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Structure of the protein subunits in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3Å resolution

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Abstract

The molecular structure of the photosynthetic reaction centre from Rhodopseudomonas viridis has been elucidated using X-ray crystallographic analysis. The central part of the complex consists of two subunits, L and M, each of which forms five membrane-spanning helices. We present the first description of the high-resolution structure of an integral membrane protein.

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References

  1. Thornber, J. P., Cogdell, R. J., Seftor, R. E. B. & Webster, G. D. Biochim. biophys. Acta 593, 60–75 (1980).

    Article  CAS  Google Scholar 

  2. Prince, R. C., Leigh, J. S. & Dutton, L. Biochim. biophys. Acta 440, 622–636 (1976).

    Article  CAS  Google Scholar 

  3. Feher, G. & Okamura, M. Y. in The Photosynthetic Bacteria (eds Clayton, R. K. & Sistrom, W. R.) 349–386 (Plenum, New York, 1978).

    Google Scholar 

  4. Okamura, M. Y., Feher, G. & Nelson, N. in Photosynthesis: Energy Conversion by Plants and Bacteria (ed. Govindjee) 195–272 (Academic, New York, 1982).

    Book  Google Scholar 

  5. Parson, W. W. & Ke, B. in Photosynthesis: Energy Conversion by Plants and Bacteria (ed. Govindjee) 331–384 (Academic, New York, 1982).

    Book  Google Scholar 

  6. Hoff, A. J. in Light Reaction Path of Photosynthesis (ed. Fong, F. K.) 80–151, 322–326 (Springer, Berlin, 1982).

    Google Scholar 

  7. Parson, W. W. A. Rev. Biophys. Bioengng. 11, 57–80 (1982).

    Article  CAS  Google Scholar 

  8. Michel, H. J. molec. Biol. 158, 567–572 (1982).

    Article  CAS  Google Scholar 

  9. Zinth, W., Kaiser, W. & Michel, H. Biochim. biophys. Acta 723, 128–131 (1983).

    Article  CAS  Google Scholar 

  10. Deisenhofer, J., Epp, O., Miki, K., Huber, R. & Michel, H. J. molec. biol. 180, 385–398 (1984).

    Article  CAS  Google Scholar 

  11. Norris, J. R., Uphaus, R. A., Crespi, H. L. & Katz, J. J. Proc. natn. Acad. Sci. U.S.A. 68, 625–628 (1971).

    Article  ADS  CAS  Google Scholar 

  12. Holten, D., Windsor, M. W., Parson, W. W. & Thornber, J. P. Biochim. biophys. Acta 501, 112–126 (1978).

    Article  CAS  Google Scholar 

  13. Shuvalov, V. A. & Asadov, A. A. Biochim. biophys. Acta 545, 296–308 (1979).

    Article  CAS  Google Scholar 

  14. Shopes, R. J. & Wraight, C. A. Biochim. biophys. Acta 806, 348–356 (1985).

    Article  CAS  Google Scholar 

  15. Wang, B. C. Lecture Notes for School on Direct Methods and Macromolecular Crystallography7 (Medical Foundation of Buffalo, Buffalo, New York, 1983).

    Google Scholar 

  16. Peters, J., Welte, W. & Drews, G. FEBS Lett. 171, 267–270 (1984).

    Article  CAS  Google Scholar 

  17. Wiemken, V. & Bachofen, R. FEBS Lett. 166, 155–159 (1984).

    Article  CAS  Google Scholar 

  18. Michel, H., Deisenhofer, J., Miki, K., Weyer, K. & Lottspeich, F. in Structure and Function of Membrane Proteins (eds Quagliariello, E. & Palmieri, F.) 191–197 (Eisevier, Amsterdam, 1983).

    Google Scholar 

  19. Jones, T. A. J. appl. Crystallogr. 11, 268–272 (1978).

    Article  CAS  Google Scholar 

  20. Jones, T. A. & Liljas, L. Acta Crystallogr. A40, 50–57 (1984).

    Article  Google Scholar 

  21. Michel, H., Weyer, K. A., Gruenberg, H. & Lottspeich, F. EMBO J. 4, 1667–1672 (1985).

    Article  CAS  Google Scholar 

  22. Brunisholz, R. A., Wiemken, V., Suter, F. & Bachofen, R. Hoppe-Seyler's Z. Physiol. Chem. 365, 689–701 (1984).

    Article  CAS  Google Scholar 

  23. Rossmann, M. G. & Argos, P. J. molec. Biol. 105, 75–95 (1976).

    Article  CAS  Google Scholar 

  24. Paillotin, G., Vermeglio, A., Breton, J. Biochim. biophys. Acta 545, 249–264 (1979).

    Article  CAS  Google Scholar 

  25. Breton, J. in Proc. Feldafing Workshop 23–25 March 1985 (ed. Michel-Beyerle, M. E.) (Springer, Berlin, in the press).

