Abstract
The P3J-HR-1 (HR-1) line contains unique Epstein–Barr viruses (EBVs) which fail to immortalize lymphocytes or to induce lymphomas1–4. Virus from Jijoye, the parent of the HR-1 clone, retains lymphocyte-immortalizing ability5,6. Because the HR-1 virus population is a mixture, it has not been possible to identify conclusively the genomic alterations responsible for the nontransforming phenotype7. Submolar fragments produced by restriction enzyme digestion and the variety of patterns found after partial denaturation are evidence for heterogeneity in HR-1 virion DNA8,9. To determine whether every cell carried this virus mixture, or whether the line consisted of cells carrying different variants, we separated the mixture by cell subcloning into new variants, which are found to be distinct from parental HR-1 virus both biologically and in genome structure.
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Heston, L., Rabson, M., Brown, N. et al. New Epstein–Barr virus variants from cellular subclones of P3J-HR-1 Burkitt lymphoma. Nature 295, 160–163 (1982). https://doi.org/10.1038/295160a0
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DOI: https://doi.org/10.1038/295160a0
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