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Hitting the target

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Thermal profiling in combination with quantitative proteomics makes it possible to identify cellular membrane protein targets of small molecules.

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Figure 1: Savitsky et al.8 reported that the thermal shift in the denaturation temperature of soluble proteins bound to small-molecule ligands can be detected with quantitative proteomics, providing a powerful method to identify targets of small molecules (method A).

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Authors and Affiliations

  1. Yves Leestemaker and Huib Ovaa are at the Netherlands Cancer Institute, Amsterdam, the Netherlands.,

    Yves Leestemaker & Huib Ovaa

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  1. Yves Leestemaker
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  2. Huib Ovaa
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Correspondence to Huib Ovaa.

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The authors declare no competing financial interests.

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Leestemaker, Y., Ovaa, H. Hitting the target. Nat Methods 12, 1127–1128 (2015). https://doi.org/10.1038/nmeth.3660

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