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Human mitochondrial transcription factor B1 methylates ribosomal RNA at a conserved stem-loop

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Abstract

Human mitochondrial transcription factor B1 (h-mtTFB1) has an unprecedented relationship to RNA methyltransferases. Here, we show that this protein methylates a conserved stem-loop in bacterial 16S rRNA and that the homologous sequence in the human mitochondrial 12S molecule is similarly modified. Thus, h-mtTFB1 appears to be dual-function protein, acting both as a transcription factor and an rRNA-modification enzyme.

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Figure 1: A ksgA− E. coli strain10 with or without the indicated h-mtTFB1 plasmids (full-length h-mtTFB1 in pBluescript KS; Stratagene) were tested for resistance to kasugamycin as described5.
Figure 2: h-mtTFB1 methylates bacterial rRNA at a conserved stem-loop that is similarly modified in human mitochondria.

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Acknowledgements

The authors thank A. Vila-Sanjurjo for supplying the ksgA− bacterial strain. This work was supported by an R01 grant awarded to G.S.S. and an National Research Service Award to V.M. from the US National Institutes of Health.

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Authors and Affiliations

  1. Department of Biochemistry, Rollins Research Center, Emory University School of Medicine, Atlanta, 30322-3050, Georgia, USA

    Bonnie L. Seidel-Rogol, Vicki McCulloch & Gerald S. Shadel

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  1. Bonnie L. Seidel-Rogol
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  2. Vicki McCulloch
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  3. Gerald S. Shadel
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Correspondence to Gerald S. Shadel.

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The authors declare no competing financial interests.

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Seidel-Rogol, B., McCulloch, V. & Shadel, G. Human mitochondrial transcription factor B1 methylates ribosomal RNA at a conserved stem-loop. Nat Genet 33, 23–24 (2003). https://doi.org/10.1038/ng1064

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