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Nucleotide sequence of the yeast plasmid

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Abstract

The nucleotide sequence of the yeast DNA plasmid (2µ circle) from Saccharomyces cerevisiae strain A364A D5 has been determined. The plasmid contains 6,318 base pairs, including two identical inverted repeats of 599 base pairs. Possible functions are suggested, and attributes of an improved vector for cloning foreign DNAs in yeast are discussed.

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References

  1. Clark-Walker, G. D. & Miklos, G. L. G. Eur. J. Biochem. 41, 359–365 (1974).

    Article  CAS  Google Scholar 

  2. Bak, A., Christiansen, C. & Christiansen, G. Biochim. biophys. Acta 269, 527–530 (1972).

    Article  CAS  Google Scholar 

  3. Gubbins, E. J., Newlon, C. S., Kann, M. D. & Donelson, J. E. Gene 1, 185–207 (1977).

    Article  CAS  Google Scholar 

  4. Livingston, D. M. & Klein, H. L. J. Bact. 129, 472–481 (1977).

    CAS  Google Scholar 

  5. Cameron, J. R., Phillipsen, P. & Davis, R. W. Nucleic Acids Res. 4, 1429–1448 (1977).

    Article  CAS  Google Scholar 

  6. Guerineau, M., Grandchamp, C. & Slonimski, P. P. Proc. natn. Acad. Sci. U.S.A. 73, 3030–3034 (1976).

    Article  ADS  CAS  Google Scholar 

  7. Hollenberg, C. P., Degelmann, A., Kustermann–Kuhn, B. & Royer, H. D. Proc. natn. Acad. Sci. U.S.A. 73, 2072–2076 (1976).

    Article  ADS  CAS  Google Scholar 

  8. Beggs, J. D., Guerineau, M. & Atkins, J. F. Molec. gen. Genet. 148, 287–294 (1976).

    Article  CAS  Google Scholar 

  9. Bell, L. & Byers, B. Proc. natn. Acad. Sci. U.S.A. 76, 3445–3449 (1979).

    Article  ADS  CAS  Google Scholar 

  10. Nelson, R. G. & Fangman, W. L. Proc. natn. Acad. Sci. U.S.A. 76, 6515–6519 (1979).

    Article  ADS  CAS  Google Scholar 

  11. Livingston, D. M. & Hahne, S. Proc. natn. Acad. Sci. U.S.A. 76, 3727–3731 (1979).

    Article  ADS  CAS  Google Scholar 

  12. Zakian, V. A., Brewer, B. J. & Fangman, W. L. Cell 17, 923–934 (1979).

    Article  CAS  Google Scholar 

  13. Livingston, D. M. & Kupfer, D. M. J. molec. Biol. 116, 249–260 (1977).

    Article  CAS  Google Scholar 

  14. Broach, J. R., Atkins, J. F., McGill, C. & Chow, L. Cell 16, 827–839 (1979).

    Article  CAS  Google Scholar 

  15. Hollenberg, C. P., Kustermann-Kuhn, B. & Royer, H. D. Gene 1, 33–47 (1976).

    Article  CAS  Google Scholar 

  16. Hollenberg, C. P. Molec. gen. Genet. 162, 23–34 (1978).

    Article  CAS  Google Scholar 

  17. Livingston, D. M. Genetics 86, 73–84 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Tabak, H. F. FEBS Lett. 84, 67–70 (1977).

    Article  ADS  CAS  Google Scholar 

  19. Beggs, J. D. Nature 275, 104–109 (1978).

    Article  ADS  CAS  Google Scholar 

  20. Hicks, J. B., Hinnen, A. H. & Fink, G. R. Cold Spring Harb. Symp. quant. Biol. 43, 1305–1314 (1978).

    Article  Google Scholar 

  21. Struhl, K., Stinchcomb, D. T., Scherer, S. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 76, 1035–1039 (1979).

    Article  ADS  CAS  Google Scholar 

  22. Gerbaud, C. et al. Gene 5, 233–253 (1979).

    Article  CAS  Google Scholar 

  23. Blanc, H., Gerbaud, C., Slonimski, P. P. & Guerineau, M. Molec. gen. Genet. 176, 335–342 (1979).

    Article  CAS  Google Scholar 

  24. Beggs, J. D., Van den Berg, J., Van Ooyen, A. & Weissmann, C. Nature 283, 835–840 (1980).

    Article  ADS  CAS  Google Scholar 

  25. Toh-e, A., Guerry-Kopecko, P. & Wickner, R. B. J. Bact. 141, 413–416 (1980).

    CAS  PubMed  Google Scholar 

  26. Bolivar, F., Rodriguez, R. L., Betlach, M. C. & Boyer, H. W. Gene 2, 75–93 (1977).

    Article  CAS  Google Scholar 

  27. Roychoudhury, R., Jay, E. & Wu, R. Nucleic Acids Res. 3, 863–877 (1976).

    Article  CAS  Google Scholar 

  28. Grunstein, M. & Hogness, D. S. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).

    Article  ADS  CAS  Google Scholar 

  29. Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  30. Korn, L. J., Queen, C. L. & Wegman, M. N. Proc. natn. Acad. Sci. U.S.A. 74, 4401–4405 (1977).

    Article  ADS  CAS  Google Scholar 

  31. Mahler, H. R. & Cordes, E. H. Biological Chemistry 2nd edn (Harper and Row, New York, 1971).

    Google Scholar 

  32. Hindley, J. & Phear, G. A. Nucleic Acids Res. 7, 361–375 (1979).

    Article  CAS  Google Scholar 

  33. Tinoco, I. et al. Nature new Biol. 246, 40–41 (1973).

    Article  CAS  Google Scholar 

  34. Arnott, S., Chandrasekaran, R., Birdsall, D. L., Leslie, A. G. W. & Ratliff, R. L. Nature 283, 743–745 (1980).

    Article  ADS  CAS  Google Scholar 

  35. Soeda, E., Arrand, J. R., Smolar, N., Walsh, J. E. & Griffin, B. E. Nature 283, 445–453 (1980).

    Article  ADS  CAS  Google Scholar 

  36. Sanger, F. et al. Nature 265, 687–695 (1977).

    Article  ADS  CAS  Google Scholar 

  37. Fiers, W. et al. Nature 273, 113–119 (1978).

    Article  ADS  CAS  Google Scholar 

  38. Strathern, J. N., Newlon, C. S., Herskowitz, I. & Hicks, J. B. Cell 18, 309–319 (1979).

    Article  CAS  Google Scholar 

  39. Crews, S., Ojala, D., Posakony, J., Nishiguchi, J. & Attardi, G. Nature 277, 192–198 (1979).

    ADS  CAS  Google Scholar 

  40. Benz, E. W., Reinberg, D., Vicuna, R. & Hurwitz, J. J. biol. Chem. 255, 1096–1106 (1980).

    CAS  PubMed  Google Scholar 

  41. Shortle, D. & Nathans, D. J. molec. Biol. 131, 801–817 (1979).

    Article  CAS  Google Scholar 

  42. Sanger, F. & Coulsen, A. R. FEBS Lett. 87, 107–110 (1978).

    Article  CAS  Google Scholar 

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Hartley, J., Donelson, J. Nucleotide sequence of the yeast plasmid. Nature 286, 860–864 (1980). https://doi.org/10.1038/286860a0

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  • DOI: https://doi.org/10.1038/286860a0

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