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In vitro propagation of endangered Lippia filifolia mart. and schauer ex schauer

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Summary

This work describes an efficient micropropagation protocol of Lippia filifolia. Nodal segments cultivation in MS medium supplemented with 6-benzylaminopurine (4.5 μM)/α-naphthaleneacetic acid (NAA; 54nM) induced multiple shoots (in average 27 shoots per explant). Elongated shoots were rooted with NAA (0.11 μM) and they maintained ploidy level of the in vitro produced explants. The basic chromosome number were 2n=2x=24. Regenerated rooted shoots were successfully acclimatized under shading house conditions. This is the first report involving the establishment of a protocol for shoot multiplication and rooting for endangered L. filifolia, contributing for germplasm preservation of this species.

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References

  • Abena, A. A.; Diatewa, M.; Gakosso, G.; Gbeassor, M.; Hondi-Assah, T.; Ouamba, J. M. Analgesic; antipyretic and anti-inflammatory effects of essential oil of Lippia multiflora. Fitoterapia 74:231–236; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Ahmed, F.; Selim, M. S.; Das, A. K.; Choudhuri, M. S. K. Antiinflammatory and antinociceptive activities of Lippia nodiflora. Linn. Pharmazie. 59:329–330; 2004.

    CAS  Google Scholar 

  • Carvalho, C. R.; Saraiva, L. S. A new heterochromatin banding pattern revealed by modified HKG banding technique in maize chromosomes. Heredity 70:515–519; 1993.

    Google Scholar 

  • Compadre, C. M.; Robbins, E. F.; Kinghorn, D. The intensely sweet herb, Lippia dulcis Trev.: historical uses, field inquires, and constituents. J. Ethnopharmacol. 15:89–106; 1986.

    Article  PubMed  CAS  Google Scholar 

  • De Klerk, G. J. Rooting of microcuttings: theory and practice. In Vitro Cell. Dev. Biol.-Plant 38:415–422; 2002.

    Article  CAS  Google Scholar 

  • Gupta, S. K.; Khanuja, S. P. S.; Kumar, S. In vitro micropropagation of Lippia alba. Curr. Sci. 81:206–210; 2001.

    CAS  Google Scholar 

  • Juliani, H. R. Jr.; Koroch, A. R.; Juliani, H. R.; Trippi, V. S. Micropropagation of Lippia junelliana (Mold.) Tronc. Plant Cell Tiss. Organ Cult. 59:175–179; 1999.

    Article  Google Scholar 

  • Ket, N. V.; Hahn, E. J.; Park, S. Y.; Chakrabarty, D.; Paek, K. Y. Micropropagation of an endangered orchid Anoectochilus formosanus. Biol. Plant 48:339–344; 2004.

    Article  Google Scholar 

  • Macambira, L. M. A.; Andrade, C. H. S.; Matos, F. J. A.; Craveiro, A. A. Naphthoquinoids from Lippia sidoides. J. Nat. Prod. 49:310–312; 1986.

    Article  CAS  Google Scholar 

  • Moldenke, H. N. Monograph of Lippia. Phytologia 12:157; 1965.

    Google Scholar 

  • Murashige, T.; Skoog, F. A revised media for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Pascual, M. E.; Slowing, K.; Carretero, E.; Sánchez, M. D.; Villar, A. Lippia: traditional uses, chemistry and pharmacology: a review. J. Ethnopharmacol. 76:201–214; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Pham Huu Chanh, K.; Pham Huu Chanh, A. Comparative hypotensive effects of compounds extracted from Lippia multiflora leaves. Planta Med. 54:294–296; 1988.

    Article  PubMed  CAS  Google Scholar 

  • Phan, C. T.; Hegedus, P. Possible metabolic basis for the developmental anomaly called ‘vitreous plants’. Plant Cell Tiss. Organ Cult. 6:83–94; 1986.

    Article  CAS  Google Scholar 

  • Salimena-Pires, F. R. Verbenaceae da serra do Cipó, Minas Gerais, 1991. Dissertação (Mestrado em Botânica)—Universidade de São Paulo; 1991:302 p.

  • Skoog, F.; Miller, C. O. Chemical regulation of growth and organ formation in plant tissues culture in vitro. Symp. Soc. Exp. Biol. 11:118–131; 1957.

    Google Scholar 

  • Tavares, E. S.; Lopes, D.; Bizzo, H. R.; Lage, C.; Leitão, S. G. Kinetin enhanced linalool production by in vitro plantlets of Lippia alba. J. Essent. Oil Res. 16:405–408; 2004.

    CAS  Google Scholar 

  • Thorpe, T. A.; Harry, I. S.; Kumar, P. P. Application of micropropagation to forestry. In: Debergh, P. C.; Zimmerman, R. H., eds. Micropropagation. Dordrecht: Kluwer Academic Publishers; 1991:311–316.

    Google Scholar 

  • Viccini, L. F.; Costa, D. C. S.; Machado, M. A.; Campos, A. L. Genetic diversity among nine species of Lippia (Verbenaceae) based on RAPD markers. Plant Syst. Evol. 246:1–8; 2004.

    Article  CAS  Google Scholar 

  • Viccini, L. F.; Pierre, P. M. O.; Praça, M. M.; Costa, D. C. S.; Sousa, S. M.; Peixoto, P. H. P.; Salimena, F. R. G. Chromosome numbers in the genus Lippia. Plant Syst. Evol. 256:171–178; 2006.

    Article  Google Scholar 

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Authors and Affiliations

  1. Departamento de Botânica, ICB, UFJF, MG 36.036-900, Juiz de Fora, Brazil

    Paulo Henrique Pereira Peixoto, Fátima Regina Gonçalves Salimena & Leonardo da Silva Garcia

  2. Departamento de Biologia, ICB, UFJF, MG 36.036-900, Juiz de Fora, Brazil

    Marcelo de Oliveira Santos, Patrícia Maria de Oliveira Pierre & Lyderson Facio Viccini

  3. Departamento de Biologia Vegetal, CCB, UFV, MG 36.570-000, Viçosa, Brazil

    Wagner Campos Otoni

Authors
  1. Paulo Henrique Pereira Peixoto
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  2. Fátima Regina Gonçalves Salimena
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  3. Marcelo de Oliveira Santos
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  4. Leonardo da Silva Garcia
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  5. Patrícia Maria de Oliveira Pierre
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  6. Lyderson Facio Viccini
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  7. Wagner Campos Otoni
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Correspondence to Paulo Henrique Pereira Peixoto.

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Peixoto, P.H.P., Salimena, F.R.G., Santos, M.d.O. et al. In vitro propagation of endangered Lippia filifolia mart. and schauer ex schauer. In Vitro Cell.Dev.Biol.-Plant 42, 558–561 (2006). https://doi.org/10.1079/IVP2006814

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  • DOI: https://doi.org/10.1079/IVP2006814

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