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Rahman et al. reply

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Abstract

Replying to H. Vais, J. K. Foskett & D.-O. D. Mak Nature 478, doi:10.1038/nature10493 (2011)

We observe that under conditions designed to mimic those of an unstimulated cell, InsP3 causes InsP3R to assemble rapidly and reversibly into small clusters of about 4–5 channels, and that gating of lone and clustered InsP3R by InsP3 and Ca2+ is different. We speculate that dynamic assembly of InsP3R clusters by InsP3 may contribute to the genesis of elementary Ca2+ release events by both assembling clusters and re-tuning the behaviour of InsP3R within them1,2,3. Vais et al.4 report that they have been unable to replicate some of our findings.

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Figure 1: InsP 3 -evoked clustering of InsP 3 R alters their gating.

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References

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Authors and Affiliations

  1. Department of Pharmacology, Tennis Court Road, CB2 1PD, Cambridge, UK

    Taufiq Rahman & Colin W. Taylor

  2. Luxembourg Centre for Systems Biomedicine, 162a Avenue de la Faiencerie, University of Luxembourg, L-1511, Luxembourg

    Alexander Skupin

  3. Institute for Systems Biology, 401 Terry Avenue North, Seattle, 98103, Washington, USA

    Alexander Skupin

  4. § Mathematical Cell Physiology, Max Delbruck Center for Molecular Medicine, Robert-Rössle-Str, 10, 13092 Berlin, Germany ,

    Martin Falcke

Authors
  1. Taufiq Rahman
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  2. Alexander Skupin
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  3. Martin Falcke
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  4. Colin W. Taylor
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Contributions

T.R. performed the experiments and, with C.W.T., analysed the data. C.W.T. and T.R. wrote the paper with input from A.S. and M.F. The project was directed by C.W.T.

Corresponding author

Correspondence to Colin W. Taylor.

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Rahman, T., Skupin, A., Falcke, M. et al. Rahman et al. reply. Nature 478, E2–E3 (2011). https://doi.org/10.1038/nature10494

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