Abstract
A high-performance liquid chromatographic method for the simultaneous determination of oxypaeoniflorin, paeoniflorin, ferric acid, naringin, and neohesperidin in a traditional Chinese medicinal preparation, refined xue-fu-zhu-yu-tang, was developed. Separations were carried out with a ZORBAX SB-C18 column along with an Agilent C18 precolumn by linear gradient elution using 1% acetic acid-methanol (v/v: 0 min, 80/20; 15 min, 70/30; 35 min, 60/40; 55 min, 60/40) as the mobile phase at a flow rate of 0.5 mL/min. The analytes were detected with a diode-array detector at their maximum UV wavelengths. The correlation coefficients of the calibration curve for the analytes exceeded 0.9999. The recoveries were in the range 95.95–104.42 % with RSD less than 1.85%, and the precision of this method was better than 1.80%. The effects of several factors on the chromatographic behavior and sample extraction of the analytes were compared. In addition, the contents of these bioactive constituents in six different samples of this preparation were analyzed by using the optimized high-performance liquid chromatographic method.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Xue, T.H. and Roy, R., Science, 2003, vol. 300, p. 740.
Sun, Y.Q., Zhou, L.L., and Chen, X.Y., China Pharm., 2003, vol. 4, p. 22.
Li, X.Y., J. Chin. Clin. Med., 2003, vol. 4, p. 121.
Hao, H.Y., Guo, J.X., Shun, Q.S., Liang, J., Yu, Y.X., and Che, Z.T., Acta Pharm. Sin., 2000, vol. 35, p. 289.
Wang, W.X., Jiang, X.G., Gu, M., and Gu, Z.L., China J. Chin. Mater. Med., 2001, vol. 26, p. 543.
Wang, W., Chen, K.Y., Shi, D.Z., Zhong, P., and Zhu, Y.B., J. Integr. Tradit. West. Med., 1996, vol. 16, p. 670.
Wang, W., Ma, X.C., Wang, X.F., Shi, D.Z., and Chen, K.Y., J. Tradit. Chin. Med., 2000, vol. 41, p. 471.
Cai, Y.P., Chao, L., and Fan, C.S., J. JiangXi College Tradit. Chin. Med., 1999, vol. 11, p. 18.
Bok, S.H., Shin, Y.W., Bae, K.H., Jeong, T.S., Kwon Y.K., Park Y.B., and Choi M.S., Nutr. Res., 2000, vol. 20, p. 1007.
Liu, C., Wang, J., and Yang, J., J. Chin. Med. Mater., 2000, vol. 23, p. 557.
Zhang, M.F., J. Shaixi Provincial College of Traditional Chinese Medicine and Material Medica, 1990, vol. 21, p. 41.
Liu, C.Y., Tang, L.H., Xu, X.Y., Gu, B.L., and Zhang, Q.Y., Chin. Tradit. Patent Med., 1998, vol. 20, p. 9.
Li, Z.R., Wang, X., and Zhou, H., West China J. Pharm. Sci., 1995, vol. 10, p. 141.
Ji, F., Lu, X.F., Ruan, Q., He, Q.H., and He, X.X., Chin. JMAP, 2003, vol. 20, p. 149.
Li, X.L., Li, L., Xiao, H.B., and Liang, X.M., Chin. J. Chromatogr., 2002, vol. 20, p. 585.
Wang, X.F., Chen, K.Y., Wang, W., Shi, D.Z., Zhou, P.Y., and Chen, K.Y., J. Integr. Tradit. West. Med., 1998, vol. 18, p. 399.
Author information
Authors and Affiliations
Additional information
The text was submitted by the authors in English.
Rights and permissions
About this article
Cite this article
Luan, Lj., Mi, Sy., Hu, L. et al. Simultaneous determination of five bioactive constituents in the traditional Chinese medicinal preparation refined xue-fu-zhu-yu-tang by high-performance liquid chromatography with diode-array UV detection. J Anal Chem 61, 139–146 (2006). https://doi.org/10.1134/S1061934806020092
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1134/S1061934806020092