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Runx3 expression in gastrointestinal tract epithelium: resolving the controversy

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Abstract

We reported earlier that RUNX3 is expressed in human and mouse gastrointestinal tract (GIT) epithelium and that it functions as a tumor suppressor in gastric and colorectal tissues. However, there have been conflicting reports describing the absence of Runx3 in GIT epithelial cells. A part of the controversy may be derived from the use of a specific antibody by other groups (referred to as G-poly). Here, we show further evidence to support our earlier observations and provide a possible explanation for this apparent controversy. We generated multiple anti-RUNX3 monoclonal antibodies and found that RUNX3 antibodies recognizing the RUNX3 N-terminal region (residues 1–234) react with RUNX3 in gastric epithelial cells, whereas those recognizing the C-terminal region (beyond residue 234) did not. G-poly primarily recognizes the region beyond 234 and hence, is unable to detect Runx3 in this tissue.

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  1. K Ito

    Present address: 4Current address: Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8588, Japan.,

Authors and Affiliations

  1. Institute of Molecular and Cell Biology, Proteos, Singapore

    K Ito, K-i Inoue & Y Ito

  2. Oncology Research Institute, National University of Singapore, Singapore

    K Ito & Y Ito

  3. Department of Biochemistry, School of Medicine, and Institute for Tumor Research, Chungbuk National University, Cheongju, Republic of Korea

    S-C Bae

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  1. K Ito
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  2. K-i Inoue
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  3. S-C Bae
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  4. Y Ito
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Correspondence to Y Ito.

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Ito, K., Inoue, Ki., Bae, SC. et al. Runx3 expression in gastrointestinal tract epithelium: resolving the controversy. Oncogene 28, 1379–1384 (2009). https://doi.org/10.1038/onc.2008.496

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  • DOI: https://doi.org/10.1038/onc.2008.496

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