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Recombinant Listeria monocytogenes expressing an immunodominant peptide fails to protect after intravaginal challenge with herpes simplex virus-2

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Abstract

Recombinant Listeria monocytogenes expressing a type-common herpes simplex virus (HSV) gB-peptide was shown previously to protect against footpad inoculation with HSV-1. We tested this construct for protection against vaginal challenge with HSV-2. Primed mice demonstrated strong recall responses, had modest reductions in HSV-2 DNA in vaginal mucosa, but were not protected from disease.

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Acknowledgments

This work was supported in part by the following grants to SSW: NIH-K08HD51584, a Wyeth Infectious Disease Society of America award, Puget Sound Partners for Global Health, and a March of Dimes Basil O’Conner starter research award. WJM was supported by NIH grant T32 AI007411 and by a Child Health Research Career Development Award through the Department of Pediatrics, Feinberg School of Medicine, and Children’s Memorial Research Center at Northwestern University. DMK is supported in part by NIH AI50132. We thank Dr. Gregg Milligan (University of Texas Medical Branch, Galveston, TX) for HSV-2 strain 186, which was grown and titered by Chris McClurkan.

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Authors and Affiliations

  1. Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA

    William J. Muller

  2. Department of Immunology, University of Washington School of Medicine, Seattle, WA, USA

    Nural N. Orgun

  3. Department of Medicine, University of Washington, Seattle, WA, USA

    Lichun Dong & David M. Koelle

  4. Department of Laboratory Medicine, University of Washington, Seattle, WA, USA

    David M. Koelle & Meei-Li Huang

  5. Program in Pathobiology, University of Washington, Seattle, WA, USA

    David M. Koelle

  6. Benaroya Research Institute, Seattle, WA, USA

    David M. Koelle

  7. Fred Hutchinson Cancer Research Center, Seattle, WA, USA

    David M. Koelle

  8. Departments of Pediatrics and Microbiology, University of Minnesota School of Medicine, Minneapolis, MN, USA

    Sing Sing Way

  9. Division of Infectious Diseases, Children’s Memorial Hospital, 2300 Children’s Plaza, Box 20, Chicago, IL, 60614, USA

    William J. Muller

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  1. William J. Muller
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  2. Nural N. Orgun
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Correspondence to William J. Muller.

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Muller, W.J., Orgun, N.N., Dong, L. et al. Recombinant Listeria monocytogenes expressing an immunodominant peptide fails to protect after intravaginal challenge with herpes simplex virus-2. Arch Virol 153, 1165–1169 (2008). https://doi.org/10.1007/s00705-008-0089-7

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  • DOI: https://doi.org/10.1007/s00705-008-0089-7

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