4, 225–245.

    Article  Google Scholar 

  3. Willis, J. D., Oppert, C., and Jurat-Fuentes, J. L. (2010) Insect. Sci., 17, 184–198.

    Article  CAS  Google Scholar 

  4. Lantz, S. E., Goedegebuur, F., Hommes, R., Kaper, T., Kelemen, B. R., Mitchinson, C., Wallace, L., Stahlberg, J., and Larenas, E. A. (2010) Biotechnol. Biofuels, 3, 20.

    Article  PubMed  Google Scholar 

  5. Zhou, L., Wu, X., Lan, L., and Liu, J. (2010) Biotechnol. Lett., 32, 67–72.

    Article  PubMed  Google Scholar 

  6. Montenecourt, B. S., and Eveleigh, D. E. (1977) Appl. Environ. Microbiol., 34, 777–782.

    PubMed  CAS  Google Scholar 

  7. Zhang, J., Zhong, Y., Zhao, X., and Wang, T. (2010) Bioresour. Technol., 101, 9815–9818.

    Article  PubMed  CAS  Google Scholar 

  8. Gao, D., Chundawat, S. P. S., Krishnan, C., Balan, V., and Dale, B. E. (2010) Bioresour. Technol., 101, 2770–2781.

    Article  PubMed  CAS  Google Scholar 

  9. Wang, F. C., Li, F., Chen, G. J., and Liu, W. F. (2009) Microbiol. Res., 164, 650–657.

    Article  PubMed  CAS  Google Scholar 

  10. Tomaz, C. T., and Queiroz, J. A. (2004) Biotechnol. Lett., 26, 223–227.

    Article  PubMed  CAS  Google Scholar 

  11. Homma, T., Fujii, M., and Titus, U. (1992) J. Chem. Eng. Jpn., 25, 288–293.

    Article  CAS  Google Scholar 

  12. Schmid, G., and Wandrey, C. (1987) Biotechnol. Bioeng., 30, 571–585.

    Article  PubMed  CAS  Google Scholar 

  13. Bhikhabhai, R., Johansson, G., and Pettersson, G. (1984) J. Appl. Biochem., 6, 336–345.

    PubMed  CAS  Google Scholar 

  14. Duenas, M. J., and Estrada, P. (1999) Biocatal. Biotransform., 17, 139–161.

    Article  CAS  Google Scholar 

  15. Deshpande, P., Nair, S., and Khedkar, S. (2009) Appl. Biochem. Biotech., 158, 552–560.

    Article  CAS  Google Scholar 

  16. Bradford, M. M. (1976) Anal. Biochem., 72, 248–254.

    Article  PubMed  CAS  Google Scholar 

  17. Shevchenko, A., Wilm, M., Vorm, O., Jensen, O. N., Podtelejnikov, A. V., Neubauer, G., Mortensen, P., and Mann, M. (1996) Biochem. Soc. Trans., 24, 893–896.

    PubMed  CAS  Google Scholar 

  18. Hu, R. F., Lin, L., Liu, T. J., Ouyang, P., He, B. H., and Liu, S. J. (2008) J. Biobased Mater. Bio., 2, 156–161.

    Article  Google Scholar 

  19. Subramaniyan, S., Prema, P., and Ramakrishna, S. V. (1997) J. Basic Microb., 37, 431–437.

    Article  CAS  Google Scholar 

  20. Hahn-Hagerdal, B., Galbe, M., Gorwa-Grauslund, M. F., Liden, G., and Zacchi, G. (2006) Trends Biotechnol., 24, 549–556.

