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Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin

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Abstract

The nucleotide sequence of cloned cDNA for preproenkephalin from bovine adrenal medulla indicates that the precursor protein contains four copies of Met-enkephalin and one copy each of Leu-enkephalin, Met-enkephalin-Arg6 -Phe7 and Met-enkephalin-Arg6 -Gly7 -Leu8, a previously undetected opioid peptide. The enkephalin and extended enkephalin sequences are each bounded by paired basic amino acid residues. Preproenkephalin may represent a multi-hormone precursor, like the corticotropin-β-lipotropin precursor.

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Authors and Affiliations

  1. Department of Medical Chemistry, Kyoto University Faculty of Medicine, Kyoto, 606, Japan

    Masaharu Noda, Yasuji Furutani, Hideo Takahashi, Mitsuyoshi Toyosato & Shosaku Numa

  2. Institute for Immunology, Kyoto University Faculty of Medicine, Kyoto, 606, Japan

    Shigetada Nakanishi

  3. Pharmaceutical Institute, Keio University School of Medicine, Tokyo, 160, Japan

    Tadaaki Hirose & Seiichi Inayama

Authors
  1. Masaharu Noda
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  2. Yasuji Furutani
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  3. Hideo Takahashi
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  4. Mitsuyoshi Toyosato
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  5. Tadaaki Hirose
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  6. Seiichi Inayama
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  7. Shigetada Nakanishi
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  8. Shosaku Numa
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Noda, M., Furutani, Y., Takahashi, H. et al. Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin. Nature 295, 202–206 (1982). https://doi.org/10.1038/295202a0

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  • DOI: https://doi.org/10.1038/295202a0

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