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Identification of overwintering vegetative cells of the bivalve-killing dinoflagellate Heterocapsa circularisquama in Uranouchi Inlet, Kochi Prefecture, Japan

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Abstract

Red tides of Heterocapsa circularisquama have led to serious damage of bivalve aquacultures in western coastal areas of Japan. To understand the whole picture regarding the ecology of this species, it is essential to clarify its overwintering mechanisms. In this study, the population dynamics of H. circularisquama were investigated from February 2004 to November 2005, and overwintering cells were identified for the first time in water columns of Uranouchi Inlet, Kochi Prefecture, Japan. Heterocapsa circularisquama cells were detected by the indirect fluorescent antibody technique using monoclonal antibodies that specifically recognize and react to this species. Vegetative cells were almost always detected from the first observation in February 2004 to November 2005 with temperatures of 10.5–30.6°C. During the period from winter to spring, this species survived in areas with a temperature higher than 10°C. The overwintering cells of H. circularisquama were isolated in March 2004, and identification was made via observation of the morphology and body scales of the cultured cells. These overwintering cells were identified as H. circularisquama and reacted to the monoclonal antibody. These results indicate that H. circularisquama can overwinter and survive throughout the year in a vegetative cell state in Uranouchi Inlet.

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References

  1. Matsuyama Y, Usuki H, Uchida T, Kotani Y. Effects of harmful algae on the early planktonic larvae of the oyster, Crassostrea gigas. In: Hallegraeff GA, Blackburn SI, Bolch CJ, Lewis RJ (eds). Harmful Algal Blooms 2000. IOC-UNESCO, Paris. 2001; 411–414.

    Google Scholar 

  2. Imai I, Yamaguchi M, Hori Y. Eutrophication and occurrences of harmful algal blooms in the Seto Inland Sea, Japan. Plankton Benthos Res. 2006; 1: 71–84.

    Article  Google Scholar 

  3. Tamai K. Current status of outbreaks and fisheries damages due to Heterocapsa circularisquama. Bull. Plankton Soc. Jpn. 1999; 46: 153–154 (in Japanese).

    Google Scholar 

  4. Matsuyama Y, Uchida T, Honjo T, Shumway SE. Impacts of the harmful dinoflagellate, Heterocapsa circularisquama, on shellfish aquaculture in Japan. J. Shellfish Res. 2001; 20: 1269–1272.

    Google Scholar 

  5. Matsuyama Y. Physiological and ecological studies on harmful dinoflagellate Heterocapsa circularisquama — I Elucidation of environmental factors underlying the occurrence and development of H. circularisquama red tide. Bull. Fish. Res. Agency 2003; 7: 24–105.

    Google Scholar 

  6. Nakanishi K, Onaka S, Kobayashi T, Masuda K. Bloom dynamics of Heterocapsa circularisquama in Ago Bay, Japan. Bull. Plankton Soc. Jpn. 1999; 46: 161–164 (in Japanese).

    Google Scholar 

  7. Shiraishi T, Hiroishi S, Nagai K, Go J, Yamamoto T, Imai I. Seasonal distribution of the shellfish killing dinoflagellate Heterocapsa circularisquama in Ago Bay monitored by indirect fluorescent antibody technique using monoclonal antibodies. Plankton Benthos Res. 2007; 2: 49–62.

    Article  Google Scholar 

  8. Hiroishi S, Nakai R, Seto H, Yoshida T, Imai I. Identification of Heterocapsa circularisquama by means of antibody. Fish. Sci. 2002; 68 (Suppl. I): 627–628.

    Google Scholar 

  9. Yamaguchi M, Itakura S, Nagasaki K, Matsuyama Y, Uchida T, Imai I. Effects of temperature and salinity on the growth of the red tide flagellates Heterocapsa circularisquama (Dinophyceae) and Chattonella verruculosa (Raphidophyceae). J. Plankton Res. 1997; 19: 1167–1174.

    Article  Google Scholar 

  10. Yamatogi T, Sakaguchi M, Matsuda M, Iwanaga S, Iwataki M, Matsuoka K. Effect on bivalve molluscs of a harmful dinoflagellate Heterocapsa circularisquama isolated from Omura Bay, Japan and its growth characteristics. Nippon Suisan Gakkaishi 2005; 71: 746–754.

    Article  Google Scholar 

  11. Horiguchi T. Heterocapsa circularisquama sp. nov. (Peridiniales, Dinophyceae): a new marine dinoflagellate causing mass mortality of bivalves in Japan. Phycol. Res. 1995; 43: 129–136.

    Article  Google Scholar 

  12. Iwataki M, Hansen G, Sawaguchi T, Hiroishi S, Fukuyo Y. Investigations of body scales in twelve Heterocapsa species (Peridiniales, Dinophyceae), including a new species H. pseudotriquetra sp. nov. Phycologia 2004; 43: 394–403.

    Article  Google Scholar 

  13. Chen LCM, Edelstein T, McLachlan J. Bonnemaisonia hamifera Hariot in nature and in culture. J. Phycol. 1969; 5: 211–220.

    Article  Google Scholar 

  14. Imai I, Itakura S, Matsuyama Y, Yamaguchi M. Selenium requirement for growth of a novel red tide flagellate Chattonella verruculosa (Raphidophyceae) in culture. Fish. Sci. 1996; 62: 834–835.

