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IL-24 inhibits the growth of hepatoma cells in vivo

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Abstract

The interleukin (IL)-24/melanoma differentiation associated gene-7 (mda-7) is a member of the IL-10 cytokine family. Introduction of the IL-24 gene into a variety of cancer cells suppresses their growth. It has not been shown, however, whether IL-24 can suppress the growth of hepatoma cells. The purpose of this study was to determine whether the mouse (m)IL-24 gene would suppress hepatoma cells in vivo after being delivered via intramuscular electroporation. After mice were given a subcutaneous dorsal injection of ML-1 hepatoma cells, the mIL-24 gene was delivered and suppressed tumor growth. On day 140, 60% of the mIL-24-treated mice (n=10) and 0% (n=10) of the untreated control mice had survived. We also generated a mouse-hepatoma model by injecting ML-1 cells into the spleen, which resulted in tumor metastasis in the liver. Intramuscular electroporation of mIL-24 also inhibited hepatoma-cell growth in the liver. On day 50, 90% of the experimental mice (n=10) and 40% (n=10) of the control mice had survived. Liver tumors in surviving experimental mice were 50% smaller than those in control mice. IL-24 also inhibited tumor vascularization. These results suggest that IL-24 has potential therapeutic value for hepatoma.

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Authors and Affiliations

  1. Graduate Institute of Biochemistry and Molecular Biology, Medical College, National Cheng Kung University, Tainan, Taiwan

    Wei-Yu Chen, Y-T Cheng, C-W Wang & Ming-Shi Chang

  2. Graduate Institute of Microbiology and Immunology, Medical College, National Cheng Kung University, Tainan, Taiwan

    H-Y Lei & C-P Chang

  3. Chi-Mei Medical Center, Tainan, Taiwan

    Ming-Shi Chang

  4. Institute of Basic Medical Science, Medical College, National Cheng Kung University, Tainan, Taiwan

    Wei-Yu Chen, H-Y Lei, C-P Chang & Ming-Shi Chang

Authors
  1. Wei-Yu Chen
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  2. Y-T Cheng
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  3. H-Y Lei
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  4. C-P Chang
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  5. C-W Wang
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Correspondence to Ming-Shi Chang.

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This study was supported by a grant from Chi-Mei Medical Center.

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Chen, WY., Cheng, YT., Lei, HY. et al. IL-24 inhibits the growth of hepatoma cells in vivo. Genes Immun 6, 493–499 (2005). https://doi.org/10.1038/sj.gene.6364233

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  • DOI: https://doi.org/10.1038/sj.gene.6364233

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