Skip to main content

Advertisement

SpringerLink
Log in

Harmonemia: a universal strategy for flow cytometry immunophenotyping—A European LeukemiaNet WP10 study

  • Letter to the Editor
  • Published:
Leukemia Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1

References

  1. Swerdlow SH, Campo E, Harris NL, Jaffe ES, Pileri SA, Stein H et al. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues, 4th edn. International Agency for Research on Cancer (IARC): Lyon, France, 2008.

    Google Scholar 

  2. Béné MC, Nebe T, Bettelheim P, Buldini B, Bumbea H, Kern W et al. Porwit immunophenotyping of acute leukemia and lymphoproliferative disorders: a consensus proposal of the European LeukemiaNet Work Package 10. Leukemia 2011; 25: 567–574.

    Article  Google Scholar 

  3. Craig FE, Foon KA . Flow cytometric immunophenotyping for hematologic neoplasms. Blood 2008; 111: 3941–3967.

    CAS  Google Scholar 

  4. Béné MC, Castoldi G, Knapp W, Ludwig WD, Matutes E, Orfao et al. Proposals for the immunological classification of acute leukemias. European Group for the Immunological Characterization of Leukemias (EGIL). Leukemia 1995; 9: 1783–1786.

    PubMed  Google Scholar 

  5. Kalina T, Flores-Montero J, van der Velden VH, Martin-Ayuso M, Böttcher S, Ritgen M et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia 2012; 26: 1986–2010.

    Article  CAS  Google Scholar 

  6. Solly F, Rigollet L, Baseggio L, Guy J, Borgeot J, Guérin E et al. Comparable flow cytometry data can be obtained with two types of instruments, Canto II, and Navios. A GEIL study. Cytometry A 2013; 83: 1066–1072.

    Article  Google Scholar 

  7. Preijers FW, Huys E, Favre C, Moshaver B . Establishment of harmonization in immunophenotyping: a comparative study of a standardized one-tube lymphocyte-screening panel. Cytometry B Clin Cytom 2014; 86: 418–425.

    Article  CAS  Google Scholar 

  8. Mathis S, Chapuis N, Borgeot J, Maynadié M, Fontenay M, Béné MC et al. Comparison of cross-platform flow cytometry minimal residual disease evaluation in multiple myeloma using a common antibody combination and analysis strategy. Cytometry B Clin Cytom 2015; 88: 101–109.

    Article  CAS  Google Scholar 

  9. GEIL National Meeting. Recommandations pour le process d’accréditation de la paillasse de cytométrie. Bordeaux, France, 2012.

Download references

Acknowledgements

This work was supported by Beckman Coulter, which provided all reagents. The authors are especially grateful to Antoine Pacheco, Sue Ann Molero, Jeannine Holden and Markus Kaymer for fruitful discussions.

Author information

Authors and Affiliations

  1. Hematology Laboratory, Bordeaux University Hospital, Bordeaux, France

    F Lacombe & K Allou

  2. Douglass Hanly Moir Pathology, Sonic Healthcare, Sydney, New South Wales, Australia

    E Bernal

  3. Department of Haematology, Addenbrookes Hospital, Cambridge, UK

    D Bloxham

  4. Immunophenotyping Laboratory, Cardiff University, Cardiff, UK

    S Couzens

  5. Department of Hematology Oncology, University of Pavia and IRCCS Policlinico San Matteo, Pavia, Italy

    M G D Porta

  6. Flow Cytometry Laboratory, Bristol Royal Infirmary, Bristol, UK

    U Johansson

  7. München Leukemia Labor, Munich, Germany

    W Kern

  8. St Bartholomew's and Royal London School of Medicine and Dentistry, London, UK

    M Macey

  9. Hematology Laboratory, Geneva University Hospital, Geneva, Switzerland

    T Matthes

  10. The Institute of Cancer Research, Royal Cancer Hospital, London, UK

    R Morilla

  11. Flow Cytometry Unit, Clinical Pathology Service, Coimbra University Hospital Centre, Coimbra, Portugal

    A Paiva

  12. Department of Hematology, Hospital Universitario Vall d’Hebron, Barcelona, Spain

    C Palacio

  13. Department of Laboratory Medicine-Laboratory for Hematology, Radboud University Medical Center, Nijmegen, The Netherlands

    F Preijers

  14. Department of Hematology and Oncology, Helios Klinikum Berlin Buch, Berlin, Germany

    R Ratei

  15. Laboratory Services (HUSLAB), University Central Hospital, University of Helsinki, Helsinki, Finland

    S Siitonen

  16. Department of Pathobiology and Laboratory Medicine, University of Toronto, University Health Network, Toronto General Hospital, Toronto, Ontario, Canada

    A Porwit

  17. Hematology Laboratory, Nantes University Hospital, Nantes, France

    M C Béné

Authors
  1. F Lacombe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E Bernal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D Bloxham
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S Couzens
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M G D Porta
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. U Johansson
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. W Kern
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. M Macey
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. T Matthes
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. R Morilla
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. A Paiva
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. C Palacio
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. F Preijers
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. R Ratei
    View author publications

    You can also search for this author in PubMed Google Scholar

  15. S Siitonen
    View author publications

    You can also search for this author in PubMed Google Scholar

  16. K Allou
    View author publications

    You can also search for this author in PubMed Google Scholar

  17. A Porwit
    View author publications

    You can also search for this author in PubMed Google Scholar

  18. M C Béné
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M C Béné.

Ethics declarations

Competing interests

The authors declare no conflict of interest.

Additional information

Supplementary Information accompanies this paper on the Leukemia website

Supplementary information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lacombe, F., Bernal, E., Bloxham, D. et al. Harmonemia: a universal strategy for flow cytometry immunophenotyping—A European LeukemiaNet WP10 study. Leukemia 30, 1769–1772 (2016). https://doi.org/10.1038/leu.2016.44

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/leu.2016.44

  • Springer Nature Limited

This article is cited by

Search

Navigation