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Cell Wall Galactofuranan of the Paenarthrobacter Actinobacteria

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Abstract—

The composition and structure of the cell wall glycopolymers of Paenarthrobacter species (P. aurescens VKM Ac-1105T, P. histidinovorans VKM Ac-1978T, and P. nicotinovorans VKM Ac-1988T, previously assigned to the genus Arthrobacter) were studied. All strains under study were found to contain a neutral polysaccharide, (1 → 6)-linked β-D-galactofuranan with the 2,3-diacetamido-2,3-dideoxy-α-glucopyranose (α-GlcpNAc3NAc) residues that glycosylate most of the hydroxyl groups at C-2 of galactofuranose. Acid hydrolysates of the cell walls contained galactose, glucose, arabinose, glucosamine, and galactosamine. The polysaccharide structure was established by chemical and NMR spectroscopic methods. The galactofuranan structure has not been previously described for representatives of other Actinobacteria genera. The data obtained expand our understanding of the chemical structure of microbial cell walls and may be used in taxonomy of actinobacteria, especially to differentiate at the phenotype level and to justify the description of new genera in the family Micrococcaceae.

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REFERENCES

  1. Bock, K. and Pedersen, C., Carbon 13 nuclear magnetic resonance spectroscopy of monosaccharides, Adv. Carbohydr. Chem. Biochem., 1983, vol. 41, pp. 27–66.

    Article  CAS  Google Scholar 

  2. Busse, H.J., Family I. Corynebacteriaceae, in Bergey’s Manual of Systematic Bacteriology, 2nd ed., Whitman, W., Goodfellow, M., Kämpfer, P., Busse, H.-J., Trujillo, M., Ludwig, W., Suzuki, K.-I., and Parte, A., Eds., N.Y.: Springer, 2012, vol. 5, pp. 244–245.

  3. Busse, H.J., Review of the taxonomy of the genus Arthrobacter, emendation of the genus Arthrobacter sensu lato, proposal to reclassify selected species of the genus Arthrobacter in the novel genera Glutamicibacter gen. nov., Paeniglutamicibacter gen. nov., Pseudoglutamicibacter gen. nov., Paenarthrobacter gen. nov. and Pseudarthrobacter gen. nov., and emended description of Arthrobacter roseus, Int. J. Syst. Evol. Microbiol., 2016, vol. 66, pp. 9–37.

    Article  CAS  Google Scholar 

  4. Chun, J., Oren, A., Ventosa, A., Christensen, H., Arahal, D.R., da Costa, M.S., Rooney, A.P., Yi, H., Xu, X.W., De Meyer, S., and Trujillo, M.E., Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes, Int. J. Syst. Evol. Microbiol., 2018, vol. 68, pp. 461–466.

    Article  CAS  Google Scholar 

  5. Evtushenko, L.I. and Ariskina, E.V., Nocardioidaceae, in Bergey’s Manual of Systematics of Archaea and Bacteria, Whitman, W.B., Ed., 2015, pp. 1–18. https://doi.org/10.1002/9781118960608.fbm00042

    Book  Google Scholar 

  6. Goodfellow, M. and Jones, A.L., Corynebacteriales ord. nov., in Bergey’s Manual of Systematics of Archaea and Bacteria, Whitman, W.B., Ed., 2015, pp. 1–14. https://doi.org/10.1002/9781118960608.obm00009

    Book  Google Scholar 

  7. Goodfellow, M., Nocardiaceae, in Bergey’s Manual of Systematics of Archaea and Bacteria, Whitman, W.B., Ed., 2015, pp. 1–5. https://doi.org/10.1002/9781118960608.fbm00014

    Book  Google Scholar 

  8. Keddie, R.M. and Cure, G.L., Cell wall composition of coryneform bacteria, in Special Publications of the Society for General Microbiology I. Coryneform Bacteria, Bousfield, I.J., and Callely, A.G., Eds., London: Academic, 1978, pp. 47–83.

