Abstract
INHIBITORY non-adrenergic non-cholinergic (NANC) nerves are thought to be important in the autonomie innervation of the gastrointestinal tract and other organ systems. The nature of their neurotransmitter is still debated. Speculation that nitric oxide (NO), formed from L-arginine in neuronal structures1 and other cells2, could act as a neurotransmitter, is not yet supported by demonstration of its release upon nerve stimulation. Using a superfusion bioassay, we report the release of a vasorelaxant factor upon stimulation of the NANC nerves3 in the canine ileocolonic junction. Several pieces of evidence, including the selectivity of the bioassay tissues, chemical instability, inactivation by super-oxide anion and haemoglobin, inhibition by NG-nitro-L-arginine (L-NNA)4 and potentiation by L-arginine all indicated that NO accounted for the biological activity of this transferable NANC factor.
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Bult, H., Boeckxstaens, G., Pelckmans, P. et al. Nitric oxide as an inhibitory non-adrenergic non-cholinergic neurotransmitter. Nature 345, 346–347 (1990). https://doi.org/10.1038/345346a0
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DOI: https://doi.org/10.1038/345346a0
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