Abstract
Glioblastoma (GBM) is the most prevalent primary malignant brain tumor and is associated with extensive tumor cell infiltration into the adjacent brain parenchyma. However, there are limited targeted therapies that address this disease hallmark. While the invasive capacity of self-renewing cancer stem cells (CSCs) and their non-CSC progeny has been investigated, the mode(s) of migration used by CSCs during invasion is currently unknown. Here we used time-lapse microscopy to evaluate the migratory behavior of CSCs, with a focus on identifying key regulators of migration. A head-to-head migration assay demonstrated that CSCs are more invasive than non-CSCs. Time-lapse live cell imaging further revealed that GBM patient-derived CSC models either migrate in a collective manner or in a single cell fashion. To uncover conserved molecular regulators responsible for collective cell invasion, we utilized the genetically tractable Drosophila border cell collective migration model. Candidates for functional studies were generated using results from a targeted Drosophila genetic screen followed by gene expression analysis of the human homologs in GBM tumors and associated GBM patient prognosis. This strategy identified the highly conserved small GTPase, Rap1a, as a potential regulator of cell invasion. Alteration of Rap1a activity impaired the forward progress of Drosophila border cells during development. Rap1a expression was elevated in GBM and associated with higher tumor grade. Functionally, the levels of activated Rap1a impacted CSC migration speed out of spheres onto extracellular matrix. The data presented here demonstrate that CSCs are more invasive than non-CSCs, are capable of both collective and single cell migration, and express conserved genes that are required for migration and invasion. Using this integrated approach, we identified a new role for Rap1a in the migration of GBM CSCs.
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Introduction
Glioblastoma (GBM), the most prevalent primary malignant brain tumor in adults, remains one of the most lethal cancers, with a median survival of less than 2 years1,2,3,4. Barriers to effective treatment include the extensive infiltration of tumor cells throughout the brain and the high degree of inter- and intra-tumoral heterogeneity. GBM also contains self-renewing cancer stem cell (CSC) populations that are resistant to conventional therapies5,6. While many studies have identified mechanisms through which CSCs expand and are resistant to radiation and Temozolomide, the standard of care chemotherapy, less is known about the mechanisms that drive invasion. Several studies have reported that CSCs display elevated invasive potential over non-CSCs7,8,9. However, the differences between CSC and non-CSC invasion are unclear, as previous assessments have been done by comparing cell populations in isolation. A number of pathways drive GBM cell invasion, such as the Wingless/Int1 (Wnt) and TGF-β pathways10. Since these mechanisms are also associated with self-renewal11, the extent to which they drive invasion over self-renewal has yet to be determined. Increased invasion has been observed with anti-angiogenic therapies12,13. Additionally, GBM cells visualized in vivo tend to migrate along the luminal surface of blood vessels, white matter tracts, and the subarachnoid space below the meningeal covering of the brain and the brain parenchyma14. Studies in the perivascular environment revealed that cells migrate faster and induce further microvascular development15,16. These observations suggest that invasion itself may contribute to therapeutic resistance in GBM.
The mode by which GBM cells, particularly CSCs, migrate and invade the brain is poorly understood. Studies using tumor histology, live ex vivo tumor explants, and in vivo models demonstrate that cancer cells have the capacity to invade as single cells or as collectives, in which cells coordinate their movement by maintaining cell–cell contacts amongst small to large groups of cells17,18. Both single cell and collective cell modes of invasion have been observed in a multiple human tumors, including breast, thyroid, and lung carcinomas19,20,21,22,23,24. Additionally, migrating cancer cells are highly dynamic and can invade as single cells, loosely associated multicellular streams, collective nests or strands, or through expansive growth, with some cells changing their migration mode during movement in response to the surrounding tissue microenvironment17,25. Recent work has demonstrated that GBM cells can migrate as both single cells and as multicellular collectives, which may influence their capacity to infiltrate the surrounding brain parenchyma26,27. However, whether GBM CSCs themselves migrate and invade as cell collectives, and whether this differs from non-CSCs, has yet to be determined and is the focus of our studies.
