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Protein engineering

Making ubiquitin specific

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Conformational targeting enabled the creation of a ubiquitin variant that specifically inhibits the deubiquitinating enzyme ubiquitin-specific protease 7 (also known as HAUSP). Generation of such tools is essential to unravel the complexities of ubiquitin signaling, but how general is this approach?

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Figure 1: Generation of a ubiquitin variant with high affinity for USP7 in any state.

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References

  1. Komander, D. & Rape, M. Annu. Rev. Biochem. 81, 203–229 (2012).

    CAS  PubMed  Google Scholar 

  2. Zhang, Y. et al. Nat. Chem. Biol. 9, 10–11 (2013).

    Article  Google Scholar 

  3. Li, M. et al. Nature 416, 648–653 (2002).

    Article  CAS  PubMed  Google Scholar 

  4. Nicholson, B. & Suresh Kumar, K.G. Cell Biochem. Biophys. 60, 61–68 (2011).

    Article  CAS  PubMed  Google Scholar 

  5. Lange, O.F. et al. Science 320, 1471–1475 (2008).

    Article  CAS  PubMed  Google Scholar 

  6. Faesen, A.C. et al. Mol. Cell 44, 147–159 (2011).

    Article  CAS  PubMed  Google Scholar 

  7. Kaiser, S.E. et al. Nat. Methods 8, 691–696 (2011).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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  1. Titia K. Sixma is in the Division of Biochemistry, Netherlands Cancer Institute, Amsterdam, The Netherlands.,

    Titia K. Sixma

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  1. Titia K. Sixma
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Correspondence to Titia K. Sixma.

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Sixma, T. Making ubiquitin specific. Nat Chem Biol 9, 10–11 (2013). https://doi.org/10.1038/nchembio.1143

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