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Glial cells express N-CAM/D2-CAM-like polypeptides in vitro

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Abstract

The joining together of neurites to form fascicles and the growth of axons along glial surfaces1,2 during early development suggest that neurone–neurone and neurone–glial adhesion interactions are of considerable importance for defining nerve tracts. In vitro studies have indicated that adhesion between neurones3–5 involves a glycoprotein that has been independently studied under the names of N-CAM (for neural cell adhesion molecule6–8), D2-CAM9 and BSP-2 (refs 10, 11). As N-CAM/D2-CAM appears to be a homophilic ligand12 that binds to N-CAM/D2-CAM polypeptide on adjacent cells, this glycoprotein is potentially important in adhesion interactions between any two N-CAM/D2-CAM-expressing cells. While it has been suggested that neurone–glial adhesion involves molecules other than N-CAM/D2-CAM13–15, it is known that N-CAM/D2-CAM antigenic determinants are expressed by glial cells in vivo16,17 and that injection of anti-N-CAM antibodies into the eye-cup of chick embryos disrupts normal patterns of neuritic apposition to glial endfeet in the developing optic stalk17. Do the molecules expressed by glia share restricted antigenic determinants, or binding domains, with N-CAM/D2-CAM, or are N-CAM/D2-CAM polypeptides expressed by glia? Here we present immunocytochemical evidence which suggests that all classes of macroglia express N-CAM/D2-CAM antigenic determinants on their surfaces and immunochemical analyses which indicate that the molecules expressed by purified astrocytes are closely similar, or identical, to at least some forms of N-CAM/D2-CAM obtained from whole brain or purified neurones. However, our results also suggest that different N-CAM/D2-CAM polypeptides may be separately expressed by neurones and astrocytes.

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Authors and Affiliations

  1. Department of Clinical Neurology, Institute of Neurology, Queen Square, London, WC1N 3BG, UK

    Mark Noble

  2. The Protein Laboratory, University of Copenhagen, 34 Sigurdsgade, DK-2200, Copenhagen N., Denmark

    Merete Albrechtsen, Claus Møllert, Joan Lyles & Elisabeth Bock

  3. Department of Treponematoses, Statens seruminstitut, DK-2300, Copenhagen S., Denmark

    Elisabeth Bock

  4. Centre d'lmmunologie, INSERM-CNRS de Marseille-Luminy, 13288, Marseille, Cedex 9, France

    Christo Goridis

  5. Department of Anatomy, Case Western Reserve University, School of Medicine, 2119 Abington Road, Cleveland, Ohio, 44106, USA

    Michiko Watanabe & Urs Rutishauser

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  1. Mark Noble
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  2. Merete Albrechtsen
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  3. Claus Møllert
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  4. Joan Lyles
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  5. Elisabeth Bock
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  6. Christo Goridis
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  7. Michiko Watanabe
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  8. Urs Rutishauser
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Noble, M., Albrechtsen, M., Møllert, C. et al. Glial cells express N-CAM/D2-CAM-like polypeptides in vitro. Nature 316, 725–728 (1985). https://doi.org/10.1038/316725a0

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