Although collective cell invasion contributes to cancer, much of our current mechanistic understanding of how cells migrate as collective groups have been obtained by studying cells that move during normal development. Collective cell migration is a frequent mode in the embryo, where it contributes to the shaping and forming of many organs. Key examples include gastrulation to form the three embryonic germ layers, neural crest migration to give rise to craniofacial structures and the peripheral nervous system, zebrafish lateral line organ formation, and branching morphogenesis to elaborate tubular structures within organs (e.g. the mammary gland and mammalian lung)28,29. One of the best-studied models of developmental collective cell migration is Drosophila border cells, which migrate as a cohesive group of six to ten cells in the egg chamber, the functional unit of the ovary29. The border cell cluster migrates during oogenesis in two phases, both of which respond to specific ligands secreted by the oocyte: in the posterior phase, border cells undergo a long-range movement from the anterior end of the egg chamber to the oocyte at the posterior; in the dorsal phase, the cells undergo short-range migration along the oocyte towards the dorsal-anterior side of the egg chamber29,30. The ability to genetically manipulate and observe border cell migration in its native tissue environment in real time makes it a powerful tool for identifying conserved regulators of collective invasion in development and in cancer29,31,32. Moreover, the use of the Drosophila system has also recently been leveraged for studies to identify conserved molecular mechanisms that drive GBM cell proliferation, survival, and self-renewal33,34,35.
Here, we observed that GBM CSC models that migrate as collectives, individual cells or mixtures of both modes. Further, we used results from a Drosophila border cell screen to identify conserved genes that control cell migration, which represent potential targetable regulators of GBM CSC invasion. This approach identified Rap1a as a putative regulator. We found that human Rap1a levels were elevated in GBM, and altered Rap1a activity impacted CSC migration. These data demonstrate the ability to identify molecular regulators of migration and invasion of GBM CSCs, including Rap1a, using a multi-system approach.
Results
CSCs are more invasive than non-CSCs
Previous studies suggest CSCs have increased migration and invasion capacity compared to non-CSCs. However, these analyses were done separately and not in a competition assay that would normalize for confounding factors (e.g. media conditions or paracrine/autocrine factors). Therefore, we compared differentially labeled CSCs and non-CSCs in a head-to-head co-culture ECM-based cell invasion assay (Fig. 1a). We used an approach previously shown to assess breast cancer co-culture invasion36. We labeled CSCs and non-CSCs, then seeded them and overlaid the cells with a 3D extracellular matrix. We then added a chemoattractant on top. Using this system, we compared patient-derived GBM CSC models (T387, T4121, and T3691), versus their corresponding non-CSC progeny, which were independently derived from patient-derived xenograft (PDX) models. After 24 h, we assessed the extent of invasion into the matrix along the chemokine gradient via confocal imaging. In all models, we observed significantly more invasion by CSCs compared to non-CSCs (Fig. 1b, c). CSCs exhibited 2- to 5-fold increase in migration versus non-CSCs. These results thus demonstrate that CSCs are more invasive than non-CSCs when compared in identical conditions.
Different CSC lines display different modes of migration
To assess how CSCs invade in terms of single cell and collective cell migration, we analyzed how CSCs exit a sphere culture. In this assay, eight separate CSC models were grown as spheres before being introduced to an extracellular matrix coated surface and then imaged using time-lapse microscopy. All CSCs were able to migrate away from the original sphere. Some models migrated individually (T4121), whereas others migrated as cell collectives, most often as cohesive “finger-like” projections or small nests (T387 and L1; Fig. 2, Supplemental Fig. 1). In the collectively migrating lines, the cells consistently stayed connected, with N-cadherin enrichment at contacts between cells (Fig. 2, Supplemental Fig. 2). The majority of CSC models, displayed a mixture of migration modes, with cells moving away from the tumor sphere both as individuals and as collectives, most often in small cell groups (T3691, T3832, GBM10, L0, and T1919; Fig. 2, Supplemental Fig. 1). These data demonstrate that CSCs employ different modes of cell invasion, highlighting another phenotype that displays inter-tumor and intra-tumor heterogeneity.
