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Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex

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Abstract

The complete amino acid sequence of one of the polypeptide chains of the human T3/T-cell receptor complex, the T3 glycoprotein (T3-δ-chain) of relative molecular mass 20,000, was deduced from cDNA clones derived from HPB-ALL cells and a human T-cell clone. Inspection of the 171-amino acid sequence reveals a signal peptide, a 79-amino acid extracellular domain, one transmembrane region and a 44-amino acid intracellular domain which show no sequence homology with members of the T-cell receptor/immunoglobulin/MHC multi-gene family. The T3 δ-chain is coded for by a single-copy gene the expression of which is restricted to T lymphocytes in humans and mice.

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Author notes

  1. John E. Coligan: Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20205, USA

  2. Alexander F. Markham: ICI Pharmaceuticals Division, Mereside, Alderly Park, Macclesfield, Cheshire 5K10 4TY, UK

  3. Stuart Orkin: Department of Hematology, Children's Hospital Medical Center, Boston, Massachusetts 02115, USA

Authors and Affiliations

  1. Laboratory of Molecular Immunology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Peter van den Elsen, Beth-Ann Shepley, Jannie Borst, John E. Coligan, Alexander F. Markham, Stuart Orkin & Cox Terhorst

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  1. Peter van den Elsen
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  2. Beth-Ann Shepley
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  3. Jannie Borst
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  4. John E. Coligan
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  5. Alexander F. Markham
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  6. Stuart Orkin
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  7. Cox Terhorst
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van den Elsen, P., Shepley, BA., Borst, J. et al. Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex. Nature 312, 413–418 (1984). https://doi.org/10.1038/312413a0

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