    Google Scholar 

  26. Matthews, B. W., Ferma, R. E., Bolognesi, M. C. & Schmid, M. F. J. molec. Biol. 131, 259–285 (1979).

    Article  CAS  Google Scholar 

  27. Schmid, M. F., Tronrud, D. E. & Matthews, B. W. Chim. scripta 21, 67–69 (1983).

    CAS  Google Scholar 

  28. Bunker, G., Stern, E. A., Blankenship, R. E. & Parson, W. W. Biophys. J. 37, 539–551 (1982).

    Article  ADS  CAS  Google Scholar 

  29. Eisenberger, P., Okamura, M. Y. & Feher, G. Biophys. J. 37, 523–538 (1982).

    Article  ADS  CAS  Google Scholar 

  30. Gast, P., Michalski, T. J., Hunt, J. E., Norris, J. R. FEBS Lett. 179, 325–328 (1985).

    Article  CAS  Google Scholar 

  31. Pfister, K., Steinback, K. E., Gardner, G. & Arntzen, C. J. Proc. natn. Acad. Sci. U.S.A. 78, 981–985 (1981).

    Article  ADS  CAS  Google Scholar 

  32. Stein, R. R., Castellvi, A. L., Bogacz, J. P. & Wraight, C. A. J. cell. Biochem. 24, 243–259 (1984).

    Article  CAS  Google Scholar 

  33. Dismukes, G. C., Frank, H. A., Friesner, R. & Sauer, K. Biochim. biophys. Acta 764, 253–271 (1984).

    Article  CAS  Google Scholar 

  34. Debus, R. J., Okamura, M. Y. & Feher, G. Biophys. J. 47, 3a (1985).

    Google Scholar 

  35. Hol, W. G. J. Prog. Biophys. molec. Biol. 45, 149–195 (1985).

    Article  CAS  Google Scholar 

  36. Debus, R. J., Feher, G. & Okamura, M. Y. Biochemistry 24, 2488–2500 (1985).

    Article  CAS  Google Scholar 

  37. Ploegman, J. H., Drent, G., Kalk, K. H. & Hol, W. G. J. J. molec. Biol. 123, 439–457 (1978).

    Article  Google Scholar 

  38. Blundell, T. et al. Nature 289, 771–777 (1981).

    Article  ADS  CAS  Google Scholar 

  39. Mathews, F. S. Prog. Biophys. molec. Biol. 45, 1–56 (1985).

    Article  CAS  Google Scholar 

  40. Youvan, D. C., Bylina, E. J., Alberti, M., Begusch, H. & Hearst, J. E. Cell 37, 949–957 (1984).

    Article  CAS  Google Scholar 

  41. Williams, J. C., Steiner, L. A., Ogden, R. C., Simon, M. I. & Feher, G. Proc. natn.Acad. Sci. U.S.A. 80, 6505–6509 (1983).

    Article  ADS  CAS  Google Scholar 

  42. Williams, J. C., Steiner, L. A., Feher, G. & Simon, M. I. Proc. natn. Acad. Sci. U.S.A. 81, 7303–7307 (1984).

    Article  ADS  CAS  Google Scholar 

  43. Zurawski, G., Bohnert, H. J., Whitfield, P. R. & Bottomley, W. Proc. natn. Acad. Sci. U.S.A. 79, 7699–7703 (1982).

    Article  ADS  CAS  Google Scholar 

  44. Rasmussen, O. F., Bookjans, G., Stummann, B. M. & Henningsen, K. W. Pl. Molec. Biol. 3, 1984–1984 (1984).

    Article  Google Scholar 

  45. Rochaix, J.-D., Dron, M., Rahire, M., Malnoe, P. Pl. molec. Biol 3, 363–370 (1984).

    Article  CAS  Google Scholar 

  46. Alt, J., Morris, J., Westhoff, P. & Hermann, R. G. Curr. Genet. 8, 597–606 (1984).

    Article  CAS  Google Scholar 

  47. Holschuh, K., Bottomley, W. & Whitfield, P. R. Nucleic Acids Res. 12, 8819–8834 (1984).

    Article  CAS  Google Scholar 

  48. Michel, H., Deisenhofer, J. in Encyclopedia of Plant Physiology: Photosynthesis III Vol. 19 (eds Staehelin, A. C. & Arntzen, C. J.) (Springer, Berlin, in the press).

  49. Rao, J. K. M., Hargrave, P. A. & Argos, P. FEBS Lett. 156, 165–169 (1983).

    Article  CAS  Google Scholar 

  50. Lesk, A. M. & Hardman, K. D. Science 216, 539–540 (1982).

    Article  ADS  CAS  Google Scholar 

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Deisenhofer, J., Epp, O., Miki, K. et al. Structure of the protein subunits in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3Å resolution. Nature 318, 618–624 (1985). https://doi.org/10.1038/318618a0

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