Identification of candidate CSC invasion genes via border cell migration
Given the observation that CSCs display different modes of cell invasion, we sought to determine the underlying mechanisms driving CSC migration patterns. Drosophila border cells represent a genetically tractable model of collective cell migration within an intact tissue. Many genes known to regulate border cell migration are highly conserved in humans and have been implicated in cancer31,37,38. During mid-oogenesis, six to ten epithelial follicle cells are recruited to form the cohesive border cell cluster, which migrates as a coordinated unit over the course of about four hours towards the oocyte located at the posterior of the egg chamber39.
Recently, we performed an RNA interference (RNAi) screen targeting PDZ domain-containing genes to identify regulators of border cell collective migration40, which provided a starting point to identify candidate GBM migration genes. Proteins that contain the PDZ protein-protein interaction domain facilitate the formation of multi-protein scaffolding complexes with conserved roles in signaling, cell polarization, and adhesion, making them excellent candidates to regulate the collective migration of normal and cancer cells. The majority of Drosophila PDZ- (PSD95/Dlg/ZO1-) domain genes were screened for the ability to promote border cell migration40 (Fig. 3a). This screen identified high-confidence PDZ-domain-containing genes (multiple transgenic RNAi lines targeting the gene were able to disrupt migration; Supplemental Table 1: Group 1 genes) and lower confidence genes (only one RNAi line per gene disrupted migration; Supplemental Table 1: Group 2 genes). We further included well-characterized genes known to promote border cell migration and/or interact with PDZ domain genes (Supplemental Table 1: Group 3 genes)30,37.
We identified human orthologs for these 40 Drosophila migration genes using FlyBase and the Drosophila RNAi Screening Center Integrative Ortholog Prediction Tool (DIOPT; http://www.flyrnai.org/diopt)41. Human orthologs were used to interrogate the TCGA-GBMLGG dataset in order to find overlapping genes that were relevant in both the migration model and glioma patients. Genes whose high expression correlated with decreased patient survival were compared to the list of genes that decreased border cell migration (Fig. 3b; Supplemental Table 1). Twenty-three genes fit these criteria and were candidates for further functional tests in border cells and GBM CSCs. Notably, genes encoding small GTPases, or targets of small GTPases, were well-represented (Fig. 3b). Small GTPases are known to promote both single cell and collective cell migration, and many interact with PDZ-domain proteins42,43,44. For example, the high-confidence hit LIMK1 is downstream of Rac (human Rac1 and Rac2), which promotes membrane protrusions in migrating cells45,46. Similarly, Cdc42 stimulates actin-rich protrusions and can polarize migrating cells through direct binding to Par-6 (human PARD6B/PARD6G), one of the strongest hits from the border cell screen47,48,49, and has a well-described role in GBM invasion50.
As a validation of candidate genes (Fig. 3a, b; Supplemental Table 1), we confirmed that the small GTPase Cdc42 was required for migration. In border cells, expression of a dominant-negative (DN) mutant form of Cdc42 (Cdc42N17; DN Cdc42) severely blocked migration, with most border cells stopping along the migration pathway and failing to reach the oocyte (Supplemental Fig. 3a–c). Live border cells expressing DN Cdc42 overall had trouble initiating migration compared to controls (Supplemental Fig. 3c). Further, Cdc42 controlled migratory protrusions (Supplemental Fig. 3d). We found that DN Cdc42 border cell clusters extended more protrusions, and these protrusions were more persistent, with a longer lifetime than those produced by control border cells. These data are consistent with a recent study that found that Cdc42 promotes cell–cell communication amongst border cells, resulting in only one border cell at the front of the cluster being able to extend a productive protrusion, which facilitates migration of the entire collective to the oocyte51. Increasing the levels of Cdc42 via overexpression of wild-type (WT) Cdc42 also modestly impaired movement to the oocyte, although to a lesser extent than DN Cdc42 (Supplemental Fig. 3a, c). These results suggest that optimal levels of the Cdc42 small GTPase are required for migration in vivo as previously reported51.
