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Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

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Abstract

The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the anti-metabolic drug trimethoprim.

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Authors and Affiliations

  1. Department of Genetics, Stanford University, Stanford, California, 94305

    Annie C. Y. Chang & Stanley N. Cohen

  2. Department of Medicine, Stanford University, Stanford, California, 94305

    Annie C. Y. Chang, Henry A. Erlich & Stanley N. Cohen

  3. Department of Biological Sciences, Stanford University, Stanford, California, 94305

    Jack H. Nunberg & Robert T. Schimke

  4. Department of Medical Microbiology, Stanford University, Stanford, California, 94305

    Annie C. Y. Chang

  5. Department of Pharmacology, Stanford University, Stanford, California, 94305

    Randal J. Kaufman

Authors
  1. Annie C. Y. Chang
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  2. Jack H. Nunberg
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  3. Randal J. Kaufman
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  4. Henry A. Erlich
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  5. Robert T. Schimke
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  6. Stanley N. Cohen
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Chang, A., Nunberg, J., Kaufman, R. et al. Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase. Nature 275, 617–624 (1978). https://doi.org/10.1038/275617a0

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  • DOI: https://doi.org/10.1038/275617a0

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