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Phosphotyrosine-containing proteins isolated by affinity chromatography with antibodies to a synthetic hapten

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Abstract

Tyrosine-specific protein kinases seem to be involved critically in cellular transformation by tumour viruses1–4, and may also be involved in the cellular response to epidermal growth factor5. To facilitate the identification and isolation of phosphotyrosine-containing proteins (PT-proteins) we have sought to develop as a general reagent anti-O-phosphotyrosine antibodies. We show here that affinity chromatography with these antibodies is effective for the small-scale isolation of some PT-proteins, including p120, the transforming protein of Abelson murine leukaemia virus, and several unidentified proteins from Rous sarcoma virus (RSV)-transformed mouse fibroblasts.

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Authors and Affiliations

  1. Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    Alonzo H. Ross, David Baltimore & Herman N. Eisen

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  1. Alonzo H. Ross
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  2. David Baltimore
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  3. Herman N. Eisen
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Ross, A., Baltimore, D. & Eisen, H. Phosphotyrosine-containing proteins isolated by affinity chromatography with antibodies to a synthetic hapten. Nature 294, 654–656 (1981). https://doi.org/10.1038/294654a0

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  • DOI: https://doi.org/10.1038/294654a0

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