Similarly, in GBM, we reduced the activity of Cdc42 in T387 cells using a specific chemical inhibitor, ML 14152. Decreased Cdc42 reduced the cell migration out of a CSC sphere (Supplemental Fig. 4a), but not cell viability, survival or proliferation (Supplemental Fig. 4b–d). Our results are consistent with recent work that found that knockdown of Cdc42 by siRNA decreased GBM invasion50. Further, constitutively active Cdc42 reduced survival in a PDX model of glioma, while increasing the invasive capacity in sphere exit assays.
Rap1a levels are elevated in GBM patients and correlate with tumor grade
Having established that this integrated approach can identify genes important for GBM CSC migration, we next focused on a candidate gene, human Rap1a, which is less characterized in collective cell migration. We and others recently showed that inhibition of the Drosophila Rap1a homolog (Rap1) disrupted border cell migration to the oocyte due to defects in actin-rich protrusions and altered cell–cell adhesion53,54. Rap1a is also regulated by the screen multi-hit gene, PDZ-GEF40 (ortholog of human RapGEF2/PDZ-GEF1 and RapGEF6/PDZ-GEF2; Supplementary Table 1). Further, expression of constitutively active (CA) Rap1a impaired movement of the border cells to the oocyte (Fig. 3c)53,54. Thus, similar to Cdc42, having the proper levels of Rap1a is critical for collective migration of border cells. In GBM, Rap1a expression was increased compared to non-tumor tissue using the TCGA database (Fig. 3f). Increased Rap1a expression also correlated with GBM compared to other glioma tumors (Fig. 3g), as well as increasing tumor grade (Fig. 3h). Additionally, when compared across the pan-cancer dataset, GBM had elevated levels of Rap1a compared with other common cancers, although it was not ubiquitously increased across all cancers (Fig. 3e). Survival analysis in the GBMLGG dataset also revealed that Rap1a expression levels correlate with a poor prognosis (Fig. 3d).
Activated Rap1a alters migration of CSCs
Assessment of Rap1a in CSCs compared to their non-CSC progeny across multiple models revealed that Rap1a was elevated in models that migrate collectively or as a mix of collective and single cells (T387, T3691, T3832; Fig. 4a). However, T4121, a model that migrated predominantly as single cells has lower Rap1A in CSCs compared on non-CSCs (Fig. 4a). On the basis that Rap1a levels are generally elevated in GBM, and that Drosophila Rap1 is required for collective migration of border cells (Fig. 3)53, we determined how altering Rap1a levels and/or activity impacted CSC migration. As described above, T387 and T3691 CSCs migrate as collective fingers and small clusters, while T4121 CSCs migrate as single cells (Fig. 2). Both increasing the levels of wild-type Rap1a (WT) and expressing the constitutively activated Rap1a (63E) mutant caused reduced migration in T387 and T3691when compared to the untransfected control. In comparison with overexpression of wild-type Rap1a, constitutively active Rap1a did not alter the migration rate in T387 but reduced the migration rate in T3691 (Fig. 4b–e). However, dominant-negative (N17) Rap1a induced a slight increase in migration rate compared to other overexpression conditions and had a similar migration rate to untransfected control conditions in T387 (Fig. 4d). In T3691, dominant-negative Rap1a had increased migration compared to constitutively active conditions and similar levels to wild-type overexpression conditions (Fig. 4e). T4121, which displayed single cell migration, was not impacted by modulation of Rap1a levels (Fig. 4f). These data suggest that levels of active Rap1a are critical for CSC migration in models that rely on a collective or mixed mode of cell migration.