    Google Scholar 

  9. Kohler, T., Xia, G., Kulauzovic, E., and Peschel, A., Teichoic acids, lipoteichoic acids, and related cell wall glycopolymers of Gram-positive bacteria, in Microbial Glycobiology: Structures, Relevance and Applications, Moran, A., Holst, O., Brennan, P., von Itzstein, M., Eds., Amsterdam: Elsevier, 2009, pp. 75–91.

    Google Scholar 

  10. Lipkind, G.M., Shashkov, A.S., Mamyan, S.S., and Kochetkov, N.K., The nuclear Overhauser effect and structural factors determining the conformations of disaccharide glycosides, Carbohydr. Res., 1988, vol. 181, pp. 1–12.

    Article  CAS  Google Scholar 

  11. Magee, J.G. and Ward, A.C., Mycobacterium, in Bergey’s Manual of Systematics of Archaea and Bacteria, Whitman, W.B., Ed., 2015, pp. 1–84. https://doi.org/10.1002/9781118960608.gbm00029

    Book  Google Scholar 

  12. Naumova, I.B., The teichoic acids of actinomycetes, Microbiol. Sci., 1988, vol. 5, pp. 275–279.

    CAS  PubMed  Google Scholar 

  13. Nouioui, I., Carro, L., Garcia-Lopez, M., Meier-Kolthoff, J.P., Woyke, T., Kyrpides, N.C., Pukall, R., Klenk, H.P., Goodfellow, M., and Goker, M., Genome-based taxonomic classification of the phylum Actinobacteria, Front. Microbiol., 2018, vol. 9, art. 2007. https://doi.org/10.3389/fmicb.2018.02007

    Article  PubMed  PubMed Central  Google Scholar 

  14. Potekhina, N.V., Shashkov, A.S., Senchenkova, S.N., Dorofeeva, L.V., and Evtushenko, L.I., Structure of hexasaccharide 1-phosphate polymer from Arthrobacter uratoxydans VKM Ac-1979T cell wall, Biochemistry (Moscow), 2012, vol. 77, pp. 1294–1302.

    Article  CAS  Google Scholar 

  15. Potekhina, N.V., Streshinskaya, G.M., Tul’skaya, E.M., and Shashkov, A.S., Cell wall teichoic acids in the taxonomy and characterization of Gram-positive bacteria, in Taxonomy of Prokaryotes, Methods in Microbiology, Rainey, F.A. and Oren, A., Eds., London: Academic, 2011, vol. 38, ch. 6, pp. 132–164.

    Google Scholar 

  16. Ryzhmanova, Y., Oshurkova, V., Troshina, O., Abashina, T., Ariskina, E., Avtukh, A., and Shcherbako-va, V., Anoxynatronum buryatiense sp. nov., an anaerobic alkaliphilic bacterium from a low mineralization soda lake in Buryatia, Russia, Int. J. Syst. Evol. Microbiol., 2017, vol. 67, pp. 4704–4709.

    Article  CAS  Google Scholar 

  17. Schade, J. and Weidenmaier, C., Cell wall glycopolymers of firmicutes and their role as nonprotein adhesins, FEBS Lett., 2016, vol. 590, pp. 3758–3771.

    Article  CAS  Google Scholar 

  18. Schäffer, C. and Messner, P., The structure of secondary cell wall polymers: how Gram-positive bacteria stick their cell walls together, Microbiology (SGM), 2005, vol. 151, pp. 643–651.

    Article  Google Scholar 

  19. Schleifer, K.H. and Kandler, O., Peptidoglycan types of bacterial cell walls and their taxonomic implications, Bacteriol. Rev., 1972, vol. 36, pp. 407–477.

    Article  CAS  Google Scholar 

  20. Schumann, P., Kämpfer, P., Busse, H.J., and Evtushenko, L.I., Proposed minimal standards for describing new genera and species of the suborder Micrococcineae, Int. J. Syst. Evol. Microbiol., 2009, vol. 59, pp. 1823–1849.

