Abstract
The nucleotide sequence of the yeast DNA plasmid (2µ circle) from Saccharomyces cerevisiae strain A364A D5 has been determined. The plasmid contains 6,318 base pairs, including two identical inverted repeats of 599 base pairs. Possible functions are suggested, and attributes of an improved vector for cloning foreign DNAs in yeast are discussed.
Similar content being viewed by others
References
Clark-Walker, G. D. & Miklos, G. L. G. Eur. J. Biochem. 41, 359–365 (1974).
Bak, A., Christiansen, C. & Christiansen, G. Biochim. biophys. Acta 269, 527–530 (1972).
Gubbins, E. J., Newlon, C. S., Kann, M. D. & Donelson, J. E. Gene 1, 185–207 (1977).
Livingston, D. M. & Klein, H. L. J. Bact. 129, 472–481 (1977).
Cameron, J. R., Phillipsen, P. & Davis, R. W. Nucleic Acids Res. 4, 1429–1448 (1977).
Guerineau, M., Grandchamp, C. & Slonimski, P. P. Proc. natn. Acad. Sci. U.S.A. 73, 3030–3034 (1976).
Hollenberg, C. P., Degelmann, A., Kustermann–Kuhn, B. & Royer, H. D. Proc. natn. Acad. Sci. U.S.A. 73, 2072–2076 (1976).
Beggs, J. D., Guerineau, M. & Atkins, J. F. Molec. gen. Genet. 148, 287–294 (1976).
Bell, L. & Byers, B. Proc. natn. Acad. Sci. U.S.A. 76, 3445–3449 (1979).
Nelson, R. G. & Fangman, W. L. Proc. natn. Acad. Sci. U.S.A. 76, 6515–6519 (1979).
Livingston, D. M. & Hahne, S. Proc. natn. Acad. Sci. U.S.A. 76, 3727–3731 (1979).
Zakian, V. A., Brewer, B. J. & Fangman, W. L. Cell 17, 923–934 (1979).
Livingston, D. M. & Kupfer, D. M. J. molec. Biol. 116, 249–260 (1977).
Broach, J. R., Atkins, J. F., McGill, C. & Chow, L. Cell 16, 827–839 (1979).
Hollenberg, C. P., Kustermann-Kuhn, B. & Royer, H. D. Gene 1, 33–47 (1976).
Hollenberg, C. P. Molec. gen. Genet. 162, 23–34 (1978).
Livingston, D. M. Genetics 86, 73–84 (1977).
Tabak, H. F. FEBS Lett. 84, 67–70 (1977).
Beggs, J. D. Nature 275, 104–109 (1978).
Hicks, J. B., Hinnen, A. H. & Fink, G. R. Cold Spring Harb. Symp. quant. Biol. 43, 1305–1314 (1978).
Struhl, K., Stinchcomb, D. T., Scherer, S. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 76, 1035–1039 (1979).
Gerbaud, C. et al. Gene 5, 233–253 (1979).
Blanc, H., Gerbaud, C., Slonimski, P. P. & Guerineau, M. Molec. gen. Genet. 176, 335–342 (1979).
Beggs, J. D., Van den Berg, J., Van Ooyen, A. & Weissmann, C. Nature 283, 835–840 (1980).
Toh-e, A., Guerry-Kopecko, P. & Wickner, R. B. J. Bact. 141, 413–416 (1980).
Bolivar, F., Rodriguez, R. L., Betlach, M. C. & Boyer, H. W. Gene 2, 75–93 (1977).
Roychoudhury, R., Jay, E. & Wu, R. Nucleic Acids Res. 3, 863–877 (1976).
Grunstein, M. & Hogness, D. S. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).
Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Korn, L. J., Queen, C. L. & Wegman, M. N. Proc. natn. Acad. Sci. U.S.A. 74, 4401–4405 (1977).
Mahler, H. R. & Cordes, E. H. Biological Chemistry 2nd edn (Harper and Row, New York, 1971).
Hindley, J. & Phear, G. A. Nucleic Acids Res. 7, 361–375 (1979).
Tinoco, I. et al. Nature new Biol. 246, 40–41 (1973).
Arnott, S., Chandrasekaran, R., Birdsall, D. L., Leslie, A. G. W. & Ratliff, R. L. Nature 283, 743–745 (1980).
Soeda, E., Arrand, J. R., Smolar, N., Walsh, J. E. & Griffin, B. E. Nature 283, 445–453 (1980).
Sanger, F. et al. Nature 265, 687–695 (1977).
Fiers, W. et al. Nature 273, 113–119 (1978).
Strathern, J. N., Newlon, C. S., Herskowitz, I. & Hicks, J. B. Cell 18, 309–319 (1979).
Crews, S., Ojala, D., Posakony, J., Nishiguchi, J. & Attardi, G. Nature 277, 192–198 (1979).
Benz, E. W., Reinberg, D., Vicuna, R. & Hurwitz, J. J. biol. Chem. 255, 1096–1106 (1980).
Shortle, D. & Nathans, D. J. molec. Biol. 131, 801–817 (1979).
Sanger, F. & Coulsen, A. R. FEBS Lett. 87, 107–110 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hartley, J., Donelson, J. Nucleotide sequence of the yeast plasmid. Nature 286, 860–864 (1980). https://doi.org/10.1038/286860a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/286860a0
- Springer Nature Limited
This article is cited by
-
The 2 micron plasmid: a selfish genetic element with an optimized survival strategy within Saccharomyces cerevisiae
Current Genetics (2018)
-
Multilevel Control of Organelle DNA Sequence Length in Plants
Journal of Molecular Evolution (2008)
-
Utility of the FLP-FRT recombination system for genetic manipulation of rice
Plant Cell Reports (2005)
-
High-level expression of a sweet protein, monellin, in the food yeast Candida utilis
Nature Biotechnology (1997)
-
Chimeric evolution of the 2-?m genome in Saccharomyces cerevisiae
Journal of Molecular Evolution (1994)