Discussion
Cancer cells migrate individually or as collectives18,19,20,29,55. The mode of migration may be critical for how well tumor cells, including those in GBM, invade the surrounding tissue and disseminate. Recent studies found that GBM cells can migrate collectively26,27, although whether GBM CSCs specifically used this type of migration was an open question. Our data indicate that a variety of patient-derived GBM CSC models can migrate individually and as mixtures of single cells and collectives. Interestingly, previous studies indicated that CSCs are likely more invasive than non-CSCs in GBM7,8,9. However, these analyses were done separately, so it was unknown whether CSCs could outcompete non-CSCs during invasion. Here, we directly compared the invasive capacity of CSCs to non-CSCs. Our results with this head-to-head assay allowed for greater control and comparison, and showed that CSCs are more invasive than non-CSCs. Currently it is unknown whether the mode of CSC invasion directly contributes to progression of GBM in patients. Recent work by the Friedl group demonstrated that human glioma cells can invade as multicellular groups, as well as individual cells, into 3D astrocyte scaffolds, mouse brain slice cultures, mouse brain xenografts, and in human tumor samples26,27. Specifically, glioma cells maintain cell–cell connections while moving along both blood vessels and the astrocyte-rich brain stroma26. Thus, it is likely that collective invasion of GBM CSCs helps disseminate tumors into the brain parenchyma and vasculature14, although this has yet to be directly tested.
As GBM invasion is a dynamic and multi-step process, the molecular mechanisms are still poorly understood. Our results highlight unique opportunities to apply knowledge about migration and invasion from model organisms to human diseases such as GBM. Border cell migration during Drosophila oogenesis has become a valuable tool for studying collective cell migration due to the wealth of genetic and cell biological methods that allow for wild-type and mutant cells to be imaged ex vivo while migrating within the native tissue56. We used an informed approach to identify genes required for border cell migration that may have a role in GBM. In particular, we focused on the highly conserved PDZ-domain encoding genes, many of which have known or suspected roles in human tumorigenesis and cancer cell invasion57,58,59. Of these PDZ genes, plus related genes such as small GTPases with validated functions in border cells30, we identified a number of conserved genes whose elevated expression correlated with decreased GBM patient survival. These results, as well as recent work by others, indicate that the small GTPases Cdc42 and Rap1A are needed for the migration of both border cells and GBM CSCs50,51,53,54. In addition, the differences in expression between CSCs and non-CSCs as well as functional manipulations suggest that Rap1A is operating more in collective migration as compared to single cell migration. Moreover, Rap1a expression is higher in the mesenchymal subtype (accessed via TCGA; data not shown), which has a high propensity for invasion60. Further work will be needed to determine the roles, if any, of the additional identified genes on GBM tumor growth, migration, and/or invasion. Given the high conservation of human disease genes in Drosophila, especially those implicated in tumor invasion and metastasis31, our results further suggest that border cell migration can be used to identify additional relevant regulators of GBM cancer cell migration and invasion.
Rap1a GTPase is known to regulate the migration of both normal cells and cancer cells61,62. Rap1 belongs to the Ras family of small GTPases, but is regulated by distinct guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs), and generally has Ras-independent functions62. We found that constitutively active Rap1a decreased the migration speed of CSCs compared to dominant-negative Rap1a. However, elevated Rap1a levels correlate with worse GBM patient outcome. Additionally, Rap1 increases U87MG glioma spheroid invasion on collagen in response to platelet-derived growth factor (PDGF) stimulation63. One way to reconcile these counterintuitive findings is to consider the possibility that Rap1a may have different roles in GBM CSCs versus non-CSCs, as well as unknown effects on tumor progression in vivo versus cell culture. Moreover, it is also possible that CSCs have an optimized level of Rap1a activity, and our overexpression conditions compromised this equilibrium. We hypothesize that speed and migration persistence could impact how tumors with elevated Rap1a expression invade the brain. Although we transiently increased Rap1a expression using a wild-type Rap1a construct and similarly observed slower migration, this may not reflect what happens in GBM tumors in the native environment. Chronic elevation of Rap1a expression in GBM tumors could be worse for disease progression than transient activation. Interestingly, Rap1a has been shown to be important for cellular proliferation in the context of in vivo GBM tumor growth64. It will be important to test whether tumors that overexpress Rap1a are more infiltrative in vivo, whether the main function of Rap1a in GBM is in cell proliferation64, or whether some combination of tumor growth, migration, and invasion is at play. The effect that Rap1a activity has on GBM patient survival is likely to be complex, and not simply one in which increased Rap1a expression increases tumor invasion and thus decreases patient survival.
