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Exhaustive benchmarking of the yeast two-hybrid system

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Figure 1: Comparison of protein interaction methods used by Braun et al.1 and exhaustive Y2H testing.

References

  1. Braun, P. et al. Nat. Methods 6, 91–97 (2009).

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  2. Rajagopala, S.V., Hughes, K.T. & Uetz, P. Proteomics 9, 5296–5302 (2009).

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  3. Stellberger, T. et al. Proteome Sci. 8, 8 (2010).

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Acknowledgements

P. Braun (Dana-Farber Cancer Institute, Boston) kindly provided the gold-standard clones used in this project. S. Shtivelband helped in the initial phase of this project. S. Fields, T. Hazbun and M. Koegl made helpful comments on the manuscript. This project was supported by the Landesstiftung Baden-Württemberg (Germany), the US National Institutes of Health (RO1GM79710) and the European Union (HEALTH-F3-2009-223101).

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  1. Peter Uetz

    Present address: Present address: Proteros Biostructures, Martinsried, Germany.,

Authors and Affiliations

  1. J. Craig Venter Institute, Rockville, Maryland, USA

    Yu-Chi Chen, Seesandra Venkatappa Rajagopala & Peter Uetz

  2. Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany

    Thorsten Stellberger

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  1. Yu-Chi Chen
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  2. Seesandra Venkatappa Rajagopala
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  3. Thorsten Stellberger
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  4. Peter Uetz
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Correspondence to Peter Uetz.

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Supplementary Table 1 and Supplementary Data (PDF 8930 kb)

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Chen, YC., Rajagopala, S., Stellberger, T. et al. Exhaustive benchmarking of the yeast two-hybrid system. Nat Methods 7, 667–668 (2010). https://doi.org/10.1038/nmeth0910-667

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