    Article  PubMed  CAS  Google Scholar 

  21. Shoemaker, S., Watt, K., Tsitovsky, G., and Cox, R. (1983) Nat. Biotechnol., 1, 687–690.

    Article  CAS  Google Scholar 

  22. Gao, L., Wang, F., Gao, F., Wang, L., Zhao, J., and Qu, Y. (2011) Bioresour. Technol., 102, 8339–8342.

    Article  PubMed  CAS  Google Scholar 

  23. Foreman, P. K., Brown, D., Dankmeyer, L., Dean, R., Diener, S., Dunn-Coleman, N. S., Goedegebuur, F., Houfek, T. D., England, G. J., Kelley, A. S., Meerman, H. J., Mitchell, T., Mitchinson, C., Olivares, H. A., Teunissen, P. J., Yao, J., and Ward, M. (2003) J. Biol. Chem., 278, 31988–31997.

    Article  PubMed  Google Scholar 

  24. Zhang, J., Tuomainen, P., Siika-Aho, M., and Viikari, L. (2011) Bioresour. Technol., 102, 9090–9095.

    Article  PubMed  CAS  Google Scholar 

  25. Alvira, P., Negro, M. J., and Ballesteros, M. (2011) Bioresour. Technol., 102, 4552–4558.

    Article  PubMed  CAS  Google Scholar 

  26. Beukes, N., and Pletschke, B. I. (2010) Bioresour. Technol., 101, 4472–4478.

    Article  PubMed  CAS  Google Scholar 

  27. Qing, Q., Yang, B., and Wyman, C. E. (2010) Bioresour. Technol., 101, 9624–9630.

    Article  PubMed  CAS  Google Scholar 

  28. Burgess, A. W., Lloyd, C. J., and Nice, E. C. (1983) EMBO J., 2, 2065–2069.

    PubMed  CAS  Google Scholar 

  29. Beldman, G., Searle-Van Leeuwen, M. F., Rombouts, F. M., and Voragen, F. G. (1985) Eur. J. Biochem., 146, 301–308.

    Article  PubMed  CAS  Google Scholar 

  30. Hakansson, U., Fagerstam, L. G., Pettersson, L. G., and Andersson, L. (1979) Biochem. J., 179, 141–149.

    PubMed  CAS  Google Scholar 

  31. Gusakov, A. V., Grishutin, S. G., Markov, A. V., Ustinov, B. B., Semenova, M. V., and Sinitsyn, A. P. (2004) BBA-Gen Subjects, 1674, 268–281.

    Article  Google Scholar 

  32. Bukhtojarov, F. E., Ustinov, B. B., Salanovich, T. N., Antonov, A. I., Gusakov, A. V., Okunev, O. N., and Sinitsyn, A. P. (2004) Biochemistry (Moscow), 69, 542–551.

    Article  CAS  Google Scholar 

  33. Nikupaavola, M. L., Lappalainen, A., Enari, T. M., and Nummi, M. (1985) Biochem. J., 231, 75–81.

    CAS  Google Scholar 

  34. Golubev, A. M., Brandao Neto, J. R., Eneyskaya, E. V., Kulminskaya, A. V., Kerzhner, M. A., Neustroev, K. N., and Polikarpov, I. (2000) Acta Crystallogr. D. Biol. Crystallogr., 56, 1058–1060.

    Article  PubMed  CAS  Google Scholar 

  35. Poutanen, K., and Puls, J. (1988) Appl. Microbiol. Biot., 28, 425–432.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Qingdao Institute of Bioenergy and Bioprocess Technology and Key Laboratory of Bioenergy, Chinese Academy of Sciences, 189 Songling Road, Qingdao, 266101, China

    Huan Qi, Fali Bai & Aihua Liu

  2. University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing, 100049, China

    Huan Qi & Aihua Liu

Authors
  1. Huan Qi
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  2. Fali Bai
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  3. Aihua Liu
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Correspondence to Aihua Liu.

Additional information

Published in Russian in Biokhimiya, 2013, Vol. 78, No. 4, pp. 548–555.

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Qi, H., Bai, F. & Liu, A. Purification and characteristics of xyloglucanase and five other cellulolytic enzymes from Trichoderma reesei QM9414. Biochemistry Moscow 78, 424–430 (2013). https://doi.org/10.1134/S0006297913040123

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  • DOI: https://doi.org/10.1134/S0006297913040123

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