Overall, our data provide a rationale for additional studies on the role of Rap1a in GBM migration and invasion. Studies based on reducing cell migration and invasion should be a high priority due to the limited availability of anti-migration treatments, which remains difficult to target and is a driver of recurrence. Finally, our approach highlights the ability to leverage model organisms to identify key processes that drive GBM invasion and highlight a paradigm that could be applied to many other key cancer processes.
Materials and methods
Isolation of CSCs
CSCs were isolated from surgically obtained adult GBM specimens and transiently amplified by implantation of unsorted tumor cells into immunocompromised mice as PDXs. All specimens were generated under approved Institutional Review Board protocols. For PDX dissociation, single cells were prepared from the bulk tumor by a Papain dissociation kit (Worthington Biochemical) as per the manufacturer’s protocol and cultured using previously reported culturing methods65,66, with CSCs being enriched via CD133 beads (Miltenyi Biotec) and non-CSCs being devoid of CD133 cell surface expression. Cells were maintained in serum-free growth media supplemented with EGF and bFGF (“neurobasal complete media”) as spheres or adherent on Geltrex solution (Thermo Fisher Scientific). The following PDX models, and their molecular subtype (if known), were used in this study: T387 (mesenchymal), T3832 (proneural), T3691 (proneural), T4121 (classical), T1919 (undetermined), L1 (undetermined), L0 (undetermined), and GBM10 (undetermined). All cell lines were authenticated by STR profiling and routinely tested for mycoplasma contamination.
Head-to-head invasion assay
The head-to-head invasion assay was adapted from Goswami et al.36 with minor modifications. First, cancer cells were stained with either Vybrant DiO or DiI cell labeling solutions (Thermo Fisher Scientific), according to manufacturer’s directions. 150,000 to 200,000 cells in neurobasal complete media, from two distinct cell types, were added at 1:1 dilution to 35 mm glass bottom dishes (MatTek Corporation) that were coated with a dilute layer of Geltrex matrix. After 24 h, media was removed and a thick, 80 µl layer of Geltrex matrix was layered over the adherent cells. Dishes were returned to the incubator for 30 min, in order for the matrix to solidify. Then, the dishes were flooded with 3 mL of neurobasal complete media. The following day, live cells were visualized using a PerkinElmer UltraView VOX Spinning Disk Confocal Imaging System on a Nikon DM1-6000 SD inverted microscope using a 10×/NA 0.7 air objective. A 100-µm z-stack image at 5-µm increments was obtained. An extended x–z projection was then obtained using the Volocity software (PerkinElmer) and invasion was quantified using ImageJ (NIH, Bethesda, MD, USA) as the percentage of Vybrant dye signal found above 20 µm from the bottom of the dish, compared with total fluorescence within the field.
