Southern transfer and hybridization1 is used to study how genes are organized within genomes by mapping restriction sites in and around segments of genomic DNA. This protocol describes the first stages of Southern blotting: digestion of genomic DNA with restriction enzymes, separation of the resulting fragments by gel electrophoresis, and capillary transfer of the denatured fragments to a membrane2.
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References
Southern, E.M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517 (1975).
Reed, K.C. & Mann, D.A. Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res. 13, 7207–7221 (1985).
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Southern blotting: Capillary transfer of DNA to membranes. Nat Methods 1, 91–92 (2004). https://doi.org/10.1038/nmeth1004-91
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DOI: https://doi.org/10.1038/nmeth1004-91
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