    Article  CAS  Google Scholar 

  21. Schumann, P., Peptidoglycan Structure, in Taxonomy of Prokaryotes, Methods in Microbiology, Rainey, F.A. and Oren, A., Eds., London: Academic, 2011, vol. 38, ch. 6, pp. 101–129.

    Google Scholar 

  22. Shashkov, A.S., Potekhina, N.V., Kachala, V.V., Senchenkova, S.N., Dorofeeva, L.V., and Evtushenko, L.I., A novel galactofuranan from the cell wall of Arthrobacter sp. VKM Ac-2576, Carbohydr. Res., 2012, vol. 352, pp. 215–218.

    Article  CAS  Google Scholar 

  23. Shashkov, A.S., Tul’skaya, E.M., Dorofeeva, L.V., Evtushenko, L.I., and Potekhina N.V., Two glycosyl 1-phosphate polymers and teichulosonic acid from Glutamicibacter protophormiae VKM Ac-2104T cell wall, Biochemistry (Moscow), 2020b, vol. 85, pp. 629–635.

    CAS  PubMed  Google Scholar 

  24. Shashkov, A.S., Tul’skaya, E.M., Streshinskaya, G.M., Dmitrenok, A.S., Potekhina, N.V., Senchenkova, S.N., Piskunkova, N.F., Dorofeeva, L.V., and Evtushenko, L.I., Rhamnomannans and teichuronic acid from cell wall of Rathayibacter tritici VKM Ac-1603T, Biochemistry (Moscow), 2020a, vol. 85, pp. 369–377.

    CAS  PubMed  Google Scholar 

  25. Taókeuchi, M. and Yokota, A., Cell-wall polysaccharides in coryneform bacteria, J. Gen. Appl. Microbiol., 1989, vol. 35, pp. 233–252.

    Article  Google Scholar 

  26. Takeuchi, M., Yokota, A., and Misaki, A., Comparative structures of the cell-wall polysaccharides of four species of the genus Microbacterium, J. Gen. Appl. Microbiol., 1990, vol. 36, pp. 255–271.

    Article  CAS  Google Scholar 

  27. Tul’skaya, E.M., Shashkov, A.S., Streshinskaya, G.M., Senchenkova, S.N., Potekhina, N.V., Kozlova, Yu. I., and Evtushenko, L.I., Teichuronic and teichulosonic acids of Actinomycetes, Biochemistry (Moscow), 2011, vol. 76, pp. 736–744.

    PubMed  Google Scholar 

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Funding

The work was supported by the Ministry of Science and Higher Education of the Russian Federation.

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Authors and Affiliations

  1. Biological Faculty, Moscow State University, 119192, Moscow, Russia

    N. V. Potekhina & E. M. Tul’skaya

  2. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia

    A. S. Shashkov

  3. All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia

    E. V. Ariskina, L. V. Dorofeeva & L. I. Evtushenko

Authors
  1. N. V. Potekhina
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  2. A. S. Shashkov
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  3. E. M. Tul’skaya
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  4. E. V. Ariskina
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  5. L. V. Dorofeeva
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  6. L. I. Evtushenko
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Correspondence to N. V. Potekhina.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by E. Makeeva

Abbreviations: HSQC, proton-detected heteronuclear single quantum correlation; J (SSIC), spin-spin interaction constant; ROESY, rotating-frame nuclear Overhauser effect correlation spectroscopy; COSY, correlation spectroscopy; TOCSY, total correlation spectroscopy; HMBC, heteronuclear multiple bond correlation; TSP, 3-(trimethylsilyl)-2,2,3,3-tetraduteropropionic acid sodium salt; δС, δН, chemical shifts of the 13C and 1H atoms, respectively.

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Potekhina, N.V., Shashkov, A.S., Tul’skaya, E.M. et al. Cell Wall Galactofuranan of the Paenarthrobacter Actinobacteria. Microbiology 90, 106–111 (2021). https://doi.org/10.1134/S0026261720060156

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