Immunofluorescence
For immunofluorescence analysis of adherent cultures, cells were fixed with 4% paraformaldehyde (Millipore Sigma, Burlington, MA, USA) at room temperature for 15 min, washed three times with phosphate-buffered saline (PBS) and blocked with 5% normal goat serum (Millipore) in PBS plus 0.1% Triton X-100 for 1 h. Cells were incubated with an anti-N-cadherin (1:200; 13116S, Cell Signaling) antibody. Cells were washed three times with PBS plus 0.1% Triton X-100 (Millipore) and incubated with appropriate secondary antibody (1:100 anti-mouse IgG3 Alexa 488 (# A-21151) and 1:300 goat anti rabbit Alexa 568 (# A-11011); Thermo Fisher Scientific). Nuclei were counterstained with DAPI solution, a coverslip added, and mounted on slides using VECTASHIELD Hardset mounting media (Vector Labs). Fluorescence micrographs were acquired using a Leica SP5 confocal microscope, and images were processed in Adobe Photoshop CS6 (Adobe).
The Drosophila strain slbo-GAL4, UAS-mCD8:GFP was crossed to w1118 (control) or the following UAS-transgene flies to drive expression in border cells: UAS-Rap1V12 (constitutively active Rap1), UAS-Cdc42N17 (dominant-negative Cdc42), UAS-Cdc42 (wild-type Cdc42). Detailed information about Drosophila strains can be found in FlyBase (http://flybase.org/). Ovaries from the correct progeny of these crosses were dissected, fixed in 4% methanol-free formaldehyde (Polysciences) in phosphate buffer, and stained for anti-E-cadherin (DCAD2, Developmental Studies Hybridoma Bank (DSHB), University of Iowa) at 1:10 dilution using standard protocols67. Secondary antibodies used were anti-mouse (A-11004) or anti-rat Alexa 568 (A-11077) at 1:400 (Thermo Fisher Scientific). Nuclei were labeled using 0.05 μg/ml DAPI (Millipore). Ovaries were further dissected and mounted on slides in Aqua-PolyMount (Polysciences), followed by imaging on a Zeiss AxioImager Z1 microscope. Images were taken using a Zeiss Axiocam 503 camera, the Zeiss ApoTome2 module, and a 20 ×0.75 NA objective, using either Zeiss AxioVision 4.8 or Zen software.
Time-lapse microscopy of border cell migration in ex vivo cultured egg chambers
Live imaging was performed essentially as previously described68,69. Briefly, Drosophila egg chambers were dissected from whole ovaries and cultured in Schneider’s insect medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Millipore Sigma), 0.20 mg/mL insulin (Millipore), and penicillin/streptomycin (Thermo Fisher Scientific). Egg chambers were transferred onto a 50 mm Lumox® gas-permeable bottom dish (Sarstedt, Inc.), overlaid with a 22 ×22 mm coverslip and the edges sealed with halocarbon oil 27 (Millipore Sigma) to prevent evaporation.
Widefield fluorescence z-stack images (5 z-sections at 3μm apart) were captured every 2 min for the duration of border cell migration (about 3 to 4 h). The Zeiss Colibri light source (25% blue LED at 250 msec exposure, full frame ROI) was used to illuminate GFP, which was expressed in border cells using the GAL4-UAS system. Movies were created using the Zeiss Axiovision 4.8 “Inside 4D” software module or the ImageJ distribution Fiji (http://fiji.sc)70. For each time-lapse movie, concurrent z-sections in which the border cell cluster was in focus were merged. Image brightness and/or contrast were adjusted in Fiji.
Protrusions were defined manually, as in Sawant et al. 53. Essentially, the converted QuickTime movies were analyzed using Fiji. Cellular extensions that were longer than 10 pixels (at least about 4 µm) from the main cell body were considered to be “protrusions.” Only those protrusions that appeared within the first hour of each movie were tracked for analyses. Statistical tests were performed in GraphPad Prism 7.
