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Blockage of αβ T-cell development by TCR γδ transgenes

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Abstract

T LYMPHOCYTES recognize antigens by means of T-cell receptors (TCR) composed of αβ (refs 1–3) or γδ heterodimers4–6. The mechanism governing the development of αβ- and γδ-bearing T cells from a common precursor T cell is so far unknown. It has been proposed that T-cell precursors rearrange their γ- and δ-chain genes first, and αβ T cells are generated only from those cells that fail to rearrange productively both γ- and δ-chain genes7,8. Our recent study on γδ-transgenic mice contradicted this hypothesis, however, and indicated that repression of γ-chain gene expression mediated by a transcriptional silencer element has a critical role in the generation of αβ T cells9. Here we report that the generation of a αβ T cells is severely blocked in transgenic mice carrying γ- and δ-chain transgenes without the associated silencer, thereby strengthening the validity of the silencer model of T-cell development.

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Author notes

  1. Motoya Katsuki: Department of DNA Biology, School of Medicine, Tokai University and Central Institute for Experimental Animals, 1430 Nogawa, Miyamae, Kanagawa 213, Japan

  2. Sjef Verbeek and Anton Berns: Division of Molecular Genetics of the Netherlands Cancer Institute and the Department of Biochemistry of the University of Amsterdam, Plesmanlaan 121, Amsterdam, The Netherlands

Authors and Affiliations

  1. Howard Hughes Medical Institute at Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachussets, 02139, USA

    Marc Bonneville, Isao Ishida, Peter Mombaerts, Motoya Katsuki, Sjef Verbeek, Anton Berns & Susumu Tonegawa

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  1. Marc Bonneville
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  2. Isao Ishida
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  3. Peter Mombaerts
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  4. Motoya Katsuki
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  5. Sjef Verbeek
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  6. Anton Berns
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  7. Susumu Tonegawa
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Bonneville, M., Ishida, I., Mombaerts, P. et al. Blockage of αβ T-cell development by TCR γδ transgenes. Nature 342, 931–934 (1989). https://doi.org/10.1038/342931a0

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