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Micropropagation of fig (Ficus carica L.) ‘Roxo de Valinhos’ plants

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Summary

An in vitro protocol for Ficus carica cv. ‘Roxo de Valinhos’ was optimized. Nodal explants containing two buds were excised from field-grown mature plants, and transferred to different proliferation media consisting of combinations of distinct concentrations of activated charcoal with benzyladenine (BA), kinetin with gibberellic acid (GA3), and WPM (woody plant medium) with kinetin. The regular strength of WPM in combination with 0.5 mgl−1 kinetin was the best condition for shoot proliferation of Ficus carica ‘Roxo de Valinhos’ plants. The addition of activated charcoal in the medium completely inhibited shoot proliferation. The inclusion of BA in the medium induced excessive callus formation as well as small and vitrified shoots, while GA3 induced excessive elongation associated with vitrification, chlorosis, and tip-burned shoots.

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Authors and Affiliations

  1. Departamento de Agricultura, Universidade Federal de Lavras-UFLA, Caixa Postal 37, CEP 37.200-000, Lavras-MG, Brazil

    Chrystiane Borges Fráguas, Moacir Pasqual & Leonardo Ferreira Dutra

  2. Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias-Unesp, Via de Acesso Prof. Paulo Donato Castellane, s/n, CEP 14.884-900, Jaboticabal, SP, Brazil

    Jairo Osvaldo Cazetta

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  1. Chrystiane Borges Fráguas
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  2. Moacir Pasqual
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  3. Leonardo Ferreira Dutra
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  4. Jairo Osvaldo Cazetta
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Correspondence to Moacir Pasqual.

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Fráguas, C.B., Pasqual, M., Dutra, L.F. et al. Micropropagation of fig (Ficus carica L.) ‘Roxo de Valinhos’ plants. In Vitro Cell.Dev.Biol.-Plant 40, 471–474 (2004). https://doi.org/10.1079/IVP2004562

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  • DOI: https://doi.org/10.1079/IVP2004562

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