Time-lapse microscopy of tumor spheres
Floating spheres were transferred into Geltrex-coated 6-well dishes. Live time-lapse movies of adherent CSC cells were acquired using a Leica DMI6000 inverted microscope and LAS X software v3.4.18368.2 (Leica Microsystems) equipped with a Hamamatsu ImageEM CCD camera and a Hamamatsu Orca Flash4 camera (Hamamatsu Photonics). Phase contrast images of multiple fields per well were collected every 15 min for 36 h using a 10×0.4 NA objective lens. Image processing and analysis was performed using the open source analysis program Fiji (64 bit, build 2014.11.25)70. Change in confluency of CSC cells over time was quantified for 3 different time points from each of the time-lapse movies as a measure of cell proliferation and migration. Selected phase contrast images were pre-processed by first converting them to 32 bit images and then using the Contrast Limited Adaptive Histogram Equalization (CLAHE) plugin to increase contrast and flatten image background. Image segmentation and quantification of confluency of the CLAHE processed phase contrast images was then completed using the PHANTAST plugin71.
Immunoblotting
Cell populations were lysed using NP-40 lysis buffer (containing PMSF, protease inhibitor cocktail, phosphatase inhibitor cocktail). Protein concentrations were calculated using a BCA protein assay (Pierce Biotechnology). After denaturation with Laemmli buffer (BioRad Laboratories), 20 mg of total protein were loaded on 10% polyacrylamide SDS-PAGE gels, transferred to polyvinyl difluoride membranes (Millipore) and probed using the following antibodies: SOX2 (Cell Signaling, 2748 S, 1:1000), Rap1a (Abcam, ab115776, 1:1000) and β-Actin (Santa Cruz Biotechnology, sc-47778, 1:5000) was used as a loading control. Species-specific horseradish peroxidase-conjugated secondary antibodies were used for detection (Invitrogen, 1:5000). Membranes were developed using ECL-2 reagent (Pierce Biotechnology).
Cell manipulations
CSCs were transiently transfected in 10 cm plates. Once cells achieved a confluency of about 75%, they were transfected with a preparation of OptiMEM, 14.37 µg of DNA, and FuGENE HD Transfection reagent (Promega). On day 2 after the transfection, cells were removed from their plates with Accutase (BioLegend) and counted. For Rap1a alterations, CSCs were transfected with RAP1 WT, RAP1 N17, RAP1 63E, and with an eGFP control. Cdc42 was inhibited in T387 cells using ML 141 (Sigma) at 200 μM. Rap1 WT and N17 constructs were obtained from previously published sources72. Rap1 63E was obtained from Addgene (Plasmid #32698) from previously published sources72,73. The eGFP plasmid was obtained from Lonza.
Cell viability assays
2000 cells were added to Geltrex-coated 96-well plates. Opaque clear bottom plates were used for both CellTiterGlo and CaspaseGlo (Promega) assays and regular cell culture plates were used for CyQUANT assay (Thermo Fisher Scientific). Cells were incubated at 37 °C and 5% CO2 for the duration of the experiment. Assays were completed according to manufacturer’s directions on 1- and 3-days post-plating or addition of the inhibitor. CaspaseGlo measurements were normalized to CellTiterGlo measurements.
Statistics
All statistical analyses were performed in GraphPad Prism 5.0 (GraphPad Software). Unless otherwise indicated, a student’s t test or one-way ANOVA was used and a p value of ≤0.05 was considered significant.
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Acknowledgements
This work used the Leica SP5 confocal/multi-photon microscope that was purchased with partial funding from National Institutes of Health SIG grant 1S10RR026820-01. This work was supported in part by a fellowship from the Kansas INBRE through the National Institutes of Health grant P20 GM103418 to Y.C., NIH R21 CA198254 to J.D.L. and J.A.M., and support from the Cleveland Clinic (including the Brain Tumor Research and Therapeutic Development Center of Excellence) and Case Comprehensive Cancer Center to J.D.L.
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Volovetz, J., Berezovsky, A.D., Alban, T. et al. Identifying conserved molecular targets required for cell migration of glioblastoma cancer stem cells. Cell Death Dis 11, 152 (2020). https://doi.org/10.1038/s41419-020-2342-2
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DOI: https://doi.org/10.1038/s41419-020-2342-2
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