Skip to main content

Advertisement

SpringerLink
Log in

A polymerase I palm in adenylyl cyclase?

  • Scientific Correspondence
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

Zhang et al.1 recently reported the long-awaited structure determination of one of the catalytic core domains of eukaryotic adenylyl cyclase, which promises a greater understanding of the regulatory mechanisms associated with the use of cyclic AMP as a second messenger. We have searched the Brookhaven Protein Data Bank2 using the program PROTEP3 and found that, far from having a completely novel fold, the fold of the domain bears an extraordinary resemblance to the ‘palm’ domains of the polymerase I family of prokaryotic DNA polymerases, including Escherichia coli DNA polymerase I4,5 and Thermus aquaticus ( Taq) polymerase6,7. The similarity has important implications for the function and evolution of eukaryotic adenylyl cyclases and related proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: Chain traces9,10 of a, the palm domain of Taq polymerase (ref. 6; PDB code, 1TAQ) and b, a monomer of ACYc (ref. 1; PDB code, 1AB8).

References

  1. Zhang, G., Liu, Y., Ruoho, A. E. & Hurley, J. H. Nature 386, 247–253 (1997).

    Article  ADS  CAS  Google Scholar 

  2. Bernstein, F. C.et al. J. Molec. Biol. 112, 535–542 (1977).

    Google Scholar 

  3. Grindley, H. M., Artymiuk, P. J., Rice, D. W. & Willett, P. J. Mol. Biol. 229, 707–721 (1993).

    Google Scholar 

  4. Ollis, D. L.et al. Nature 313, 762–766 (1985).

    Article  ADS  CAS  Google Scholar 

  5. Beese, L. S., Derbyshire, V. & Steitz, T. A. Science 260, 352–355 (1993).

    Google Scholar 

  6. Kim, Y.et al. Nature 376, 612–616 (1995).

    Article  ADS  CAS  Google Scholar 

  7. Eom, S. H., Wang, J. & Steitz, T. A. Nature 382, 278–281 (1996).

    Article  ADS  CAS  Google Scholar 

  8. Tang, W.-J., Stanzel, M. & Gilman, A. G. Biochemistry 34, 14563–14572 (1995).

    Google Scholar 

  9. Kraulis, P. J. J. Appl. Cryst. 24, 946–950 (1991).

    Google Scholar 

  10. Merritt, E. A. & Murphy, M. E. P. Acta Crystallogr. D 50, 869–873 (1994).

    Google Scholar 

  11. Orengo, C. A. & Thornton, J. M. Structure 1, 105–120 (1993).

    Google Scholar 

  12. Janin, J. Nature 365, 21 (1993).

    Article  ADS  CAS  Google Scholar 

  13. Murzin, A. G., Bressner, S. E., Hubbard, T. & Chothia, C. J. Mol. Biol. 247, 536–540 (1995).

    Google Scholar 

  14. Gibrat, J.-F., Madej, T. & Bryant, S. H. Curr. Opin. Struct. Biol. 6, 377–385 (1996).

    Google Scholar 

  15. Koch, K.-W., Eckstein, F. & Stryer, L. J. Biol. Chem. 265, 9659–9663 (1990).

    Google Scholar 

  16. Joyce, C. M. & Steitz, T. A. Annu. Rev. Biochem. 63, 777–822 (1994).

    Google Scholar 

  17. Yan, S.-Z., Huang, Z.-H., Shaw, R. S. & Tang, W.-J. J. Biol. Chem. 272, 12342–12349 (1997).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology and Biotechnology, and Department of Information Studies, Krebs Institute, University of Sheffield, Sheffield, S10 2TN, UK

    Peter J. Artymiuk, Andrew R. Poirrette, David W. Rice & Peter Willett

Authors
  1. Peter J. Artymiuk
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andrew R. Poirrette
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David W. Rice
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Peter Willett
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Artymiuk, P., Poirrette, A., Rice, D. et al. A polymerase I palm in adenylyl cyclase?. Nature 388, 33–34 (1997). https://doi.org/10.1038/40310

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/40310

  • Springer Nature Limited

This article is cited by

Search

Navigation