Skip to main content
SpringerLink
Log in

Regulation of cytokinesis by spindle-pole bodies

  • Brief Communication
  • Published:

From Nature Cell Biology

View current issue Submit your manuscript

Abstract

In the fission yeast Schizosaccharomyces pombe, cytokinesis is thought to be controlled by the daughter spindle-pole body (SPB) through a regulatory pathway named the septation initiation network (SIN). Here, we demonstrate that laser ablation of both, but not a single SPB, results in failure of cytokinesis. Ablation of only the daughter SPB often leads to activation of the SIN on the mother SPB and successful cytokinesis. Thus, either SPB can drive cytokinesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: Effects of SPB ablation on cytokinesis.

Similar content being viewed by others

References

  1. Krapp, A., Gulli, M. P. & Simanis, V. Curr. Biol. 14, R722–R730 (2004).

    Article  CAS  Google Scholar 

  2. Grallert, A., Krapp, A., Bagley, S., Simanis, V. & Hagan, I. M. Genes Dev. 18, 1007–1021 (2004).

    Article  CAS  Google Scholar 

  3. Morrell, J. C. et al. Curr. Biol. 14, 579–584 (2004).

    Article  CAS  Google Scholar 

  4. Sohrmann, M., Schmidt, S., Hagan, I. & Simanis, V. Genes Dev. 12, 84–94 (1998).

    Article  CAS  Google Scholar 

  5. Khodjakov, A., La Terra, S. & Chang, F. Curr. Biol. 14, 1330–1340 (2004).

    Article  CAS  Google Scholar 

  6. Khodjakov, A., Cole, R. W., Oakley, B. R. & Rieder, C. L. Curr. Biol. 10, 59–67 (2000).

    Article  CAS  Google Scholar 

  7. Sparks, C. A., Morphew, M. & McCollum, D. J. Cell Biol. 146, 777–790 (1999).

    Article  CAS  Google Scholar 

  8. Cerutti, L. & Simanis, V. J. Cell Sci. 112, 2313–2321 (1999).

    CAS  PubMed  Google Scholar 

  9. Le Goff, X., Motegi, F., Salimova, E., Mabuchi, I. & Simanis, V. J. Cell Sci. 113, 4157–4163 (2000).

    CAS  PubMed  Google Scholar 

  10. He, X., Patterson, T. E. & Sazer, S. Proc. Natl Acad. Sci. USA 94, 7965–7970 (1997).

    Article  CAS  Google Scholar 

  11. Wu, J. Q. & Pollard, T. D. Science 310, 310–314 (2005).

    Article  CAS  Google Scholar 

  12. Daga, R. R., Lahoz, A., Munoz, M. J., Moreno, S. & Jimenez, J. EMBO J. 24, 2436–2446 (2005).

    Article  CAS  Google Scholar 

  13. Mishra, M. et al. J. Cell Sci. 117, 3897–3910 (2004).

    Article  CAS  Google Scholar 

  14. Wu, J. Q., Kuhn, J. R., Kovar, D. R. & Pollard, T. D. Dev. Cell 5, 723–734 (2003).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by National Institutes of Health grants GMS 59363 (to A.K.), GMS 69670 (to F.C), and by the Human Frontiers Science Program grant RGP0064 (to A.K). We thank D. McCollum and V. Simanis for strains, and R. Daga for technical assistance.

Author information

Authors and Affiliations

  1. Wadsworth Center, Albany, 12201–0509, NY, USA

    Valentin Magidson & Alexey Khodjakov

  2. Microbiology Department, Columbia University College of Physicians and Surgeons, New York, 10032, NY, USA

    Fred Chang

  3. Marine Biological Laboratory, Woods Hole, 02543, MA, USA

    Fred Chang & Alexey Khodjakov

Authors
  1. Valentin Magidson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fred Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alexey Khodjakov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Fred Chang or Alexey Khodjakov.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Information

Supplemantary Figures S1, S2, S3, Supplementary Tables S1 and S2 (PDF 1491 kb)

Supplementary Information

Supplementary Movie 1 (MOV 556 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Magidson, V., Chang, F. & Khodjakov, A. Regulation of cytokinesis by spindle-pole bodies. Nat Cell Biol 8, 891–893 (2006). https://doi.org/10.1038/ncb1449

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/ncb1449

  • Springer Nature Limited

This article is cited by

Search

Navigation