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Animal Models

The multi-organ origin of interleukin-5 in the mouse

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Abstract

Murine Ba/F3 cells were transfected with cDNA for the α-chain of the murine interleukin-5 (IL-5) receptor and cloned lines of these cells were able to proliferate in response to as little as 2.5 pg/ml of IL-5. The bioassay was demonstrated to be specific for IL-5 and was able to measure IL-5 produced in culture by organs from adult C57BL/6 and BALB/c mice. The highest levels of IL-5 were produced by lung tissue but thymus and bladder consistently produced IL-5 and more variable production was observed by the heart, spleen, muscle, bone shaft, uterus and testes. Bone marrow cells produced no detectable IL-5. Observed levels of production of IL-5 were similar when using organs from mice lacking high-affinity receptors for IL-5 and from nu/nu, RAG-1−/− and NOD/SCID mice lacking T lymphocytes. In inflammatory peritoneal exudates induced by the injection of casein plus bacteria, levels of induced IL-5 were higher if the mice lacked high-affinity receptors for IL-5. The data indicate that T lymphocytes are not the dominant cellular source of IL-5 in organ-conditioned media and that local IL-5 production can occur with a wide range of normal murine organs.

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Acknowledgements

We are indebted to Dr J-G Zhang, Bronwyn Roberts and Wendy Carter for their assistance in producing purified recombinant murine IL-5. This work was supported by the Carden Fellowship Fund of the Anti-Cancer Council of Victoria, the National Health and Medical Research Council, Canberra and the National Institutes of Health, Bethesda, Grant No. CA-22556.

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  1. The Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Victoria, 3050, Australia

    PJ Ryan, T Willson, WS Alexander, L Di Rago, S Mifsud & D Metcalf

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  1. PJ Ryan
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  2. T Willson
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  3. WS Alexander
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  4. L Di Rago
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  5. S Mifsud
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  6. D Metcalf
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Ryan, P., Willson, T., Alexander, W. et al. The multi-organ origin of interleukin-5 in the mouse. Leukemia 15, 1248–1255 (2001). https://doi.org/10.1038/sj.leu.2402173

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