    CAS  Google Scholar 

  15. Yoshida T, Sako Y, Uchida A. Geographic differences in paralytic shellfish poisoning toxin profiles among Japanese populations of Alexandrium tamarense and A. catenella (Dinophyceae). Phycol. Res. 2001; 49: 13–21.

    Article  CAS  Google Scholar 

  16. Yoshimatsu S. The cysts of Fibrocapsa japonica (Raphidophyceae) found in bottom sediment in Harima-Nada, eastern Inland Sea of Japan. Bull. Plankton Soc. Jpn. 1987; 34: 25–31.

    Google Scholar 

  17. Imai I, Itoh K. Annual life cycle of Chattonella spp., causative flagellates of noxious red tides in the Inland Sea of Japan. Mar. Biol. 1987; 94: 287–292.

    Article  Google Scholar 

  18. Imai I, Itoh K. Cysts of Chattonella antiqua and C. marina (Raphidophyceae) in sediments of the Inland Sea of Japan. Bull. Plankton Soc. Jpn. 1988; 35: 35–44.

    Google Scholar 

  19. Imai I. Cyst formation of the noxious red tide flagellate Chattonella marina (Raphidophyceae) in culture. Mar. Biol. 1989; 103: 235–239.

    Article  Google Scholar 

  20. Imai I, Itakura S, Itoh K. Cysts of the red tide flagellate Heterosigma akashiwo, Raphidophyceae, found in bottom sediments of northern Hiroshima Bay, Japan. Nippon Suisan Gakkaishi 1993; 59: 1169–1173.

    Google Scholar 

  21. Nakata K, Iizuka S. A note on overwintering of a red tide dinoflagellate Gymnodinium nagasakiense (Dinophyceae) in Omura Bay, Kyusyu. Bull. Plankton Soc. Jpn. 1987; 34: 199–201.

    Google Scholar 

  22. Terada K, Ikeuchi H, Takayama H. Winter distribution of Gymnodinium nagasakiense (Dinophyceae) in coastal waters of Suo-Nada, Inland Sea of Japan. Bull. Plankton Soc. Jpn. 1987; 34: 201–204.

    Google Scholar 

  23. Honjo T, Yamamoto S, Nakamura O, Yamaguchi M. Annual cycle of motile cells of Gymnodinium nagasakiense and ecological features during the period of red tide development. In: Granéli E, Sundström B, Elder L, Anderson DM (eds). Toxic Marine Phytoplankton. Elsevier, New York. 1990; 165–170.

    Google Scholar 

  24. Itakura S, Imai I, Itoh K. Seasonal occurrence of the noxious red tide dinoflagellate Gymnodinium nagasakiense in Hiroshima Bay, Seto Inland Sea. Bull. Nansei Natl Fish. Res. Inst. 1990; 23: 27–33.

    Google Scholar 

  25. Honjo T, Imada N, Ohshima Y, Maema Y, Nagai K, Matsuyama Y, Uchida T. Potential transfer of Heterocapsa circularisquama with pearl oyster consignments. In: Reguera B, Blanco J, Fernandez ML, Wyatt T (eds) Harmful Algae. Xunta de Galicia and IOC of UNESCO, Vigo. 1998; 224–226.

    Google Scholar 

  26. Honjo T, Imada N. Future attention—Spread of Heterocapsa circularisquama red tides and its preventive measure. Bull. Plankton Soc. Jpn. 1999; 46: 180–181 (in Japanese).

    Google Scholar 

  27. Imada N, Honjo T, Shibata H, Oshima Y, Nagai K, Matsuyama Y, Uchida T. The quantities of Heterocapsa circularisquama cells transferred with shellfish consignments and the possibility of its establishment in new areas. In: Hallegraeff GM, Blackburn SI, Bolch CJ, Lewis RJ (eds). Harmful Algal Blooms 2000. IOC-UNESCO, Paris. 2001; 474–476.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Marine Environmental Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Oiwakecho, Kitashirakawa, Sakyo-ku, 606-8502, Kyoto, Japan

    Tomotaka Shiraishi & Ichiro Imai

  2. Laboratory of Microbiology, Department of Marine Bioscience, Faculty of Biotechnology, Fukui Prefectural University, 1-1 Gakuencho, 017-0003, Obama, Fukui, Japan

    Shingo Hiroishi

  3. Kochi Prefectural Fisheries Experimental Station, 1153-23 Haigata, Uranouchi, 785-0167, Susaki, Kochi, Japan

    Seiya Taino, Tetsu Ishikawa & Yoshihiro Hayashi

  4. Red Tide Research Division, National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5 Maruishi, 739-0452, Hatsukaichi, Hiroshima, Japan

    Setsuko Sakamoto & Mineo Yamaguchi

Authors
  1. Tomotaka Shiraishi
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  2. Shingo Hiroishi
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  3. Seiya Taino
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  4. Tetsu Ishikawa
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  5. Yoshihiro Hayashi
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  6. Setsuko Sakamoto
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  7. Mineo Yamaguchi
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  8. Ichiro Imai
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Correspondence to Ichiro Imai.

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Shiraishi, T., Hiroishi, S., Taino, S. et al. Identification of overwintering vegetative cells of the bivalve-killing dinoflagellate Heterocapsa circularisquama in Uranouchi Inlet, Kochi Prefecture, Japan. Fish Sci 74, 128–136 (2008). https://doi.org/10.1111/j.1444-2906.2007.01502.x

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  • DOI: https://doi.org/10.1111/j.1444-2906.2007.01502.x

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