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Structure of hexasaccharide 1-phosphate polymer from Arthrobacter uratoxydans VKM Ac-1979T cell wall

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Abstract

A hexasaccharide 1-phosphate polymer of original structure and two teichoic acids (TA) belonging to different structural types were found in Arthrobacter uratoxydans VKM Ac-1979T cell wall. The poly(hexasaccharide 1-phosphate) combines features of teichuronic acids and glycosyl 1-phosphate polymers, and its structure has never been reported earlier. Its composition includes residues of α- and β-D-glucuronic acid as well as α-D-galacto-, β-D-gluco-, α-D-mannopyranose, and 6-O-acetylated 2-acetamido-2-deoxy-α-D-glucopyranose. The phosphodiester bond in the polymer joins the glycoside hydroxyl of α-D-glucuronic acid and O6 of α-D-galactopyranose. TA 1 is β-D-glucosylated 1,3-poly(glycerol phosphate), and TA 2 is 3,6-linked poly[α-D-glucosyl-(1→2)-glycerol phosphate]. The phosphate-containing polymers were studied by chemical methods and on the basis of one-dimensional 1H-, 13C-, and 31P-NMR spectra, homonuclear two-dimensional 1H/1H COSY, TOCSY, ROESY, and heteronuclear 1H/13C HSQC, HSQC-TOCSY, HMBC, and 1H/31P HMBC experiments. The set and structure of the polymers revealed as well as the cell wall sugars (galactose, glucose, mannose, glucosamine) and glycerol can be used in microbiological practice for taxonomic purposes.

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Authors and Affiliations

  1. Biological Faculty, Lomonosov Moscow State University, 119991, Moscow, Russia

    N. V. Potekhina

  2. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 117913, Moscow, Russia

    A. S. Shashkov & S. N. Senchenkova

  3. All-Russian Collection of Microorganisms, Russian Academy of Sciences, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, 142290, Pushchino, Moscow Region, Russia

    L. V. Dorofeeva & L. I. Evtushenko

Authors
  1. N. V. Potekhina
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  2. A. S. Shashkov
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  3. S. N. Senchenkova
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  4. L. V. Dorofeeva
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  5. L. I. Evtushenko
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Correspondence to N. V. Potekhina.

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Original Russian Text © N. V. Potekhina, A. S. Shashkov, S. N. Senchenkova, L. V. Dorofeeva, L. I. Evtushenko, 2012, published in Biokhimiya, 2012, Vol. 77, No. 11, pp. 1546–1555.

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Potekhina, N.V., Shashkov, A.S., Senchenkova, S.N. et al. Structure of hexasaccharide 1-phosphate polymer from Arthrobacter uratoxydans VKM Ac-1979T cell wall. Biochemistry Moscow 77, 1294–1302 (2012). https://doi.org/10.1134/S0006297912110089

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  • DOI: https://doi.org/10.1134/S0006297912110089

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