Abstract
We used a sensitive method based on tetramers of peptide and major histocompatibility complex II (pMHCII) to determine whether CD4+ memory T cells resemble the T helper type 1 (TH1) and interleukin 17 (IL-17)-producing T helper (TH17) subsets described in vitro. Intravenous or intranasal infection with Listeria monocytogenes induced pMHCII-specific CD4+ naive T cells to proliferate and produce effector cells, about 10% of which resembled TH1 or TH17 cells, respectively. TH1 cells were also present among the memory cells that survived 3 months after infection, whereas TH17 cells disappeared. The short lifespan of TH17 cells was associated with small amounts of the antiapoptotic protein Bcl-2, the IL-15 receptor and the receptor CD27, and little homeostatic proliferation. These results suggest that TH1 cells induced by intravenous infection are more efficient at entering the memory pool than are TH17 cells induced by intranasal infection.
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Acknowledgements
We thank S. Jameson, H. Chu and J. Taylor for reviewing the manuscript; J. Walter, J. McLachlan and A. Schmidt for technical assistance; and P. Champoux and N. Shah for flow cytometry sorting. Supported by the US National Institutes of Health (AI39614, AI66016 and AI27998 to M.K.J.; T32-AI07313 to A.J.P.; and T32-CA9138 to M.P.).
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M.P. designed the study, did experiments, analyzed data and wrote the manuscript; J.L.L., A.J.P., T.Z. and T.D. did experiments; P.P.C. designed experiments; and M.K.J. designed the study, analyzed data and wrote the manuscript.
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Pepper, M., Linehan, J., Pagán, A. et al. Different routes of bacterial infection induce long-lived TH1 memory cells and short-lived TH17 cells. Nat Immunol 11, 83–89 (2010). https://doi.org/10.1038/ni.1826
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DOI: https://doi.org/10.1038/ni.1826
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